20,205 results

Showing: 11721 - 11760 of 20205 results

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pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-hHelios-IRES-GFP

Plasmid

#74047

Purpose

retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Helios (IKZF2) (IKZF2 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

encodes shorter version of human Helios

Promoter

pMSCV-LTRs

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-wtSrc

Plasmid

#140294

Purpose

Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Tags

2X HA

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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PTEN shRNA

Plasmid

#86645

Purpose

expression of shRNA targeting PTEN

Depositor

Jaime Modiano

Article

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (PTEN Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pETMutS

Plasmid

#13245

Depositor

Peggy Hsieh

Article

Biswas et al J Biol Chem 1996 Mar 1;271(9):5040-8.

Insert

MutS

Expression

Bacterial

Mutation

None

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Article

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Insert

KSRP (KHSRP Human)

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-tag

Plasmid

#55180

Purpose

FLAG-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

FLAG

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V2

Plasmid

#73638

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pWPXL-c-Myc

Plasmid

#36980

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

c-Myc (Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-A1Up

Plasmid

#16170

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Ubiquilin 4 (UBQLN4 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
MSCV-MYC-t2A-BCL2

Plasmid

#135306

Purpose

Overexpression of multiple human genes (BCL2 and MYC)

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

MYC (MYC Human)
BCL2 (BCL2 Human)

Use

Retroviral

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Ran(Q69L)

Plasmid

#42048

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Ran (RAN Human)

Tags

His

Expression

Bacterial

Mutation

Q69L

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR

Plasmid

#171382

Purpose

Expression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized dCas13X.1-REPAIRv2

Tags

HA

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1

Plasmid

#155346

Purpose

Express full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (YTHDF1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAd5-B1

Plasmid

#122551

Purpose

Block 1 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Promoter

None

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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CyPET-Arf6

Plasmid

#18840

Depositor

Martin Schwartz

Article

Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

Insert

CyPET-Arf6 (ARF6 Human)

Expression

Mammalian

Mutation

The sequence for the fluorescent protein CyPET wa…

Available Since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only
-1748 human cyclin D1 promoter pGL3Basic

Plasmid

#32726

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Reporter gene

Promoter

-1748CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Hs.KRAS4b G12V

Plasmid

#83132

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12V

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Casp8 C360A

Plasmid

#11818

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 catalytic mutant (CASP8 Human)

Tags

HA

Expression

Mammalian

Mutation

C360A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
PX851

Plasmid

#62883

Purpose

N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEx-4T-1-YTHDF1

Plasmid

#70087

Purpose

Encode YTHDF1 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAGI1

Plasmid

#23523

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAGI1 (MAGI1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTKEI-Tre-C04

Plasmid

#79754

Purpose

Single fluorescent protein biosensor for trehalose

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Tre-C04

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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mEmerald-N-Wasp-C-18

Plasmid

#54199

Purpose

Localization: Cytoskeleton-Signalling , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

N-Wasp

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_078

Plasmid

#158582

Purpose

Lentiviral expression of BE4max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PRKAB1

Plasmid

#23360

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAB1 (PRKAB1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
12xMoonTag-12xSunTag-kif18b-24xPP7

Plasmid

#128606

Purpose

Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

12xMoonTag-12xSunTag
kinesin family member 18b (KIF18B Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dLight1.1

Plasmid

#111053

Purpose

Expresses dLight1.1 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.1

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-dLight1.3b

Plasmid

#125560

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1, AAV5, and AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ150 - pDESTttTi5605[R4-R3]

Plasmid

#19329

Depositor

Erik Jorgensen

Article

Christian et al

Insert

pDESTttTi5605[R4-R3]

Expression

Worm

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet_NADP-Snifit-mito

Plasmid

#113919

Purpose

Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNANOG-Luc

Plasmid

#25900

Depositor

Ren-he Xu

Article

Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

Insert

NANOG promoter (NANOG Human)

Tags

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
hSynapsin-PE2-Cterminal-lenti

Plasmid

#135956

Purpose

Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) C-terminal

Use

Lentiviral

Expression

Mammalian

Mutation

MMLV RT(D200N, T306K, W313F, T330P, L603W)

Promoter

hSynapsin

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT/TO/HIS/FLAG/HA-HNRNPD

Plasmid

#38066

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

HNRNPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mMaroon1

Plasmid

#83840

Purpose

Expresses mMaroon1

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mMaroon1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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20,205 results