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pDONR223_EGFR_p.774_775insHV

Plasmid

#82852

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

HV insertion between AA 774 and 775

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Basigin

Plasmid

#221415

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

Basigin (BSG Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-N1

Plasmid

#31925

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pCXLE_hePOU_shp53

Plasmid

#206397

Purpose

Integration-free (episomal) expression of human ePOU and shRNA against p53.

Depositor

Ralf Jauch

Inserts

ePOU
shRNA against p53 (TP53 Human)

Expression

Mammalian

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CSAC-Pers

Plasmid

#168281

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmEMBer

Plasmid

#174441

Purpose

Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEMBer

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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JAB2165

Plasmid

#190348

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::QacR-VP16-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::QacR-VP16-19St

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2164

Plasmid

#190347

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::LmrA-VP16-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::LmrA-VP16-19St

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG551-MAP4-greenFAST

Plasmid

#160366

Purpose

Expresses MAP4-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

MAP4-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMC-1-PvUbi2-6

Plasmid

#197706

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi2 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-1

Plasmid

#210133

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAX-2

Plasmid

#210130

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAK-1

Plasmid

#210131

Purpose

knock out BAK in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Bcl-2 homologous antagonist/killer (BAK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.ELREA746del_T790M

Plasmid

#82917

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

T790M; p.ELREA746del

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MIGS2.2 (FF573)

Plasmid

#35248

Purpose

Expression of miR173 precursor under UBQ10 promoter; includes miR173 target site after 35S promoter (Kanamycin selection marker)

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Expression

Plant

Promoter

CaMV 35S

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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AiP12498 - pAAV-AiE0395m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2498)

Plasmid

#208147

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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CSAC-Bmal1

Plasmid

#168296

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgBmal1-1-hU6-sgBmal1-3

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

Plasmid

#172609

Purpose

Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

2xFLAG-mAID-AMBRA1 (AMBRA1 Human, Synthetic)

Use

CRISPR; Human targeting

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-R4R3-MC1DTA

Plasmid

#139521

Purpose

Provides negative selection cassette on the vector

Depositor

Minoru Ko

Type

Empty backbone

Use

Mouse Targeting

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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