161,805 results

1. pCXLE-gw

   Plasmid

   #37626

   Purpose

   Non-integrating (episomal) expression vector with Gateway cassette

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. TERT - WT

   Plasmid

   #213827

   Purpose

   Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   human TERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC18T-mini-Tn7T-Zeo-gfpmut3

   Plasmid

   #65037

   Purpose

   ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Use

   Tags

   GFP11x7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Use

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

   Plasmid

   #198292

   Purpose

   Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-antiMouseMHCIINb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-myc3-CUL4B

   Plasmid

   #19922

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   Cullin 4B (CUL4B Human)
   

   Use

   Tags

   3xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5_T7-GAP43-mScarlet3

   Plasmid

   #225932

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   GAP43-mScarlet3
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_EF1a_TauFRET_3xKQ_wpre

   Plasmid

   #207522

   Purpose

   Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

   Depositor

   Bradley Hyman

   Article

   Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

   Insert

   TauFRET_P301L_3xKQ
   

   Use

   Lentiviral

   Tags

   NeonGreen and mTurquoise2

   Expression

   Mutation

   P301L; K311Q; K317Q; K321Q

   Promoter

   EF1a

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-CDH5-mRuby3-IRES-puro

    Plasmid

    #85144

    Purpose

    lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-PDK3

    Plasmid

    #23556

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDK3 (PDK3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pENTR-L4R1-hEF1a

    Plasmid

    #139523

    Purpose

    Provides constitutive promoter cassette between attL4-attR1 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    hEF1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

    Plasmid

    #124362

    Purpose

    Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-aptNFkB#5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

    Plasmid

    #85428

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-12
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AcGFP-MSH3

    Plasmid

    #215122

    Purpose

    Expresses AcGFP-MSH3 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MSH3 (MSH3 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMC-6-GFP-P2A-NptII-9

    Plasmid

    #197741

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    EGFP-P2A-NptII bicistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. lvl0 mKate2 CDS

    Plasmid

    #153394

    Purpose

    Far red fluorescent protein with SV40 NLS, codon optimised for Arabidopsis thaliana

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    mKate2 CDS
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1-FLAG-GFP-OSBP

    Plasmid

    #134659

    Purpose

    Flag and GFP-tagged OSBP

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    OSBP (OSBP Human)
    

    Use

    Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. HSC1-HS4-D4Z4c-GiP

    Plasmid

    #58541

    Purpose

    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pU6d:sgRNA#5

    Plasmid

    #64249

    Purpose

    expresses sgRNA under U6d promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHL-avitag3

    Plasmid

    #99847

    Purpose

    mammalian expression with secretive signal and avi tag for biotinylation

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Use

    Tags

    Avi

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF2018

    Plasmid

    #142311

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

    Plasmid

    #120563

    Purpose

    Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. Adeno EA

    Plasmid

    #64071

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Insert

    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Expression

    Mutation

    Promoter

    U6 and CBh

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

26. FKBP-mCherry

    Plasmid

    #108122

    Purpose

    Recruitable fluorophore

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL-p27UTR

    Plasmid

    #20881

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p27 3'UTR (Cdkn1b Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

28. CBX3 A6.5 gRNA

    Plasmid

    #90569

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CBX3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CBX3 (Guide Designation A6.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. N-Flag-IPO9_Lenti SFFV Puro WPRE

    Plasmid

    #217916

    Purpose

    Express IPO9 in mammalian cells

    Depositor

    Benjamin Cravatt

    Article

    Ogasawara et al Cell Chem Biol. 2024 Nov 7:S2451-9456(24

    Insert

    importin 9 (IPO9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAS_4xMx PylT_FLAG-Mx PylRS

    Plasmid

    #140011

    Purpose

    Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge