178,736 results
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Plasmid#139098PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingExpressionMammalianPromoterShhAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by
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EF1a_mCherry_P2A_Hygro
Plasmid#135003PurposeLentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ARR3tk-eGFP/SV40-mCherry
Plasmid#132360PurposeTo measure transcriptional activity of Androgen ReceptorDepositorInsertAR responsive elements (AR Human)
UseLentiviralAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)
Plasmid#126580PurposeA repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorInsertOsARF16-PB1 domain
TagsHAExpressionMammalianAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGIPZ-PD-L1-EGFP
Plasmid#120933PurposeExpress PD-L1-EGFP fusion protein in mammalian cellsDepositorInsertPD-L1-EGFP
ExpressionBacterialAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-BRAF-V600E
Plasmid#116204PurposeLentiviral expression of BRAF V600EDepositorAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LbCpf1-2NLS
Plasmid#102566Purposebacterial expression of LbCpf1-2NLSDepositorInsertLbCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-ihSyn1-tTA
Plasmid#99120PurposeAn AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTADepositorHas serviceAAV1InserttTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
ExpressionMammalianAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-Cj-sgRNA
Plasmid#89753PurposeU6 promoter driven expression of Cj-SgRNA cloned with BsmbIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCj-SgRNA
UseSgrna expression under u6 promoterPromoterU6Available sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TrkB mEGFP
Plasmid#83952PurposeFRET donor for dynamically measuring TrkB activity in neuronsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTropomyosin related kinase B (Ntrk2 Mouse)
TagsmEGFPExpressionMammalianAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-NTOM20-LOV2
Plasmid#81009PurposeExpression of LOV2 anchored to mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLOV2
TagsNTOM20ExpressionBacterial, Insect, and Mamm…PromoterCMV, p10Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBluescriptSKII+ U6-sgRNA(F+E) empty
Plasmid#74707PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
ExpressionMammalianAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3 HA C9ORF72 op
Plasmid#74156PurposeExpress HA tagged optimized C9ORF72 in mammalian cellsDepositorAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hNox4
Plasmid#69352Purposeexpresses human Nox4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthNOX4 (NOX4 Human)
ExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-NAT
Plasmid#64329PurposeCas9 expression cassette carried by a yeast single-copy episome plasmidDepositorInsertHuman-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
UseCRISPRExpressionYeastAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by