We narrowed to 30,434 results for: uros

1. pME-mKOFP2-CAAX

   Plasmid

   #75155

   Purpose

   Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mKOFP2-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSicoR human Drosha3

   Plasmid

   #14768

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 Apr 1. ():.

   Insert

   Drosha shRNA (DROSHA Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pNEXdelta-ApAF

   Plasmid

   #13334

   Depositor

   Bong-Kiun Kaang

   Article

   Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

   Insert

   Aplysia activating factor
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pMaCTag-P24

   Plasmid

   #120035

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   TAP
   

   Use

   Pcr template

   Tags

   TAP

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lyn-tdnano-HA

   Plasmid

   #136512

   Purpose

   Mammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag

   Depositor

   Bianxiao Cui

   Article

   Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

   Insert

   Lyn nano nano 3HA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSicoR mouse Dicer3

   Plasmid

   #14781

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 Apr 1. ():.

   Insert

   Dicer shRNA (Dicer1 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pMaCTag-P10

   Plasmid

   #120021

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mKOk
   

   Use

   Pcr template

   Tags

   mKOk

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pbs/pU6-HDAC4 RNAi

   Plasmid

   #21100

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   RNAi against histone deacetylase4 (HDAC4 Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pBSc + MShab

   Plasmid

   #16146

   Depositor

   Larry Salkoff

   Article

   Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

   Insert

   Mus musculus potassium voltage gated channel (Kcnb1 Mouse, Fly)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pBABE flag M33

    Plasmid

    #1951

    Depositor

    Robert Kingston

    Article

    Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pMSCV-FlagBcl10K31RK63R

    Plasmid

    #18719

    Depositor

    Jon Ashwell

    Article

    Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

    Insert

    B cell lymphoma 10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Lysine 31 to arginine and Lysine 63 to arginine.

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pLib6.6

    Plasmid

    #176652

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FH95pUp95GW (B4)

    Plasmid

    #74013

    Purpose

    lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMaCTag-P18

    Plasmid

    #120029

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. fADL-1e-flag-linker-p3

    Plasmid

    #139441

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, flag-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L1-A426D

    Plasmid

    #15485

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-A426D (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 426 changed to Aspartic Acid

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-fCAMK-GFP

    Plasmid

    #22917

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    fugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AV11_pDonor.EP.S1

    Plasmid

    #100296

    Purpose

    AAVS1-targeting expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    PuroR.T2A.EGFP expression unit
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAPM D4 BsmBI Myd88 stuffer

    Plasmid

    #128505

    Purpose

    pAPM knockdown vector with Myd88 stuffer sequence

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    Puromycin Resistance
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. APneg-VAMP1d

    Plasmid

    #168010

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGAD424-hSyntenin2b

    Plasmid

    #89427

    Purpose

    Two-hybrid system

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Use

    Tags

    Gal4 AD

    Expression

    Yeast

    Mutation

    *see below

    Promoter

    ADH1

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAPM-D4-miR30-TRIM5

    Plasmid

    #132932

    Purpose

    Lentivector for TRIM5 knockdown and puromycin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    TRIM5 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cenp-B DBD INCENP mCherry

    Plasmid

    #45233

    Purpose

    Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B-DBD INCENP-Δcen mCherry
    

    Use

    Tags

    DNA binding domain of CENP-B and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLib6.4B

    Plasmid

    #176665

    Purpose

    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMaCTag-P17

    Plasmid

    #120028

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mEos4b
    

    Use

    Pcr template

    Tags

    mEos4b

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLOT-syb-sp-YFP1-10

    Plasmid

    #73700

    Purpose

    Neuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASP

    Depositor

    Marco Gallio

    Article

    Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

    Insert

    Neuronal-synaptobrevin:spYFP1–10 chimera
    

    Use

    Lexaop reporter vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 3486 pBABE Bid p22 GFP

    Plasmid

    #8777

    Depositor

    Stanley Korsmeyer

    Article

    Zha et al Science 2000 Dec 1;290(5497):1761-5.

    Insert

    Bid p22 (Bid Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

28. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Halo-WIPI2B R108E

    Plasmid

    #176004

    Purpose

    Expresses WIPI2B (R108E) fused to Halo in mammalian cells

    Depositor

    Erika Holzbaur

    Article

    Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

    Insert

    WIPI2B (R108E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Arginine 108 to Glutamate

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHA-ΔN332-Kin1C

    Plasmid

    #31608

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN332 kinesin-1C (Kif5c Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

    Plasmid

    #128166

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRL1124

    Plasmid

    #70690

    Purpose

    provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Inserts

    avaiM
    eco47iiM
    ecot22iM
    

    Use

    Bacterial shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1 FLIPE-10u

    Plasmid

    #13542

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207R in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDisplay FLIPE-1m

    Plasmid

    #13548

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTK-EdnrbE2-Citrine

    Plasmid

    #127770

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFU-cMVIIA-PE

    Plasmid

    #24147

    Depositor

    Ines Ibanez-Tallon

    Article

    Auer et al Nat Methods. 2010 Feb 7. ():.

    Insert

    loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Conus magus, Aequorea victoria, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p3E-EGFP-NS-MCS

    Plasmid

    #75180

    Purpose

    EGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    EGFP-NS-MCS
    

    Use

    Three prime entry vector for multisite gateway th…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDisplay FLIPE-10u

    Plasmid

    #13546

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207R in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Double UP mNeon to mScarlet P2A Rac1-wt

    Plasmid

    #125135

    Purpose

    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged WT Rac1.

    Depositor

    Erik Dent

    Article

    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

    Insert

    mNeon/mScarletP2A-Rac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Removed Not1 site in mScarlet

    Promoter

    CAG

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pME18S-3HA-mULK2

    Plasmid

    #22898

    Depositor

    Masaaki Muramatsu

    Article

    Yan et al Oncogene. 1999 Oct 21. 18(43):5850-9.

    Insert

    UNC-51-like kinase 2 (Ulk2 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only