163,606 results

1. CYFIP1.mCherry

   Plasmid

   #122051

   Purpose

   Expresses human CYFIP1 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP1 (CYFIP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAVS1-P-CAG-GFP

   Plasmid

   #80491

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   CAG-GFP
   

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmCherry-MS2coatProtein

   Plasmid

   #103832

   Purpose

   Live cell reader for MS2 loop RNA production

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   mCherry-MS2 coat protein (cp )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiCRISPRi(v2)-Blast

   Plasmid

   #170068

   Purpose

   Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-FLEX-GFP

   Plasmid

   #28304

   Depositor

   Edward Boyden

   Insert

   GFP
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRPML1-G-GECO1.2-ERES

   Plasmid

   #207144

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Mcoln1 (MCOLN1 Human)
   

   Use

   Tags

   ER-Export Signal (ERES) and G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EF1a_Puro_Telo_v2

   Plasmid

   #195139

   Purpose

   ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Telomere (RTEL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-pmExRai-AKAR2

   Plasmid

   #161755

   Purpose

   Plasma membrane-targeted ExRai-AKAR2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   Lyn-ExRai-AKAR2
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGFP-Cytochrome C

   Plasmid

   #41181

   Depositor

   Douglas Green

   Article

   Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

   Insert

   Cytochrome C (CYCS Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-dCas12a-ABE8e

    Plasmid

    #193645

    Purpose

    Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHybrid-Electra2

    Plasmid

    #179475

    Purpose

    Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Inserts

    Electra2
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PdnaK GFP pZa

    Plasmid

    #170073

    Purpose

    GFP expression under the control of E. coli dnaK promoter

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PdnaK

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLminP_Luc2P_RE17

    Plasmid

    #90359

    Purpose

    NFAT - PKC/Ca++ gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFAT

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3_NSlmb-vhhGFP4

    Plasmid

    #35579

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSlmb-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.2/DEST/hTfR-HA

    Plasmid

    #69610

    Purpose

    human transferrin receptor with an HA tag fused at the C-terminus

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    human transferrin receptor (TFRC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEVOL-pylT-N346A/C348A

    Plasmid

    #127411

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N346A-C348A

    Promoter

    araBAD

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLT3-D835Y-MSCV-IRES-GFP

    Plasmid

    #184780

    Purpose

    Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Activating tyrosine kinase domain mutation (D835Y)

    Promoter

    MSCV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-STK11

    Plasmid

    #116794

    Purpose

    Lentiviral expression of STK11

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    STK11 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2

    Plasmid

    #185449

    Purpose

    Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TRF2 (TERF2 Human)
    RAP1 (TERF2IP Human)
    

    Use

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FUW-OSKM

    Plasmid

    #20328

    Purpose

    Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSin Capsid

    Plasmid

    #127693

    Purpose

    Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Capsid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

    Plasmid

    #194681

    Purpose

    CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 6 x DBE-luc

    Plasmid

    #153160

    Purpose

    Luciferase reporter plasmid for FOXTF

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid

    #128605

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mApple-Sec61b-C1

    Plasmid

    #90993

    Purpose

    Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mApple-Sec61b (SEC61B Human, Synthetic)
    

    Use

    Tags

    mApple (red fluorescence)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBR_attB(bxb)_ccdB_lox_v2

    Plasmid

    #198040

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry-PARP1

    Plasmid

    #215136

    Purpose

    Expresses mCherry-PARP1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

    Plasmid

    #191795

    Purpose

    vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    A134V, A140V, A141V, A143V

    Promoter

    EFS, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti6/V5-p53_R280K

    Plasmid

    #22933

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    R280K mutant

    Promoter

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only