We narrowed to 19,819 results for: Cre
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Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121804PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121808PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter
Plasmid#121818PurposepMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestionDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF8_P2A_Hygro_Barcode
Plasmid#120494PurposeBarcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH2charge
Plasmid#105858PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
ExpressionMammalianMutationmutated heavy chain of mouse IgG3, muatations: Hi…PromoterhEF1-HTLV promAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-Notch1 3'UTR-mir200c-mut
Plasmid#84596PurposeRenilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding siteDepositorInsertNOTCH1 3'UTR (NOTCH1 Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on NOTCH1 3…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-LFNG 3'UTR-mir200c-mut
Plasmid#84594PurposeRenilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding siteDepositorInsertLFNG 3'UTR (LFNG Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on LFNG 3'…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4c-AP-His
Plasmid#71959PurposeExpresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S279A
Plasmid#60705Purposeretroviral expression of rat Cx43 S279A mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-S279/282A
Plasmid#60712Purposemammalian expression of rat Cx43 S279/282A mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-S279/282D
Plasmid#60714Purposemammalian expression of rat Cx43 S279/282D mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pU6-(BbsI)_CBh-Cas9-T2A-BFP
Plasmid#64323PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-EBFP2ExpressionMammalianPromoterCBh and U6Available SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_RUNX1_P2A_Hygro_Barcode
Plasmid#120475PurposeBarcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY093-EFS-BCMA-Blast-WPRE
Plasmid#192194PurposeLenti-BCMA-OE-BlastDepositorAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
Plasmid#64324PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh and U6Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-BRD4
Plasmid#65378PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Blast
Plasmid#199622PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLI1_pLENTI-CAG-IRES-GFP
Plasmid#176992PurposeMammalian lentiviral expression vector encoding GLI1DepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-ATF4 mScarlet NLS
Plasmid#115969PurposeER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-IDepositorInsertactivating transcription factor 4 (ATF4 Synthetic, Human)
UseLentiviralTagsHA tag, c-myc NLS, and mScarlet-IExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-D614 (C-flag)
Plasmid#156420PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-2xFYVE
Plasmid#140050PurposeExpresses mCherry-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
TagsmCherryExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailable SinceMay 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV
Plasmid#216156PurposeExpresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with C-terminal FLAG tag
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-UltraCas12a-6xNLS
Plasmid#209023PurposeLentiviral vector expressing human codon-optimized UltraCas12a with 6xNLSDepositorInsertUltraCas12a-6xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationM537R/F870LPromoterEF-1aAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_HNF1A_P2A_Hygro_Barcode
Plasmid#120448PurposeBarcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
BACE2_pcDNA6.2/EmGFP-Bsd
Plasmid#176942PurposeMammalian expression vector encoding BACE2 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Sec61b-C1
Plasmid#90992PurposeExpresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorInsertmEmerald-Sec61b (SEC61B Synthetic, Human)
TagsmEmerald (green fluorescence)ExpressionMammalianPromoterCMVAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CXCR5-DuET
Plasmid#213222PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET01-TAF5-ex8-Minigene-SRSF1 motif WT
Plasmid#218972PurposeVector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)DepositorInsertTAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
ExpressionMammalianPromoterRSV LTRAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
InsRA-eGFP
Plasmid#79795PurposeInsulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.DepositorAvailable SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc-LATS2
Plasmid#66852PurposeExpress Myc-tagged LATS2 in mammalian cellsDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-ASCL1-T2A-PuroR
Plasmid#162345PurposeLentiviral expression of ASCL1 under the control of the TetON promoterDepositorAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAB10
Plasmid#130883PurposeExpresses human RAB10 with N-terminal GFP tagDepositorAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only