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pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

Plasmid

#115795

Purpose

Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrIIA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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NbV5-Rluc8

Plasmid

#201480

Purpose

BRET detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

Rluc8-NbV5

Tags

Rluc8

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET19b-rDAD2 cPGFP(G164)

Plasmid

#165994

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rDAD2 cPGFP(G164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA3-TSC1

Plasmid

#19911

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Tsc1 (Tuberous sclerosis 1) (TSC1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pOTIII.EFGP.IGR3.6

Plasmid

#40866

Depositor

Harold Gainer

Article

Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

Insert

MOUSE OXYTOCIN GENE (Oxt Mouse)

Expression

Bacterial

Promoter

mouse oxytocin promoter

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pT3TS-miniTurboID-EGFP

Plasmid

#163847

Purpose

In vitro transcription of miniTurboID-EGFP. Parton lab clone JRT

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

In vitro transcription of rna

Tags

EGFP

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-FW/AA

Plasmid

#12289

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Mutation

F285A/W286A

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Expression

Plant

Mutation

D156R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Oct4

Plasmid

#19766

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR221 Spinophilin

Plasmid

#87123

Purpose

Gateway donor vector for Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Use

Donor vector

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Expression

Bacterial

Mutation

see manuscript

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g1

Plasmid

#80784

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pME-BASU-NS

Plasmid

#166565

Purpose

Multisite Gateway middle entry clone encoding BASU. Parton lab clone JRO

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g4

Plasmid

#80787

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pKISox1-P2A-eGFP-Hygro

Plasmid

#115685

Purpose

Knock-in of P2A-eGFP at the mouse Sox1 gene

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-eGFP-loxP-PGK-Hygro-loxP

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-NET-myc

Plasmid

#107458

Purpose

Mammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Norepinephrine Transporter (NET) (SLC6A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PsChR2_EYFP

Plasmid

#69057

Purpose

High-efficiency blue-shifted cation channelrhodopsin

Depositor

John Spudich

Article

Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

Insert

PsChR2

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTRANS_232

Plasmid

#91121

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g2

Plasmid

#80785

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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LgBit-NbALFA

Plasmid

#201484

Purpose

NanoBit detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbALFA

Tags

LgBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pME-Ruby2-NS

Plasmid

#166569

Purpose

Multisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Ruby2

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-BASU-EGFP

Plasmid

#163845

Purpose

In vitro transcription of BASU-EGFP. Parton lab clone JRR

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

In vitro transcription of rna

Tags

EGFP

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMV306G13+FFluc

Plasmid

#26157

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Firefly luciferase

Use

Mycobacteria integrating vector

Mutation

Codon optimized for Mycobacterium tuberculosis. …

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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5UAS zfSynaptophysin:GFP

Plasmid

#74316

Purpose

A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

Depositor

Martin Meyer, Stephen Smith

Article

Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

Insert

synaptophysin

Tags

EGFP

Mutation

Q12H and deletion of F13 (please see depositor co…

Promoter

5UAS

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pQTEV-TAGLN2

Plasmid

#31569

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

transgelin 2 (TAGLN2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pBI-p53 wt/EGFP

Plasmid

#16543

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-tdTomato-hphMX6

Plasmid

#105157

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

Insert

tdTomato-hphMX6

Tags

tdTomato

Expression

Bacterial and Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

Plasmid

#19326

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::twk-18(cn110)cDNA

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pFBP_promoter_acceptor

Plasmid

#189920

Purpose

Binary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformation

Depositor

Adam Steinbrenner

Article

Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

Type

Empty backbone

Expression

Plant

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pIntegrase_3

Plasmid

#60575

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_3

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 Set9 wt

Plasmid

#24082

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG and His

Expression

Bacterial

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-H2B_Halo_T2A_EGFP

Plasmid

#135444

Purpose

Mamalian cell expression of nuclear HaloTag7 and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

H2B-HaloTag7 and EGFP

Tags

H2B

Expression

Mammalian

Promoter

CMV/TetO

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA Set9 mutant

Plasmid

#24085

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H297A

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pTRANS_201

Plasmid

#91105

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAFNF-DsRed

Plasmid

#13771

Purpose

Flp recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

DsRed2

Use

Flp/frt

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pCMU-PLAr

Plasmid

#61200

Purpose

Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRubisco1sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 24,865 results for: nes