164,425 results

1. BCL2L1_pLX307

   Plasmid

   #98323

   Purpose

   Lentiviral expression of BCL2L1

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiRA1-MYB

   Plasmid

   #141120

   Purpose

   Lentiviral overexpression of MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   MYB (MYB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LPAR4-Tango

   Plasmid

   #66420

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   LPA4 (LPAR4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLX-B2 (GB3374)

   Plasmid

   #160636

   Purpose

   pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

   Depositor

   Juan Antonio García, Fabio Pasin

   Article

   Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HiFi-nCas9-NG_P2A-mCherry

   Plasmid

   #219936

   Purpose

   Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
   

   Use

   CRISPR

   Tags

   SV40 bpNLS and SV40 bpNLS-P2A-mCherry

   Expression

   Mammalian

   Mutation

   HiFi-nCas9(D10A/R691A)

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mApple-Sec61b-C1

   Plasmid

   #90993

   Purpose

   Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mApple-Sec61b (SEC61B Synthetic, Human)
   

   Use

   Tags

   mApple (red fluorescence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-dSa-VPR mini.-syn pA

   Plasmid

   #99689

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6691 Bicistronic_ires_puro

   Plasmid

   #64335

   Purpose

   This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-mTagBFP2-PLCδ-PH

   Plasmid

   #214275

   Purpose

   Mammalian expression of blue fluorescent PI(4,5)P2 reporter mTagBFP2-PLCδ-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   mTagBFP2-PLCδ(1-175) (PLCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE5max-SF3B1-K700E

    Plasmid

    #200317

    Purpose

    Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Inserts

    K700E-ng1+pg2
    PEmax-2A-MLH1dn
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-ER-mRFP

    Plasmid

    #231176

    Purpose

    Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cells

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    ER-mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLO-GFP-1UAG

    Plasmid

    #82500

    Purpose

    Expresses GFP with 1 UAG codon at the amino acid position 3

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescent Protein with 1 UAG codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3

    Promoter

    AraBAD

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUCmini-iCAP-PHP.AX

    Plasmid

    #195218

    Purpose

    AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

    Depositor

    Viviana Gradinaru

    Article

    Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

    Insert

    Synthetic construct isolate AAV-PHP.AX VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIP-CMV-GFP-FLAG-cGAS

    Plasmid

    #86675

    Purpose

    Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-ZFN-AAVS1

    Plasmid

    #89707

    Purpose

    Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

    Insert

    ZFN-AAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mRFP-Ub

    Plasmid

    #11935

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin (RBX1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-His3MX6-PGAL1

    Plasmid

    #41607

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-AcGFP1-ORP9-PH

    Plasmid

    #214266

    Purpose

    Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    AcGFP1-ORP9(1-111) (Osbpl9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAGK111

    Plasmid

    #228126

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. qTAG-C-miniTurbo-Puro

    Plasmid

    #207725

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pGR595

    Plasmid

    #213785

    Purpose

    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDisplay-GRAB_g5-HT3.0

    Plasmid

    #208711

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR1A no ccDB (w48-1)

    Plasmid

    #17398

    Purpose

    Gateway entry 1A vector, no ccDB gene

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. CL20_mEGFP_EWSR1_WT1

    Plasmid

    #205820

    Purpose

    Express mEGFP-tagged fusion protein, EWSR1_WT1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    EWSR1_WT1 (EWSR1, WT1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EBFP2-Nucleus-7

    Plasmid

    #55249

    Purpose

    Localization: Nuclear Localization, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Use

    Tags

    EBFP2-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

28. Str-Ii_SBP-mCherry-Golgin84

    Plasmid

    #65304

    Purpose

    synchronize trafficking of Golgin-84 from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p3xFLAG-CMV10-mApg16L

    Plasmid

    #24302

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

    Insert

    autophagy related genes 16L (Atg16l1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V583M

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGIG_V5-PPP6C

    Plasmid

    #92013

    Purpose

    Expresses V5-PPP6C, includes IRES-Emerald

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SETD2-GFP

    Plasmid

    #80653

    Purpose

    mammalian expression of SETD2-GFP

    Depositor

    Sérgio De Almeida

    Article

    Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

    Insert

    SETD2 (SETD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. p3XFlag-CMV10-Flag-mMcl-1

    Plasmid

    #32978

    Depositor

    Joseph Opferman

    Article

    Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

    Insert

    Mcl-1 (Mcl1 Mouse)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CMV-mGFP-pA(AV222)

    Plasmid

    #117858

    Purpose

    This plasmid expresses membrane anchored GFP to facility axon morphology study.

    Depositor

    C. Ron Yu

    Article

    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

    Insert

    mGFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pVS1-VIR2

    Plasmid

    #134745

    Purpose

    Virulence and replication helper

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    vir gene cluster
    

    Use

    Vir gene expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pK170.AAV-TRE-Cre-WPRE (Supernova)

    Plasmid

    #85040

    Purpose

    For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nlsCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFRT-TODestGFP_RNAseH1

    Plasmid

    #65784

    Purpose

    expresses GFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Zebrafish, Human)
    

    Use

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SIRT2 Flag

    Plasmid

    #13813

    Purpose

    Mammalian expression of human SIRT2 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Transcript variant 2

    Promoter

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TRE-mCherry-p2A-Chrimson-TS

    Plasmid

    #158702

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    TRE-mcherry-p2A-Chrimson-TS
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGal4x5-E4T-G210

    Plasmid

    #171077

    Purpose

    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4-E4-Gless-cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP_CD47_LU

    Plasmid

    #65474

    Purpose

    GFP tagged CD47 with its own long 3'UTR

    Depositor

    Christine Mayr

    Article

    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

    Inserts

    Signal peptide of CD47
    GFP
    CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge