161,873 results

1. KARS_pNIC-Bio3

   Plasmid

   #153051

   Purpose

   Expression construct for producing Avi tagged Lysyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   KARS (KARS1 Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. ERroGFP_iE_C204Q_pCDNA3

   Plasmid

   #48629

   Purpose

   Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERroGFP
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   insertion E147B, C204Q

   Promoter

   Available since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTagRFP657-N1

   Plasmid

   #31959

   Depositor

   Vladislav Verkhusha

   Article

   Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MOS

   Plasmid

   #64120

   Purpose

   A modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genes

   Depositor

   Linzhao Cheng

   Article

   Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

   Insert

   OCT4-2A-SOX2 (POU5F1, SOX2 Human)
   

   Use

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBFC1171

   Plasmid

   #231994

   Purpose

   CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site

   Depositor

   Brady Cress

   Article

   Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

   Insert

   crRNA of dRfxCas13d
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJEx (Jungle Express)

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-HDAC2

   Plasmid

   #222553

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   HDAC2 (HDAC2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRAU168

   Plasmid

   #86834

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA1

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGDP1 vph

   Plasmid

   #112893

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   vph
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGDP3 vatD

   Plasmid

   #112885

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   vatD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1227 pcDNA3 Myr HA Akt2

    Plasmid

    #9016

    Depositor

    William Sellers

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTT3-ecdCD19-ST3-His_withSUMO

    Plasmid

    #223219

    Purpose

    Expression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expression

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    extracellular domain of B-lymphocyte antigen CD19 (CD19 Human)
    

    Use

    Tags

    Histag, SUMO, and Spytag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CamKII.GCaMP6s.WPRE.SV40

    Plasmid

    #107790

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_C_Myc_DDK_IRES_Puro_TARBP2

    Plasmid

    #106119

    Purpose

    Lentiviral expression of TARBP2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TARBP2 (TARBP2 Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and Myc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti PA-mCit

    Plasmid

    #113451

    Purpose

    Lentiviral ProteinA-mCitrine expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBbC

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-Hygro-GFP

    Plasmid

    #61215

    Purpose

    Retrovirus expressing GFP

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-SynB

    Plasmid

    #174861

    Purpose

    Expresses mouse syncytin B in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    syncytin B (Synb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3E-csGBP

    Plasmid

    #108891

    Purpose

    Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    csGBP
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGDP3 aph(3'')-Ia

    Plasmid

    #112879

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGDP3 aph(9)-Ia

    Plasmid

    #112882

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    aph(9)-Ia
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMXs-Gfi1

    Plasmid

    #158531

    Purpose

    Expresses human Gfi1 in mammalian cells

    Depositor

    Neil Segil

    Article

    Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

    Insert

    Growth Factor Independent Protein 1 (GFI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMVTNT-T7-KPNA4

    Plasmid

    #26680

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 4 (KPNA4 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-AMBRA1-3xFLAG

    Plasmid

    #172605

    Purpose

    Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-NPC1(WT)-FLAG

    Plasmid

    #164972

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIRES proHB-EGF WT

    Plasmid

    #11602

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pMJ826

    Plasmid

    #39316

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Histidine 840 to Alanine (H840A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQF2WB

    Plasmid

    #61312

    Purpose

    Enhancer trap version of QF2, analogous to pGAWB

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NMUR2-Tango

    Plasmid

    #66447

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NMUR2 (NMUR2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pBF3060

    Plasmid

    #26853

    Purpose

    Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Insert

    CreA recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    GAL1

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223-ABL1

    Plasmid

    #23939

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCM184-ParB2::eGFP

    Plasmid

    #87252

    Purpose

    Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB2
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMF-TetTight

    Plasmid

    #193288

    Purpose

    Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    rtTA-P2A-tTS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF‐1α

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. RNF43-SmBiT, BiBiT-Ready Vector

    Plasmid

    #237081

    Purpose

    Express RNF43-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    RNF43 (RNF43 Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. ZNRF3-SmBiT, BiBiT-Ready Vector

    Plasmid

    #237083

    Purpose

    Express ZNRF3-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ZNRF3 (ZNRF3 Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pGloSensor -40F cGMP Vector

    Plasmid

    #236812

    Purpose

    Express -40F cGMP in biosensor assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    -40F
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pGloSensor -42F cGMP Vector

    Plasmid

    #236806

    Purpose

    Express -42F cGMP in biosensor assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    -42F
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. prhaBAD-anti-mouse-Nb-Hia5

    Plasmid

    #239626

    Purpose

    Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

    Insert

    Hia5
    

    Use

    Tags

    6HIS, MBP, TEV, and anti-Mouse Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. prhaBAD-anti-rabbit-Nb-Hia5

    Plasmid

    #239627

    Purpose

    Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

    Insert

    Hia5
    

    Use

    Tags

    6HIS, MBP, TEV, and anti-Rabbit Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HDM_RSV_B_2024_F

    Plasmid

    #237360

    Purpose

    Expression plasmid for RSV B F from 2024

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV B F from 2024 human codon optimized from Genbank Accesion PP660445
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PB-U6-CNCB_sgEIF3D-4

    Plasmid

    #236766

    Purpose

    A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #4 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Insert

    EIF3D sgRNA-4 (EIF3D Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter and mouse U6 promoter, CAG prom…

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge