161,873 results

1. pEBFP2-Nuc

   Plasmid

   #14893

   Purpose

   Mammalian expression of EBFP2 blue fluorescent protein with NLS

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   pEBFP2-Nuc
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Replace EYFP of the vector pEYFP-Nuc from Clontec…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLIX403-ccdB-Blast

   Plasmid

   #158560

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-BCL6-IRES-GFP

   Plasmid

   #31391

   Depositor

   Hilda Ye

   Article

   Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

   Insert

   B-cell lymphoma 6 (BCL6 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

   Plasmid

   #192636

   Purpose

   Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_nMagHigh1-EGFP-CAAX

   Plasmid

   #67300

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   nMagHigh1-EGFP-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI_FLAG-GFP(Y39TAG)-6His

   Plasmid

   #223507

   Purpose

   Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

   Depositor

   Edward Lemke

   Insert

   GFP(Y39TAG)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Y39TAG

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1a-eFFly-eGFP

   Plasmid

   #104834

   Purpose

   eFFly luciferase for invivo Imaging, eGFP for flourescent detection

   Depositor

   Irmela Jeremias

   Article

   Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

   Inserts

   eFFly
   eGFP
   

   Use

   Lentiviral and Luciferase; Egfp

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-dCas13-inactive M3M14nes

   Plasmid

   #157855

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBI-MCS-EGFP

   Plasmid

   #16542

   Depositor

   Bert Vogelstein

   Article

   Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG35

    Plasmid

    #35123

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    natMX3
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-opto-hCaspase-8

    Plasmid

    #208786

    Purpose

    Inducibly expresses human Caspase 8 fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase 8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBlueSKMimRNA

    Plasmid

    #102535

    Purpose

    Template for synthesis of Minos transposase mRNA

    Depositor

    Michalis Averof

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transposase mRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

    Plasmid

    #176704

    Purpose

    neural activity dependent release of sbGLuc from presynaptic vesicles

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

    Inserts

    Gaussia luciferase, slow burn
    dTomato
    

    Use

    AAV

    Tags

    P2A and hPOMC1-26

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mNeptune2-N1

    Plasmid

    #54837

    Purpose

    Localization: N1 Cloning Vector, Excitation: 600, Emission: 650

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mNeptune2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLenti6/V5-p53_R249S

    Plasmid

    #22935

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R249S Mutant

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-p53DD

    Plasmid

    #22729

    Depositor

    Shinya Yamanaka

    Article

    Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 14-301

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-mCherry

    Plasmid

    #193553

    Purpose

    Expresses mEGFP-mCherry in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    mCherry
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2/v5-DEST hGlut4

    Plasmid

    #18087

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut4 (SLC2A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid

    #129631

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLminP_Luc2P_RE3

    Plasmid

    #90337

    Purpose

    AHR - Aryl hydrocarbon receptor gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    AHR

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGDP4 tet(A)

    Plasmid

    #112887

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    tet(A)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Sequence was codon optimized for E. coli K12 usin…

    Promoter

    Plac

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. qTAG-C-mScarlet-EFS-Puro

    Plasmid

    #227259

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAG425GPD-ccdB

    Plasmid

    #14154

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAWP78-PVCpnf_Δpvc10

    Plasmid

    #198448

    Purpose

    Produces PVCs containing truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_Δpvc10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUCP20T-dsRedExpress

    Plasmid

    #78470

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFRT_TO_FlagHA_USP10

    Plasmid

    #127102

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    USP10 (USP10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. araBAD-BCD20-ClpP-BCD17-ClpX

    Plasmid

    #153301

    Purpose

    Expresses clpX and clpP in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Inserts

    clpX (clpX Escherichia coli)
    clpP (clpP Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-EFS-MCP-HALOnls

    Plasmid

    #121937

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    MCP-HALOnls
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBF3060

    Plasmid

    #26853

    Purpose

    Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Insert

    CreA recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    GAL1

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. WT GLUT1-FLAG

    Plasmid

    #200094

    Purpose

    WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET11b-MIF

    Plasmid

    #149446

    Purpose

    Expression of WT MIF in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TetR in pENTR1A (559-1)

    Plasmid

    #22265

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor (tetR )
    

    Use

    Tags

    Expression

    Insect, Mammalian, Worm Exp…

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDH-EF1-IRES-BSD

    Plasmid

    #209751

    Purpose

    Lentiviral transfer plasmid to express the blasticidin resistance gene

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV-Syn-PSD95-mCherry

    Plasmid

    #125694

    Purpose

    Exogenous expression of PSD95 (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Anti-LRP4 (cytoplasmic) [N164/6.1R]

    Plasmid

    #114542

    Purpose

    Mammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA3_cAMPFIRE-H

    Plasmid

    #182281

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-H (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRKH3-slpGFP

    Plasmid

    #27168

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. iF53 PB-Zim3-dCas9-mScarlet-Hygro

    Plasmid

    #204726

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. BASU RaPID plasmid

    Plasmid

    #107250

    Purpose

    Expresses the BASU RaPID labeling protein

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    BASU
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge