We narrowed to 11,832 results for: NSI;
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pGG-GFP-PM-TurboID
Plasmid#209402PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in plantaDepositorInsertcTP-3XHA-TurboID
TagsGFPExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM5080
Plasmid#85106PurposePtrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expresDepositorInsertYFP-KaiC
ExpressionBacterialAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-GPXC71S
Plasmid#191760PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758DepositorInsertnuc-roGFP2-GPXC71S
UseBinary agrobacterium ti plasmidExpressionPlantMutationGPX has no peroxidatic Cysteine 71, which is repl…PromoterCaMV 35SAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyn-CFP-GAI(1-92)
Plasmid#37311DepositorInsertGAI (GAI Mustard Weed)
TagsCFP and LynExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable SinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1ExpressionYeastAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-RapACR-EYFP
Plasmid#111075PurposeFast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spikingDepositorInsertAnion channelrhodopsin RapACR
UseLentiviralTagsEYFPAvailable SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV Gab1(PRD)-mCherry-HOTag3
Plasmid#190026PurposeTransiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.DepositorInsertGab1(PRD) CluMPS
ExpressionMammalianPromoterCMVAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAL190
Plasmid#22279PurposeExpresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitmentDepositorInsertTimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
UseSynthetic BiologyExpressionMammalianPromoterSV40Available SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C-SLC35B2
Plasmid#155037PurposeTransient expression of EGFP-SLC35B2DepositorAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCph8
Plasmid#22869DepositorInsertpCph8
UseSynthetic BiologyExpressionBacterialMutationFirst 517 amino acids of Cph1 and the last 229 am…Available SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEPQD2KN0897
Plasmid#177090PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatBDepositorInsertCrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-jRCaMP1b
Plasmid#191468PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent proteinDepositorInsertjRCaMP1b
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-GAI(1-92)
Plasmid#37317DepositorAvailable SinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA
Plasmid#97010PurposeRosa26 targeting vector for FLP-responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCer-Vamp2(qv/fw)
Plasmid#53238PurposeMutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
Tags4aa-linker and mCeruleanExpressionMammalianMutationMutated Q76 to V; F77 to W.Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
CFP-GAI(1-532)
Plasmid#37310DepositorAvailable SinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
gCOTS-pyl
Plasmid#92048PurposeThis is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.DepositorInsertsPyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseCyanobacteria s. elongatus genome recombination i…PromoterLeuP (native S. elongatus promoter) and PrcbLAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID
Plasmid#209393PurposeTransiently expressing TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV/MASC_(GLuc)_INT
Plasmid#68440PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXb3
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS
Plasmid#63170PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporterDepositorInsert"spaghetti monster GFP-OLLAS"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP
Plasmid#185687PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).DepositorInsertERmembrane-GST-2xHaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-TREX-GFP-FF3
Plasmid#11667Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianAvailable SinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pU1/Sm/3' Box_(GLuc)_INT
Plasmid#68438PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U1Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-GPXC71S
Plasmid#191703PurposeTi plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701DepositorInsertchl-redox(ro)GFP2-GPXC71S
UseBinary agrobacterium ti plasmidExpressionPlantMutationPeroxidatic Cysteine 71 replaced with SerinePromoterCaMV 35SAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[P4-P6(3xPP7)]
Plasmid#68432PurposeTransient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_T7_INT[6xminimal_Puf1]
Plasmid#107608PurposeTransient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoterDepositorInsert6x minimal Puf1 motif
UseSynthetic BiologyExpressionMammalianPromoterU6Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SLC7A11 sh926R
Plasmid#118701Purposeentry clone for SLC7A11 insensitive to hairpin TRCN0000288926DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_4
Plasmid#78208PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak bindingDepositorInsertmatB (His6 C106A R286C T303A Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A R286C T303A Q457C K488APromoterT7Available SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_3
Plasmid#78207PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak bindingDepositorInsertmatB (His6 C106A T167A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A T167A R286C Q457C K488APromoterT7Available SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger3-TS-EYFP
Plasmid#127241PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger3-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterCamKIIaAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0121
Plasmid#177053PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoterDepositorInsertiridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0117
Plasmid#177049PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoterDepositorInsertiridoid synthase (CrISY) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-eGFP-NT1-6-gix-6-NT1
Plasmid#81204Purposecontains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmidDepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
ExpressionMammalianPromoterpCBaAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-Sesn1b
Plasmid#61867PurposeTransient overexpression of Flag-Sesn1b (mouse)DepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only
