We narrowed to 9,184 results for: Pol;

1. pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

   Plasmid

   #177887

   Purpose

   Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Tags

   10xHis-TEV-4xFRB-SNAP tag

   Expression

   Insect

   Mutation

   G2A

   Promoter

   Polyhedrin

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Endo-INPP5E

   Plasmid

   #236005

   Purpose

   Endosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

   Depositor

   Jin Zhang

   Article

   Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

   Insert

   Endo-INPP5E (INPP5E Synthetic, Human)
   

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-HA-Ubiquitin-K63

   Plasmid

   #17606

   Purpose

   Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K63 only, other lysines mutated to arginines. Enh…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-HA-Ubiquitin-K48

   Plasmid

   #17605

   Purpose

   Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48 only, other lysines mutated to arginines. Enh…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1 flag-cFLIP

   Plasmid

   #211529

   Purpose

   Expresses flag-tagged cFLIP (CFLAR)

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   cFLIP (CFLAR Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

   Plasmid

   #170186

   Purpose

   This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

   Depositor

   Bradley Bernstein

   Article

   Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

   Insert

   CreERT2-T2A-eBFP2
   

   Use

   Lentiviral

   Promoter

   EF1 alpha

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV4-ApoE3

   Plasmid

   #87086

   Purpose

   Expresses ApoE3 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E3 (APOE Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIV-EGFP.Flag-EZH2

   Plasmid

   #220243

   Purpose

   Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc-C Med12His

   Plasmid

   #49240

   Purpose

   expresses human Med12 with His tag in insect cells

   Depositor

   Dylan Taatjes

   Article

   Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

   Insert

   Med12 (MED12 Human)
   

   Tags

   His

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV4-ApoE4

    Plasmid

    #87087

    Purpose

    Expresses ApoE4 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E4 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-MYO7A-TAIL

    Plasmid

    #89585

    Purpose

    Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO7A (Myo7a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation encoding amino acids 870-2166 of MYO7A…

    Promoter

    CMV

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. HAP40 1-371

    Plasmid

    #124060

    Purpose

    Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HAP40 (F8A1 Human)
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Promoter

    polyhedrin

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-hPPP3CA-FLAG

    Plasmid

    #179134

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MOM603

    Plasmid

    #133242

    Purpose

    expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

    Depositor

    Shinsuke Niwa

    Article

    Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

    Insert

    kif1a (KIF1A Human)
    

    Tags

    StrepII tag and mScarlet

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB1.HMBP.PrS.MTF2

    Plasmid

    #125169

    Purpose

    Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    MTF2 (MTF2 Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. GIPR-DuET

    Plasmid

    #213252

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GIPR (GIPR Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZsGreen1-cMYC/pLVX-Puromycin

    Plasmid

    #180278

    Purpose

    Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    ZsGreen1-cMYC fusion protein MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 TAP DHX36

    Plasmid

    #53547

    Purpose

    mammalian expression of DHX36

    Depositor

    Kathleen Collins

    Article

    Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    TAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMP71-hCD155

    Plasmid

    #118630

    Purpose

    human CD155 coding sequence subcloned into pMP71 retroviral plasmid

    Depositor

    Sébastien Walchli

    Article

    Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

    Insert

    CD155 (PVR Human)
    

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid

    #224545

    Purpose

    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    WPRE-SV40-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTEF:ATP

    Plasmid

    #92179

    Purpose

    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    

    Use

    Integration and expression into yeast.

    Tags

    Yeast codon optimized mseCFP (donor) and cp173-mV…

    Expression

    Yeast

    Promoter

    TEF1p

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA6-N-3XFLAG-Dvl2

    Plasmid

    #123587

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    dishevelled segment polarity protein 2 (Dvl2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid

    #180484

    Purpose

    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

    Depositor

    Julie Welburn

    Article

    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

    Insert

    CENP-E (CENPE Synthetic, human, Human)
    

    Tags

    mNeon-6xHis

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. KAT14-pDEST10

    Plasmid

    #118382

    Purpose

    HIS-tagged insect cell expression vector with KAT14.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    KAT14 (KAT14 Human)
    

    Tags

    HIS

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pFB1.HMBP.PrS.PHF1

    Plasmid

    #125167

    Purpose

    Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF1 (PHF1 Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. AAV-Syn-NS1

    Plasmid

    #175276

    Purpose

    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    NLS-dTomato (P2A cleavage)

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. Ratiometric FPX sensor for ERK kinase activity

    Plasmid

    #60974

    Purpose

    For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A-WW domain-ERK substrate-ddFP B
    

    Tags

    ERK substrate is before ddFP copy B, WW domain is…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

29. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208041

    Purpose

    Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    TurboID
    

    Use

    Lentiviral

    Promoter

    EFS

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. AAV-TRE-NS1-dTomato

    Plasmid

    #175278

    Purpose

    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    NS1; dTomato (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    dTomato (from bidirectional promoter)

    Promoter

    bidirectional TRE promoter

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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