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164,577 results

Showing: 4841 - 4860 of 164577 results

  3. pCAGGS_3E5E_eGFP

    Plasmid
    #103054
    Purpose
    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Article
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Insert
    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    Use
    Tags
    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    103054-plasmid-map-sequence-id-197128

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  4. SpCas9-NG-ABE8e(V106W)

    Plasmid
    #198533
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198533-plasmid-map-sequence-id-388880

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  11. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  12. pBItetDMPKSGFP

    Plasmid
    #96903
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline responsive and bidirectional
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96903-plasmid-map-sequence-id-193288

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  13. pBItetDT480GFP

    Plasmid
    #96905
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline responsive and bidirectional
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    96905-plasmid-map-sequence-id-306112

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  16. KDM4D

    Plasmid
    #73247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KDM4D (KDM4D Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam02375:JmjN 18-64, pfam02373:JmjC 182-295
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73247-plasmid-map-sequence-id-137202

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  17. PB-CT3G-CERP2-MG

    Plasmid
    #175503
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available Since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175503-plasmid-map-sequence-id-345096

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Showing: 4841 - 4860 of 164577 results