We narrowed to 1,409 results for: 4-Oct
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Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W
Plasmid#67986PurposeCas9 activity reporter with mCherry and BFPDepositorInsertsU6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEP4 E02S EM2K
Plasmid#20923Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4DepositorArticleUseEpisomal/ebnaTagsExpressionMutationPromoterAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W
Plasmid#67985PurposeCas9 activity reporter (control) with mCherry and BFPDepositorInsertU6gRNA cassette, PGKmCherryABFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W
Plasmid#67975PurposeCRISPR gRNA expression vector with an improved scaffold and puro/ZsG markersDepositorInsertU6gRNA cassette, PGKpuro2AZsG cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pInducer10b-HA-KRAS WT CO SR
Plasmid#164926PurposeExpresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequenceDepositorInsertKRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
UseLentiviralTagsHA tagExpressionMutation9 Valine codons are optimized and siRNA targetin…PromoterUbc promoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP
Plasmid#67990PurposeCRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPREDepositorInsertU6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W
Plasmid#67984PurposeCas9 activity reporter with GFP and BFPDepositorInsertsU6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMSOX17FNV
Plasmid#206382PurposeExpresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W
Plasmid#67983PurposeCas9 activity reporter (control) with GFP and BFPDepositorInsertU6gRNA cassette, PGKGFP2ABFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACTIN-Cxcl5-PGK-Cre
Plasmid#110278PurposeBicistronic lentivirus expressing Cxcl5 and CreDepositorInsertsUseCre/Lox and LentiviralTagsNo fusion genesExpressionMammalianMutationWild typePromoterB-actin and PgkAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA4
Plasmid#113133PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro GFP-REX1[degron]-IRES-mCherry
Plasmid#232240PurposeMammalian expression of REX1[degron]-GFP-IRES-mCherryDepositorInsertREX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationREX1 (aa 70-85)PromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rat SERCA2a (pMT2)
Plasmid#75183PurposeMammalian expression of ATP2A2DepositorInsertATP2A2
UseTagsExpressionMammalianMutationPromoteradenovirus major late promoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eYFP-FKBP-MAD1-AA
Plasmid#114038PurposeChemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)DepositorInsertHuman MAD1 (MAD1L1 Human)
UseTagseYFP-FKBP12ExpressionMammalianMutationMAD2 binding deficient MAD1 K541A/L543APromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SOCS6-WT
Plasmid#74496PurposeOverexpression of SOCS6 in mammalian cellsDepositorInsertSOCS6 (Socs6 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterunknownAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATF4 17: 5’ATF4.uORF1stopm1&2.GFP
Plasmid#21863DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStop codons between the colinear uORF1 and uORF2 …PromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
Rat SERCA2b (pMT2)
Plasmid#75184PurposeMammalian expression of ATP2A2DepositorInsertATP2A2
UseTagsExpressionMammalianMutationPromoteradenovirus major late promoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry
Plasmid#232246PurposeMammalian expression of delta73-82 REX1-GFP-IRES-mCherryDepositorInsertdelta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationdelta73-82PromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry
Plasmid#232245PurposeMammalian expression of I77P I78P REX1-GFP-IRES-mCherryDepositorInsertI77P I78P REX1-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationI77P I78PPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 4xUTR
Plasmid#40759PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert4 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iM
Plasmid#58972PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoter and IRESAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-FNIP1
Plasmid#72292PurposeoverexpressionDepositorInsertFNIP1 (FNIP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-FNIP1
Plasmid#72293PurposeoverexpressionDepositorInsertFNIP1 (FNIP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
CANE-LV envelope
Plasmid#86666PurposepCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirusDepositorInsertCANE-LV envelope
UseLentiviral, Mouse Targeting, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
HsCLYBL(D320A)-FLAG
Plasmid#111291Purposemammalian expressionDepositorInserthuman CLYBL D320A (CLYBL Human)
UseTagslinker and FLAG tag: TGGGSGGSGGSMDYKDDDDKExpressionMammalianMutationD320APromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pUC57mini ZM249M P90A _env eGFP
Plasmid#132939PurposepUC57mini ZM249M _env eGFP containing P90A mutant capsid sequenceDepositorInsertHIV-1ZM249M provirus (P90A) with env deletion, GFP
UseLentiviralTagsExpressionMutationP90APromoterAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMA7
Plasmid#79967PurposeTM-MAGE strainDepositorInsertsLambda Red recombinase beta subunit
DNA adenine methylase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPU-Empty-miR30-TRIM5
Plasmid#132936PurposeAll-in-one control rescue-TRIM5 shRNA knockdown vectorDepositorInsertcontrol shRNA
UseLentiviral and RNAiTagsHAExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK226
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HsCLYBL(aa23-340)-His
Plasmid#111288Purposebacterial expressionDepositorInserthuman CLYBL ( aa23-340) codon optimized for bacterial expression (CLYBL Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pYS2
Plasmid#117490PurposeGeneration of unmarked gene deletions in Mycobacterium smegmatisDepositorInsertgfp, hyg
UseDeletion generationTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDY1173_ADAR1p150
Plasmid#193192PurposeExpress full length ADAR1p150DepositorInsertADAR1p150 (ADAR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti_DCLK1
Plasmid#163625PurposeExpresses DCLK1DepositorInsertDCLK1 (DCLK1 Human)
UseLentiviralTagsV5ExpressionMutationPromotersv40Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only