We narrowed to 24,865 results for: nes

Showing: 11401 - 11440 of 24865 results

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pH6HTC_FSCN1-Halo

Plasmid

#175343

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

FSCN1 (FSCN1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4

Plasmid

#220451

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Expression

Insect

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4DBD

Plasmid

#220452

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4DBD

Plasmid

#220454

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-p65AD

Plasmid

#220456

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4

Plasmid

#220449

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-LexAGAD

Plasmid

#220460

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Expression

Insect

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-Gal4DBD

Plasmid

#220453

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Expression

Insect

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-LexAGAD

Plasmid

#220459

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Expression

Insect

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-p65AD

Plasmid

#220455

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Expression

Insect

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-Gal4

Plasmid

#220450

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Expression

Insect

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-LexAGAD

Plasmid

#220458

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Expression

Insect

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-p65AD

Plasmid

#220457

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Expression

Insect

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-bsr

Plasmid

#165878

Purpose

Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd3-1D4

Plasmid

#42265

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
M-SPn-VP64

Plasmid

#48674

Purpose

Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pALPS tat-P2A-rev

Plasmid

#101331

Purpose

Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 tat and rev

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-mBmi1

Plasmid

#114317

Purpose

TET-inducible expression of the mBmi-1 protein

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

mBmi1 (Bmi1 Mouse)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaCC2

Plasmid

#124957

Purpose

Mammalian expression of Human TRIM28 with deleted CC domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of CC domains

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Alpha1

Plasmid

#165862

Purpose

Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSG424 Smad2 (Gal4-Smad2)

Plasmid

#14932

Depositor

Joan Massague

Article

Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

Insert

Smad2 (SMAD2 Human)

Tags

Gal4

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 beta-catenin Y654E

Plasmid

#16073

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

Y654E, *(see below)

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP RT-D185K/D186L

Plasmid

#101332

Purpose

pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 envFS

Use

Lentiviral

Mutation

reverse transcriptase D185K/D186L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR human DGCR8-2

Plasmid

#14770

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pLQ-KO-tgt50

Plasmid

#126578

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50

Use

CRISPR

Expression

Bacterial

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFLAG-4EHP

Plasmid

#17342

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
pMOD_A0108

Plasmid

#91001

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
OPN(-213)-luc

Plasmid

#31107

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

osteopontin promoter (SPP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -213 to +87

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Drosha1

Plasmid

#14780

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-VEGFR2-570

Plasmid

#21309

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-nDsRedFL-miR9/9*

Plasmid

#177805

Purpose

The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

U6

Available Since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-PT2A-GFP-iRFP670-Tdtomato

Plasmid

#111813

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
iRFP670
Tdtomato

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mFzd7-1D4

Plasmid

#42269

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 7 (Fzd7 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
HA-AMOT p130 delta linker

Plasmid

#32825

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D and amino acids 752-1076 deleted

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S94/F95A

Plasmid

#33057

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S94A, F95A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRU323

Plasmid

#167690

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-PT2A-iRFP670-GFP-Tdtomato

Plasmid

#111816

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
GFP
Tdtomato

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 24,865 results for: nes