4,272 results

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pET28c-F30-2xdBroccoli

Plasmid

#66843

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Article

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCRY2PHR-mCherryN1

Plasmid

#26866

Purpose

Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2PHR (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-NbALFA

Plasmid

#159986

Purpose

For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mEGFP-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mEGFP and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ADAM10-HA

Plasmid

#65106

Purpose

Expression of HA tagged ADAM10 in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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5HRE/GFP

Plasmid

#46926

Purpose

hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

Depositor

Martin Brown, Thomas Foster

Article

Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

Insert

5X HRE of VEGF (VEGFA Human)

Tags

d2EGFP

Expression

Mammalian

Mutation

five copies of a 35-bp fragment from the hypoxia-…

Promoter

EF-1α promoter

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

Plasmid

#138150

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTP1172

Plasmid

#104161

Purpose

Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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mApple-N1

Plasmid

#54567

Purpose

Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mApple

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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Hsp90 HA

Plasmid

#22487

Depositor

William Sessa

Article

Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

Insert

Hsp90 HA (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Article

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV

Plasmid

#69929

Purpose

bacterial expression of MBP-TEV n-terminal fusions

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

MBP and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-p300-P2A-PuroR

Plasmid

#83889

Purpose

Lentiviral Sp dCas9-p300-P2A-PuroR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
pac from Streptomyces alboniger

Use

CRISPR and Lentiviral

Tags

Flag and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EFS

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pXR001: EF1a-CasRx-2A-EGFP

Plasmid

#109049

Purpose

CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

CasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLX302_FOXA1-V5

Plasmid

#70090

Purpose

Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pDESTtol2pACrymCherry

Plasmid

#64023

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

mCherry

Use

Gateway

Promoter

alpha-Acrystallin promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2

Plasmid

#40978

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pDisplay-mSA-EGFP-TM

Plasmid

#39863

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin, EGFP, TM helix

Tags

EGFP, FLAG, and HA

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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