162,160 results

1. pWZL hygro Flag HA TRAP220 wt

   Plasmid

   #17433

   Purpose

   Retroviral expression of Med1/TRAP220 in mammalian cells

   Depositor

   Kai Ge

   Article

   Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

   Insert

   TRAP220 (MED1 Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-C3-ZO-2

   Plasmid

   #27422

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pNP1723

   Plasmid

   #222727

   Purpose

   sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-AP-Ub

   Plasmid

   #232577

   Purpose

   Express Ubiquitin tagged with an Acceptor Peptide (AP)

   Depositor

   Sagar Bhogaraju

   Article

   Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

   Insert

   Ubiquitin
   

   Use

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCE-BiFC-VN173

   Plasmid

   #22019

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Nat Protoc. 2008 . 3(4):588-96.

   Insert

   VN173
   

   Use

   Tags

   Myc

   Expression

   Worm

   Mutation

   N/A

   Promoter

   Available since

   Oct. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LentiGuide-TagBFP-2A-BSD

   Plasmid

   #167929

   Purpose

   Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 HA-YY1

   Plasmid

   #104395

   Purpose

   HA-YY1

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   YY1 (YY1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GW1-Mito-pHRed

   Plasmid

   #31474

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   Cox8-tagged pHRed
   

   Use

   Tags

   4xCox8

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHAGE-EF1aL-eGFP-W

   Plasmid

   #126686

   Purpose

   Constitutive, ubiquitous GFP expression.

   Depositor

   Darrell Kotton

   Article

   Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1aL

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLgw V5-EcoDam

    Plasmid

    #59210

    Purpose

    Mammalian DamID control lentiviral vector for expression of V5-Dam only

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Insert

    EcoDam
    

    Use

    Lentiviral; Damid

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter and heat shock minima…

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-FLAG-DDB1

    Plasmid

    #19918

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.3_OCT4

    Plasmid

    #26816

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-OCT4-3'UTR (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pBbA5a-sufABCDSE

    Plasmid

    #195056

    Purpose

    Expresses SUF operon from E. coli from IPTG-inducible promoter

    Depositor

    Greg Bokinsky

    Article

    D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

    Insert

    sufABCDSE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GST-mDia1(FH1FH2DAD)-His

    Plasmid

    #85822

    Purpose

    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.

    Depositor

    Guillaume Romet-Lemonne, Antoine Jégou

    Article

    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc

    Insert

    Protein diaphanous homolog 1 (Diaph1 Mouse)
    

    Use

    Tags

    8*His and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-BE1

    Plasmid

    #73019

    Purpose

    Expresses BE1 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZJOM90

    Plasmid

    #133647

    Purpose

    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-HSF1

    Plasmid

    #32537

    Depositor

    Stuart Calderwood

    Article

    Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    CMV

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 human cofilin S3E

    Plasmid

    #50855

    Purpose

    expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3E (CFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S3E psuedo-phosporylated, non-activatable

    Promoter

    CMV

    Available since

    Feb. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

    Plasmid

    #198292

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p426*-SpSgH

    Plasmid

    #136993

    Purpose

    Helper plasmid for SpCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV 3xFLAG-LC3B WT

    Plasmid

    #123092

    Purpose

    Expresses 3xFLAG-LC3B wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLVX-NPC1(WT)-FLAG

    Plasmid

    #164972

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET32a-HD46Q

    Plasmid

    #11515

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 46Q glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 46 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YFP-Parkin

    Plasmid

    #23955

    Purpose

    Mammalian expression of human Park2 fused to EYFP

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Park2 (PRKN Human)
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pScCas9

    Plasmid

    #117700

    Purpose

    Mammalian expression vector encoding WT ScCas9

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

    Insert

    Streptococcus canis Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIRES2-EGFP Mer

    Plasmid

    #14998

    Depositor

    Raymond Birge

    Article

    Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53

    Insert

    Mer (Mertk Mouse)
    

    Use

    Tags

    IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223-FES

    Plasmid

    #23876

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FES (FES Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

28. DRD2-Tango

    Plasmid

    #66269

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    DRD2 (DRD2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mEmerald-Claudin7-C-12

    Plasmid

    #54041

    Purpose

    Localization: Tight Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Claudin7 (CLDN7 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pDZ416 (24PP7SL loxP-Kan-loxP)

    Plasmid

    #45163

    Depositor

    Daniel Zenklusen, Robert Singer

    Article

    Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

    Insert

    24xPP7 Stem Loop Cassette
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

    Plasmid

    #100157

    Purpose

    Expresses MUS81

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    MUS81 (MUS81 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCX-ThPDE5VV

    Plasmid

    #134778

    Purpose

    cGMP FRET biosensor

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    ThPDE5VV
    

    Use

    Tags

    cpVenus-Venus and mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET21-Streptavidin-K121R

    Plasmid

    #89880

    Purpose

    Streptavidin that has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K121R-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K121R

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HIVGKO

    Plasmid

    #112234

    Purpose

    HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

    Depositor

    Eric Verdin

    Article

    Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

    Insert

    csGFP-EF1a-mKO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-ERBB4

    Plasmid

    #29527

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

    Plasmid

    #188383

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Flag-HA-USP21

    Plasmid

    #22574

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP21 (USP21 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Str-Golgin84_VSVG-SBP-EGFP

    Plasmid

    #65305

    Purpose

    synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJAM1.19_PB-CIT-PGK-Blast

    Plasmid

    #205564

    Purpose

    Express human CIT

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    CIT (CIT Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMSCV PIG (Puro IRES GFP empty vector)

    Plasmid

    #21654

    Purpose

    Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge