159,258 results

1. FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1

   Plasmid

   #58434

   Purpose

   Induces Citrine expression from a promoter containing 4 lexA boxes in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)4-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRCC-K

   Plasmid

   #81191

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOZ-N-FH KIAA0157

   Plasmid

   #27499

   Depositor

   Roger Greenberg

   Article

   Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

   Insert

   KIAA0157 (ABRAXAS2 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCX-DI-2A

   Plasmid

   #74671

   Purpose

   Mammalian expression of DI-2A (hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

   Inserts

   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Use

   Tags

   F2A

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5-hPPT1

   Plasmid

   #85815

   Purpose

   Expression of hPPT in mammalian cells

   Depositor

   Sandra Hofmann

   Article

   Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

   Insert

   human PPT1 (PPT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flamindo2

   Plasmid

   #73938

   Purpose

   Expresses biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   Flamindo2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

   Plasmid

   #211471

   Purpose

   lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   Cas9_T2A_mNeonGreen_P2A_BlasticidinR
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF-1a

   Available since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. eGFP-ova-C1

    Plasmid

    #163524

    Purpose

    Expresses both eGFP and chicken ovalbumin in mammalian cells

    Depositor

    Thomas Weber

    Insert

    enhanced-GFP-chicken ovalbumin tandem
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV3Tag8li-hHP1BP3-HA

    Plasmid

    #165468

    Purpose

    Human HP1BP3 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-mNeonGreen

    Plasmid

    #99134

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a-SUMO3

    Plasmid

    #53143

    Purpose

    E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    SUMO3 (SUMO2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-93)

    Promoter

    T7

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. tdTomato-C1

    Plasmid

    #54653

    Purpose

    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAX2

    Plasmid

    #117398

    Purpose

    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF0832

    Plasmid

    #141725

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GTF2F1 (GTF2F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLAMP1-miRFP703

    Plasmid

    #79998

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-mPCSK3

    Plasmid

    #122674

    Purpose

    Expresses mouse PCSK3 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lamin A (G465D)-mRFP

    Plasmid

    #124271

    Purpose

    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (G465D) (LMNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (G465D)

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSV Sport RXR alpha

    Plasmid

    #8882

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    RXR alpha (RXRA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pITGA4-GFP

    Plasmid

    #81178

    Purpose

    Mammalian expression of human full length ITGA4-EGFP

    Depositor

    Chinten James Lim

    Insert

    ITGA4 (ITGA4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains KLGFFKR motif; A1002L (please see deposi…

    Promoter

    CMV

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. opto-RhoA-mCherry_pcDNA3.1

    Plasmid

    #164472

    Purpose

    Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

    Insert

    opto-RhoA
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL3-mArg1 promoter/enhancer -31/-3810

    Plasmid

    #34571

    Depositor

    Peter Murray

    Article

    Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

    Insert

    Arg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pF CAG luc puro

    Plasmid

    #67501

    Purpose

    Constitutive expression of firefly luciferase in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pQTEV-PTD004

    Plasmid

    #34798

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    GTP-binding protein PTD004 (OLA1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG/ATP9A(E195Q)-HA

    Plasmid

    #209244

    Purpose

    Mammalian expression of ATP9A mutant E195Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E195Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. B-GRAM-H

    Plasmid

    #211706

    Purpose

    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223_PIK3CA_p.E545K

    Plasmid

    #82881

    Purpose

    Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, Jesse Boehm, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    NM_006218 (PIK3CA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E545K

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL3-NurRE3

    Plasmid

    #208253

    Purpose

    pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

    Depositor

    Douglas Kojetin

    Article

    Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

    Insert

    3X Nur response element LUC
    

    Use

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. tdTomato-pBAD

    Plasmid

    #54856

    Purpose

    Localization: Protein Expression Vector , Excitation: 554, Emission: 581

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Type

    Empty backbone

    Use

    Tags

    6xHis and tdTomato

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TMEM106B-EGFP

    Plasmid

    #210366

    Purpose

    Expresses TMEM106B fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    TMEM106B (TMEM106B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV5B-ALK4

    Plasmid

    #11756

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    ALK4 (ACVR1B Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

33. pYDS2.0_AT118-H

    Plasmid

    #222846

    Purpose

    Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HPV16 E6 TCR

    Plasmid

    #122727

    Purpose

    Express HPV16 E6 TCR

    Depositor

    Christian Hinrichs

    Article

    Draper et al Clin Cancer Res. 2015 Oct 1;21(19):4431-

    Insert

    HPV16 E6 TCR
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMetYC-Dest

    Plasmid

    #105081

    Purpose

    Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Use

    Tags

    Cub-PLV

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAVS1-iPE-Plus

    Plasmid

    #214062

    Purpose

    inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Flag-FOXM1

    Plasmid

    #153136

    Purpose

    Expression of Flag tagged human FOXM1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXM1 (FOXM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFX-mito-mKate2

    Plasmid

    #174178

    Purpose

    Express mKate2 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mKate2 with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. c-Myc-5-HT2A

    Plasmid

    #67944

    Purpose

    N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

    Depositor

    Javier Gonzalez-Maeso

    Article

    Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ART

    Plasmid

    #214063

    Purpose

    Fluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge