166,033 results

1. pDEST14-ARL8B

   Plasmid

   #67383

   Purpose

   Expression of native hArl8b in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8B (ARL8B Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. mCardinal-Keratin-17

   Plasmid

   #56164

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Keratin (KRT18 Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3-mRuby2

   Plasmid

   #40260

   Purpose

   RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Insert

   mRuby2
   

   Tags

   His6 and Xpress

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-SLC38A10_STOP

   Plasmid

   #161249

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC38A10 (SLC38A10 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. R777-E172 Hs.PIK3R1-nostop

   Plasmid

   #70456

   Purpose

   Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1(+)-NEMOb

   Plasmid

   #189934

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOb
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pcr4-12xMBS-PBS

   Plasmid

   #52984

   Purpose

   provide MBS-PBS alternating sequence

   Depositor

   Robert Singer

   Article

   Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

   Insert

   12xMBS-PBS
   

   Expression

   Bacterial

   Promoter

   none

   Available Since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 HA Arf6 ActQ67L

   Plasmid

   #10835

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 Q67L (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q67L. Defective in GTP hydrolysis. Constitutive…

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. SeeSaw Reporter (SSR2.0)

   Plasmid

   #50393

   Purpose

   SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.

   Depositor

   Pablo Huertas Sánchez

   Article

   Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

   Insert

   SSR2.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tandem p40p35

    Plasmid

    #108665

    Purpose

    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker

    Depositor

    Nevil Singh

    Insert

    interleukin-12 (Il12a, Il12b Mouse)
    

    Expression

    Mammalian

    Mutation

    IL-12 dimers have a Gly-Ser linker

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYMA3_SidK_GFP

    Plasmid

    #184276

    Purpose

    Legionella pneumophila SidK (1-278) - GFP

    Depositor

    John Rubinstein

    Article

    Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

    Insert

    SidK (1-278) (sidK Legionella pneumophila)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry

    Plasmid

    #122132

    Purpose

    Express a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    dCas9-10X GCN4-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dCas9 D10A & H840A

    Promoter

    TRE3G

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFP-Rab8A

    Plasmid

    #24898

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FLAG-MTOR-R2505P

    Plasmid

    #69015

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-R2505P (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Arginine 2505 to Proline

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HsEDC4_S

    Plasmid

    #147455

    Purpose

    Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsEDC4 (EDC4 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH2.10_Gag-MCP-P2A-eGFP

    Plasmid

    #205525

    Purpose

    Express HIV Gag-MCP-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Mucolipin1-pHcRed C1

    Plasmid

    #62959

    Purpose

    full length human Mucolipin-1 cloned into pHcRed1 C1

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Tags

    HcRed1

    Expression

    Mammalian

    Mutation

    Mucolipin-1 has 1 silent mutation compared to the…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. B2380_BTB-IDR

    Plasmid

    #215098

    Purpose

    Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR) in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    BTB-mCherry-FUS(IDR)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. nLuc-GLP-1R-His-FLAG

    Plasmid

    #124831

    Purpose

    Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

    Depositor

    Sam Gellman

    Article

    Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

    Insert

    GLP1R (GLP1R Human, Synthetic)
    

    Use

    Luciferase

    Tags

    FLAG, His6, and NanoLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. tetO-HAND2

    Plasmid

    #170690

    Purpose

    doxycycline-inducible overexpression of HAND2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. Mucolipin1 D471-472K-pHcRed C1

    Plasmid

    #62961

    Purpose

    full length human Mucolipin-1 D471/472K cloned into pHcRed1 C1

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    D471/472K (and two silent mutations T77 ACA inst…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3-TORCAR-NES

    Plasmid

    #175015

    Purpose

    Cytosol-targeted TORCAR.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    TORCAR-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP-C3-ZO-2

    Plasmid

    #27422

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pFUSE-rabbitFc1-IL2ss-VHH(G08)-His-Myc

    Plasmid

    #209857

    Purpose

    Plasmid coding for a nanobody (termed G08) against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

    Depositor

    Tim Brown

    Inserts

    IL2-ss
    VHH (G08 variant)
    Rabbit Fc Gamma 1
    

    Tags

    x3 MycTag and x6 HisTag

    Expression

    Mammalian

    Promoter

    hEF1-HTLV promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHR-UCOE-SFFV-EGFP

    Plasmid

    #188900

    Purpose

    Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAS_4xMma PylT sfGPF 150TAG

    Plasmid

    #154766

    Purpose

    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, U25C in PylT

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLEX-MCS UBC promoter

    Plasmid

    #128045

    Purpose

    Lentiviral vector with UBC promoter

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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