159,117 results

1. pCRZero

   Plasmid

   #120275

   Purpose

   Blunt-end cloning vector of PCR-products using ZERO-backgound system

   Depositor

   Ken Motohashi

   Article

   Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-TYR

   Plasmid

   #32781

   Depositor

   Ruth Halaban

   Article

   Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

   Insert

   Tyrosinase (TYR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-Neo-Bam APC

   Plasmid

   #16507

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ELONGIN BC (XX01TCEB1A-c001)

   Plasmid

   #110274

   Purpose

   Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C

   Depositor

   Nicola Burgess-Brown

   Insert

   Elongin BC (ELOC, ELOB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Elongin B is full length. Elongin C contains a.a…

   Promoter

   T7

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PE2-P2A-hMLH1dn

   Plasmid

   #174827

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PE2-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide-TagRFP-2A-BSD

   Plasmid

   #167930

   Purpose

   Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EML4-ALK variant 1

   Plasmid

   #183828

   Purpose

   Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-13 and ALK exon…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+-RecQL4

   Plasmid

   #211399

   Purpose

   mammalian expression of human RecQL4

   Depositor

   Helmut Pospiech

   Insert

   RecQL4 (RECQL4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-rtTA

   Plasmid

   #175274

   Purpose

   AAV vector mediating neuron-specific expression of rtTA

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Reverse tetracycline-controlled transactivator (rtTA)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRU45

    Plasmid

    #167667

    Purpose

    ENTRY vector for gRNA5 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ERK-KTR-mStrawberry reporter

    Plasmid

    #158686

    Purpose

    This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCARS1

    Plasmid

    #174868

    Purpose

    Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

    Depositor

    Zengyi Shao

    Article

    Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

    Insert

    ARS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 1066 pBabe puroL PTEN

    Plasmid

    #10785

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3/hArf3(WT)-HA

    Plasmid

    #79414

    Purpose

    Expresses C-terminally HA-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (ARF3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Fibronectin-human-EDA in pMAX

    Plasmid

    #120402

    Purpose

    enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

    Depositor

    Inaam Nakchbandi

    Article

    Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

    Insert

    EDA-fibronectin (FN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PAmCherry1-C1-P4Mx2

    Plasmid

    #202681

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    PAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG416GPD-ccdB

    Plasmid

    #14148

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE puro mouse IRS-1 myc

    Plasmid

    #11374

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-1 (Irs1 Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX307-FURIN-blast

    Plasmid

    #158461

    Purpose

    Constitutive lentiviral expression of human FURIN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    FURIN (FURIN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OPRK1-Tango

    Plasmid

    #66462

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    OPRK1 (OPRK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF2469

    Plasmid

    #142096

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IRF8 (IRF8 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC57-Curlcake1

    Plasmid

    #139340

    Purpose

    Expresses Curlcake1 (CC1) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake1 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC57-Curlcake2

    Plasmid

    #139341

    Purpose

    Expresses Curlcake2 (CC2) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake2 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC57-Curlcake4

    Plasmid

    #139343

    Purpose

    Expresses Curlcake4 (CC4) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake4 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMBP-hLIF

    Plasmid

    #179711

    Purpose

    Expresses H6-MBP-LIF

    Depositor

    Mark Arbing

    Insert

    Leukemia inhibitory factor (LIF Human)
    

    Use

    Tags

    His6-MBP-TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDF850

    Plasmid

    #228713

    Purpose

    Mammalian expression of EVOLVEpro PsaCas12f (epPsaCas12f)

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Science 0, eadr6006

    Insert

    epPsaCas12f
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    I178A/K333V/K454P

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4-STRAD alpha

    Plasmid

    #14889

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    STRAD alpha (STRADA Human)
    

    Use

    Tags

    6xHis/Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid

    #181744

    Purpose

    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY-HF1
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…

    Promoter

    T7

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-PRB

    Plasmid

    #89130

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold

    Plasmid

    #186981

    Purpose

    mammalian expression of Cas7-11 crRNA guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    mature DR guide
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET11a-hRanGAP1

    Plasmid

    #50941

    Purpose

    expression of full length untagged human RanGAP1 in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanGTPase activating protein 1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TRUPATH Triple Gagust

    Plasmid

    #196054

    Purpose

    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFN18A-HaloTag-Biotin

    Plasmid

    #206039

    Purpose

    HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

    Depositor

    Sergi Garcia-Manyes

    Article

    Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

    Insert

    Talin R3-IVVI (Tln1 Mouse)
    

    Use

    Tags

    10 x His-AviTag and Halo-Tag

    Expression

    Bacterial

    Mutation

    Threonine 809 to Isoleucine, Threonine 833 to Val…

    Promoter

    T7

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 3xFLAG-SENP7

    Plasmid

    #42886

    Depositor

    Edward Yeh

    Article

    Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

    Insert

    SENP7 (SENP7 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    T102A, R969C, L996S

    Promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCR4-24XPP7SL

    Plasmid

    #31864

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    24xPP7 Stem Loop Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAV106-pTDH3-mCherry-10xSumo-6xSIM

    Plasmid

    #116928

    Purpose

    Yeast integrative vector for expression of the SUMO-SIM complex

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    10xSUMO-6xSIM
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-minCMV-mCherry

    Plasmid

    #27970

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti Lifeact-iRFP670 BlastR

    Plasmid

    #84385

    Purpose

    Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge