161,273 results

1. pcDNA-FLAG-Hu-Trip6

   Plasmid

   #27255

   Depositor

   Thomas Gilmore

   Article

   Zhao et al Gene Expr. 1999 . 8(4):207-17.

   Insert

   Trip6 (TRIP6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pORANGE Dlg4-GFP KI

   Plasmid

   #131477

   Purpose

   Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Dlg4, Dlg4 Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAcBac2.tR4-OMeYRS/GFP*

   Plasmid

   #50831

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Tyr tRNA cassette
   EGFP*
   OMeYRS
   two copy Tyr tRNA cassette
   

   Use

   Baculoviral

   Tags

   His, Myc-6xHis, and WPRE

   Expression

   Insect

   Mutation

   Y39TAG and able to charge various unnatural amino…

   Promoter

   CAG, CMV, and U6 and H1

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET11phiC31poly(A)

   Plasmid

   #18942

   Depositor

   Michele Calos

   Article

   Groth et al Genetics. 2004 Apr . 166(4):1775-82.

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENN496_miniCRISPRcharm_dSaCas9

   Plasmid

   #220837

   Purpose

   mini CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   mini CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQE30-His-ThrRS

   Plasmid

   #124119

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Threonyl-tRNA ligase (thrS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. P1-EYFP-pA (Construct 5)

   Plasmid

   #55197

   Purpose

   Synthetic promoter responsive to gRNA1

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   EYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Ade MLP

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSET YBX-1

   Plasmid

   #13035

   Depositor

   Peter Newburger

   Article

   Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

   Insert

   YBX1 (YBX1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJYS1Ptac

    Plasmid

    #85545

    Purpose

    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac and unknown

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ADAMTS1_pCSdest

    Plasmid

    #53807

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    ADAMTS1 (ADAMTS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pLSSmKate1-C1

    Plasmid

    #31868

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSS-mKate1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    CMV

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NPY-td-Orange2

    Plasmid

    #83497

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Use

    Tags

    tandem mOrange2

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPRi(v1)-Blast

    Plasmid

    #170067

    Purpose

    Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHygR2r

    Plasmid

    #200072

    Purpose

    hygR selection nls-mScarlet marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHygG2r

    Plasmid

    #200069

    Purpose

    hygR selection nls-mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-endo-EKAR4

    Plasmid

    #205513

    Purpose

    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-EKAR4
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AbVec2.0-IGHG3

    Plasmid

    #99577

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype

    Depositor

    Hedda Wardemann

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-sfGFP

    Plasmid

    #125747

    Purpose

    sfGFP without iCreERT2 fusion

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMVHA SUZ12

    Plasmid

    #24232

    Purpose

    Mammalian expression of human SUZ12 with HA tag

    Depositor

    Kristian Helin

    Article

    Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDMyc-TNPO1

    Plasmid

    #86081

    Purpose

    A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP-Rab21-CCSS

    Plasmid

    #83424

    Purpose

    Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

    Promoter

    CMV

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTR-346 pUC19-tNGFR-P2A-IL2RA N

    Plasmid

    #186065

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RA (IL2RA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. myc-mTOR

    Plasmid

    #1861

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLPT107

    Plasmid

    #85525

    Purpose

    Repressilator with triple reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    mKate2
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS306-PHIS3-GFP-μNS

    Plasmid

    #116935

    Purpose

    Yeast integrative vector for the expression of μNS

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    μNS
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSBbi-GB

    Plasmid

    #60520

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMEXNH3

    Plasmid

    #110079

    Purpose

    Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His10 Tag - 3C cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-TRIM24

    Plasmid

    #28138

    Depositor

    Michelle Barton

    Article

    Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. moxCerulean3

    Plasmid

    #68066

    Purpose

    C-terminal fusion to moxCerulean3

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLOVE-miR302/367

    Plasmid

    #31310

    Depositor

    Ed Morrisey

    Article

    Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

    Insert

    mmu-miR302/367
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

33. SP-Cas9

    Plasmid

    #62731

    Purpose

    Expresses SP-Cas9 protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    SP-CAS9
    

    Use

    CRISPR

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET21a-His-IleRS

    Plasmid

    #124112

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Isoleucyl-tRNA ligase (ileS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pABY-T1

    Plasmid

    #201764

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHes7-Achilles-Hes7

    Plasmid

    #153528

    Purpose

    Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.

    Depositor

    Ryoichiro Kageyama

    Article

    Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:

    Insert

    Hes7 (Hes7 Mouse)
    

    Use

    Tags

    Achilles

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Hes7 promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

    Plasmid

    #112006

    Purpose

    For co-expression of GCaMP6s with mKate2

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    GCaMP6s-P2A-mKate2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHAGE2-TetOminiCMV-SKM

    Plasmid

    #136551

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3/hArf3(WT)-mCherry

    Plasmid

    #79420

    Purpose

    Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf3 (ARF3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-S5E2-ChR2-mCherry

    Plasmid

    #135634

    Purpose

    AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    ChR2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge