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Showing: 1301 - 1350 of 17132 results

  4. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84386-plasmid-map-sequence-id-272895

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  17. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid
    #229680
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    SNAP, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229680-plasmid-map-sequence-id-457075

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  18. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Andrea Németh
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    101039-plasmid-map-sequence-id-190363

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  21. LentiCROP-PuroR-P2A-GFP-U6

    Plasmid
    #200029
    Purpose
    Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    PuroR-P2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200029-plasmid-map-sequence-id-393200

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  22. qTAG-C-mNeon-Puro-TOMM20

    Plasmid
    #207790
    Purpose
    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    207790-plasmid-map-sequence-id-415639

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  26. pTwist-SFFV-NFE2L18ND-Puro

    Plasmid
    #181918
    Purpose
    Lentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.
    Depositor
    Scott Dixon
    Article
    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11
    Insert
    NFE2L1 (NFE2L1 Human)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    8 putative N-glycoyslation sites converted from a…
    Promoter
    SFFV
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181918-plasmid-map-sequence-id-358683

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  41. qTAG-AAVS1-PGK-Puro

    Plasmid
    #227269
    Purpose
    Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227269-plasmid-map-sequence-id-452020

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  45. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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  46. pBabe puro IRES-EGFP

    Plasmid
    #14430
    Depositor
    L. Miguel Martins
    Has Service
    Cloning Grade DNA
    Insert
    IRES-EGFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    14430-plasmid-map-sequence-id-251302

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  48. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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Showing: 1301 - 1350 of 17132 results