159,371 results
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pCDB179-DuraPETase
Plasmid#218699PurposeEncodes codon optimized DuraPETase enzyme for bacterial expressionDepositorInsertDuraPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAX2
Plasmid#117398Purposeallelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switchDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A Q67L
Plasmid#28049DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationQ67LPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vinculin-23
Plasmid#55159PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertVinculin (VCL Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
G13 sensor (Ga13_2)
Plasmid#112930PurposeA FRET sensor for G13 activation, encoded on a single plasmid.DepositorInsertG13 sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_alphaTubulin_C1
Plasmid#85045PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorInsertAlpha-Tubulin (TUBA1B Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB
Plasmid#166145PurposeGateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.DepositorTypeEmpty backboneUseTagsHis10ExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
IFI16-FL-(1-729)
Plasmid#35051DepositorInsertIFI16 (IFI16 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
ARTi
Plasmid#214064PurposeFluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pepper-fused sgRNA
Plasmid#217520PurposeExpresses Pepper-fused sgRNA in mammalian cells with U6 promoterDepositorInsertPepper-fused sgRNA for spCas9
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P13
Plasmid#120024PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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mEos4b-CenpA-C-22
Plasmid#62775PurposeLocalization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581DepositorInsertCenpA (CENPA Human)
UseTagsmEos4bExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-PSDΔ1,2-FRBx3
Plasmid#225827PurposeA component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)DepositorInsertPSDΔ1,2-FRBx3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
KRphi-mRFP
Plasmid#17276DepositorInsertKR phi
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-EBFP-tTAn
Plasmid#172122PurposeConstitutive expression of EBFP and rtTA-InteinNDepositorInsertEBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
medSIIN
Plasmid#185664PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
UseLentiviralTagsExpressionMutationPromoterEf1aAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ubiquitin
Plasmid#187910PurposeMammalian cell expression of EGFP-tagged UbiquitinDepositorInsertUBB (UBB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mgSrtA
Plasmid#125792PurposeTransfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.DepositorInsertmgSrtA
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
p415-PtrpC-Cas9-TtrpC-CYC1t
Plasmid#68059PurposeExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.DepositorInserthumanized Cas9
UseCRISPR; N. crassa, fungiTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-TAX1BP1
Plasmid#171423PurposeFor the purifcation of TAX1BP1 proteinDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_class_I_2
Plasmid#164987PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genesDepositorInsertgRNA against HLA class I
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry
Plasmid#159655PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY108 (lenti-AsCpf1)
Plasmid#84739PurposeLenti virus delivery of AsCpf1 and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
UseLentiviralTags3xHA, NLS, and P2AExpressionMutationPromoterEFSAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP
Plasmid#91975PurposeEGFP reporter cell line control (no miR-19 sites)DepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-H1-10
Plasmid#57457PurposeLocalization: Nucleus/Histones, Excitation: 507 / 572, Emission: 516 / 580DepositorInsertH1 (H1f0 Mouse)
UseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTFP1-N1
Plasmid#54521PurposeLocalization: N1 Cloning Vector, Excitation: 462, Emission: 492DepositorTypeEmpty backboneUseTagsmTFP1ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF.myc.ER-E2-Crimson
Plasmid#38770PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertE2-Crimson
UseTagsER retention signal (KDEL), ER signal sequence (M…ExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
SMARCA2-GFP
Plasmid#65390PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSMARCA2 (SMARCA2 Human)
UseTagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
VPR-ps-dSpCas9
Plasmid#102855PurposeA single-chain light-controllable dSpCas9 with pdDronpa1.2 domainsDepositorInsertdSpCas9, pdDronpa1.2
UseCRISPR and Synthetic BiologyTagsNLS and VP64-p64-Rta transcription activatorExpressionMammalianMutationPromoterPGKAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA EED wt
Plasmid#24231PurposeMammalian expression of human EED with HA tagDepositorInsertEED (EED Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFKB-TA-LUC-UBC-dTomato-W
Plasmid#49335PurposepHAGE NFKB-TA-LUC-UBC-dTomato-WDepositorInsertsNFkB consensus binding sequence
TA
Luciferase
UBC
dTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB-mTSPAN4-GFP
Plasmid#210329PurposeOverexpression of mTSPAN4DepositorInsertmTSPAN4 (Tspan4 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-CD9-10
Plasmid#57705PurposeLocalization: Tetraspanin/Membrane, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertCD9 (CD9 Human)
UseTagsDendra2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-mAirn-R-loop
Plasmid#134900PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInsertmAirn promoter (Airn Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-MCL1
Plasmid#21605DepositorInsertMCL-1 (MCL1 Human)
UseTagsExpressionMammalianMutationInsert ends with a STOP codon, no V5-His-tag.PromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCI-Caspase 1
Plasmid#41552DepositorInsertCaspase-1 (CASP1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMV immediate-early enhancer/promoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only
