We narrowed to 29,779 results for: REP
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Plasmid#220048PurposeReporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.DepositorInsertflag-HA-stop-GFP
UseTagsExpressionMammalianMutationPromoterAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN013
Plasmid#220050PurposeReporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.DepositorInsertGFP-stop-fLuc
UseTagsExpressionMammalianMutationPromoterAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
β2AR-PPI-CALPon1.0 (APEX)
Plasmid#213406PurposePeroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)DepositorInsertβ2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ca-LiPPI-CLAPon1.0 (TEVp)
Plasmid#213409PurposeLight-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)DepositorInsertCaM-V5-TEVp
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACBE-FSR (pXK-1648)
Plasmid#208821PurposeUniversal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.DepositorTypeEmpty backboneUseTagsEGFPExpressionMammalianMutationPromoterCMV, U6Available SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT353
Plasmid#204506PurposeIn vitro transcription of RNA probes for in situ hybridization of CeRep55 lncRNAs in nematode (after linearization with NotI and BglI)DepositorInsertCeRep55 tandem repeats from Y73B3A (C. elegans)
UseOtherTagsExpressionMutationPromoterdouble-T7Available SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-GvpB
Plasmid#173798PurposeMammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)DepositorInsertGvpB
UseTagsmCherry (K187N -please see depositor comments)ExpressionMammalianMutationPromoterTRE3G promoterAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0597
Plasmid#196166PurposeMoClo Level 1 luciferase reporter: MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0602
Plasmid#196168PurposeMoClo Level 1 luciferase reporter: MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0601
Plasmid#196167PurposeMoClo Level 1 luciferase reporter: MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0604
Plasmid#196169PurposeMoClo Level 1 luciferase reporter: MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0607
Plasmid#196171PurposeMoClo Level 1 luciferase reporter: MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0608
Plasmid#196172PurposeMoClo Level 1 luciferase reporter: MinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0595
Plasmid#196170PurposeMoClo Level 1 luciferase reporter: MinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP728_L2_pV01_0I_FRT-TB-rLUC_TCTP-fLUC
Plasmid#192371PurposeTo test for the repressive potential of the Transcription Block (TB) with the FRT sites around it.DepositorInsertAct2::FRT-TB-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-TetO-GFP_ColE1
Plasmid#173193PurposeExpresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.DepositorInsertT7-TetO
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term SMT3 NikJC199U
Plasmid#174210PurposeExpression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term SMT3 NikJC199U
Plasmid#174362PurposeExpression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP NikJC199U
Plasmid#174364PurposeExpression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, and TEVExpressionBacterialMutationPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG-altTAG
Plasmid#158146PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG
Plasmid#158145PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BYG
Plasmid#158144PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertBFP-GFP fragment (wild-type)
UseTagsExpressionYeastMutationPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6
Plasmid#158142PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertFAPB2.3.6
UseTagsExpressionYeastMutationPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6L1TAG
Plasmid#158143PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertFAPB2.3.6L1TAG
UseTagsExpressionYeastMutationPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-1
Plasmid#136385PurposeExpresses DANCER-1 dynamic proteinDepositorInsertDANCER-1
UseTags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (GB1 WT - A34F/V39L)
Plasmid#136610PurposeExpresses GB1 WT double mutant (A34F/V39L)DepositorInsertGB1 WT double mutant (A34F/V39L)
UseTags6x-HistagExpressionBacterialMutationMutation from GB1 WT: A34F - V39LPromoterT7 promoterAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-E2A-cyOFP1
Plasmid#141084Purposeprecursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-E2A-cyOFP1
UseLentiviralTagsExpressionMutationPromoterCMVAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3
Plasmid#133343Purposefor constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_gcrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA
Plasmid#133345Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_blaA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
LGhG-pHG165a
Plasmid#90048PurposeConstitutive expression of a chimeric LacI:GalR transcription repressorDepositorInsertLGhG
UseTagsExpressionBacterialMutationPromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only