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Showing: 751 - 780 of 943 results

  1. EcSTH-LbNOX

    Plasmid
    #211924
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.
    Depositor
    Binghui Li
    Article
    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:
    Insert
    EcSTH-LbNOX
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    Available since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    211924-plasmid-map-sequence-id-419891

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  2. CROPseq-Puro-24xMS2

    Plasmid
    #212629
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212629-plasmid-map-sequence-id-420222

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  7. CROPseq-Puro-2xMS2

    Plasmid
    #212627
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212627-plasmid-map-sequence-id-420278

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  8. CROPseq-Puro-12xMS2

    Plasmid
    #212628
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212628-plasmid-map-sequence-id-422312

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  9. CROPseq-Puro-4xMS2

    Plasmid
    #212626
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212626-plasmid-map-sequence-id-420274

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  10. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    90086-plasmid-map-sequence-id-182687

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  11. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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  18. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155046-plasmid-map-sequence-id-308670

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  19. plenti-As-Cas12a-2xNLS

    Plasmid
    #155047
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    AsCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155047-plasmid-map-sequence-id-308054

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  28. pLCHKOv3-mClover-intergenic_2-As

    Plasmid
    #218814
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218814-plasmid-map-sequence-id-436107

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  29. pLCHKOv3-mClover-intergenic_3-As

    Plasmid
    #218815
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218815-plasmid-map-sequence-id-436108

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  30. pLCHKOv3-mClover-intergenic_4-As

    Plasmid
    #218816
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218816-plasmid-map-sequence-id-436109

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Showing: 751 - 780 of 943 results