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SH4-FRB-EBFP2

Plasmid

#222418

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

SH4

Tags

FRB-HA-EBFP2

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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8xGTIIC-luciferase

Plasmid

#34615

Purpose

YAP/TAZ-responsive synthetic promoter driving luciferase expression

Depositor

Stefano Piccolo

Article

Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

Insert

synthetic TEAD luciferase reporter

Use

Luciferase

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-EGFP

Plasmid

#52343

Purpose

AAVS1 donor vector for inducible gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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4 gRNA concatemer

Plasmid

#84881

Purpose

Empty concatmer vector in which 4 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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3X ERE TATA luc

Plasmid

#11354

Purpose

Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ERE

Use

Luciferase

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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Lact-C2-GFP

Plasmid

#22852

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 Domain (MFGE8 Bovine)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Martine Roussel, Peter ten Dijke

Article

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Expression

Mammalian

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f

Plasmid

#162373

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

CMV

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTNS2

Plasmid

#64968

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

tnsABCD

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTXB1-Tn5

Plasmid

#60240

Purpose

IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

Depositor

Rickard Sandberg

Article

Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

Insert

tnpA from pBam1

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7 promoter

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.27_ARE/NRF2-SPE

Plasmid

#177775

Purpose

Luciferase reporter for ARE/NRF2

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

NRF2 (NFE2L2 Human)

Use

Luciferase

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-5SA/S94A

Plasmid

#33103

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S61A, S94A, S109A, S127A, S164A, S381A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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eeBxb1_IVT

Plasmid

#222342

Purpose

Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Template for in vitro transcription

Promoter

T7 (inactivated)

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJPC596 FoMV-AmCyan

Plasmid

#234812

Purpose

To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

AmCyan

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET-MBP-mSA2

Plasmid

#52319

Purpose

Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

Depositor

Sheldon Park

Article

Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

Insert

monomeric streptavidin 2

Tags

FLAG, His, MBP, and TEV

Expression

Bacterial

Mutation

S25H mutation compared to streptavidin

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Article

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Expression

Bacterial

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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TLCV2

Plasmid

#87360

Purpose

LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Insert

Cas9-2A-eGFP

Use

CRISPR and Lentiviral; Doxycycline inducible; egf…

Tags

Cas9-T2A-eGFP

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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SF-SON IRES Tomato

Plasmid

#214403

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

Depositor

Tanja Kuhlmann

Article

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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Alpha-Synuclein-His

Plasmid

#186802

Purpose

Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

Alpha-Synuclein-His (SNCA Human)

Tags

His6

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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