166,806 results

1. PGKp-GFP-YAP (S94A)

   Plasmid

   #174173

   Purpose

   Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Serine 94 mutated to Alanine

   Promoter

   PGK

   Available Since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pFUW-tetO-GATA2

   Plasmid

   #125028

   Purpose

   doxycycline-inducible expression of human GATA2 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

   Insert

   GATA binding protein 2 (GATA2 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p2attNG-H11-long

   Plasmid

   #51545

   Purpose

   p2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   GFP4m
   

   Use

   Human targeting

   Expression

   Mammalian

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF0922

   Plasmid

   #142207

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR2F1 (NR2F1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SUPV3L1-HA WT

   Plasmid

   #232348

   Purpose

   Expresses human SUPV3L1/SUV3 in mammalian cells with HA tag

   Depositor

   Samantha Lewis

   Article

   Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

   Insert

   SUPV3L1 (SUPV3L1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-N2cN

   Plasmid

   #100801

   Purpose

   express rabies virus CVS N2c strain nucleoprotein gene (N2cN)

   Depositor

   Ian Wickersham

   Insert

   rabies virus CVS N2c strain nucleoprotein gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF5/FRT/DEST-3xHA/mGli2

   Plasmid

   #51250

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCAG/ATP10A-HA

   Plasmid

   #209215

   Purpose

   Mammalian expression of ATP10A

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP10A (ATP10A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

   Plasmid

   #51248

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907,980,1006 mutated to A…

   Promoter

   EF1a

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pTCF6 HLA-A*24:02

    Plasmid

    #180445

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*24:02
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-PACT

    Plasmid

    #50352

    Purpose

    bacterial expression of PACT

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    PACT (PRKRA Human)
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJL1-mCherry

    Plasmid

    #102629

    Purpose

    In vitro expression of mCherry from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX304-VHL-IRES-Tdtomato

    Plasmid

    #187643

    Purpose

    VHL Expression vector with Tdtomato

    Depositor

    William Kaelin

    Article

    Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

    Insert

    VHL (VHL Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS and P2A

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAN-PTet-dCas9

    Plasmid

    #62244

    Purpose

    controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    catalytically inactive, D10A, H840A

    Promoter

    Ptet

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA5/mCherry_pBirA-HA_FKBP8

    Plasmid

    #154457

    Purpose

    expresses mCherry_pBirA-HA_FKBP8/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    mCherry_pBirA-HA_FKBP8 (FKBP8 Human)
    

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.18 NR0B1-Luc

    Plasmid

    #100983

    Purpose

    NR0B1 luciferase reporter - firefly luciferase driven by NR0B1 promoter

    Depositor

    Lee Helman

    Article

    Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

    Insert

    NR0B1 (NR0B1 Human)
    

    Expression

    Mammalian

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICH-roGFP2-synORP1

    Plasmid

    #191680

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    rredox(roGFP2-synORP1
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDisplay-GRAB_UCN1.0

    Plasmid

    #208667

    Purpose

    Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV UBC APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #214983

    Purpose

    Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    APEX2 (APX2 Glycine max (soybean))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Promoter

    UBC

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge