161,799 results

1. GESTALT_pX330-v1

   Plasmid

   #103061

   Purpose

   plasmid px330 with guide targeting GESTALT v1 through V5 constructs

   Depositor

   Jay Shendure

   Article

   McKenna et al Science. 2016 May 26. pii: aaf7907.

   Insert

   integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lamin A-mRFP

   Plasmid

   #124268

   Purpose

   Wild-type Lamin A, C-terminally tagged with mRFP

   Depositor

   Eric Schirmer

   Insert

   LMNA (LMNA Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL2680

   Plasmid

   #85581

   Purpose

   Replicating base vector for constructing cpf1/CRISPR editing plasmids

   Depositor

   Himadri Pakrasi

   Article

   Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ204-loxP-100RPU.2-coEPO-lox2272

   Plasmid

   #154828

   Purpose

   Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   coEPO
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized (Cricetulus griseus)

   Promoter

   100RPU.2

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LifeAct-mGarnet

   Plasmid

   #104307

   Purpose

   codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   LifeAct-mGarnet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-TadA8e-Ns3h

   Plasmid

   #221227

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and Ns3h helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-Ns3h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IB-TMEM192-mKeima

   Plasmid

   #215359

   Purpose

   Probe to evaluate lysophagic activity

   Depositor

   Tamotsu Yoshimori

   Article

   Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

   Insert

   transmembrane protein 192 (TMEM192 Human)
   

   Use

   Retroviral

   Tags

   mKeima

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

   Plasmid

   #210016

   Purpose

   episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   CAG

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_3xFLAG-ATP6V0A3

   Plasmid

   #228866

   Purpose

   Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V0A3 (TCIRG1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. EYFP WC visual barcode

    Plasmid

    #158675

    Purpose

    tagging clonal populations with whole cell EYFP fluorescent protein

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    EYFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-ARAP3

    Plasmid

    #39484

    Depositor

    Sonja Vermeren

    Article

    Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

    Insert

    ARAP3 (ARAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-Wnt4-V5

    Plasmid

    #35929

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt4 (WNT4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Silent Mutation C (492) --> T

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-Rab21

    Plasmid

    #83421

    Purpose

    Mouse Rab21 (wild type)

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM47523

    Plasmid

    #153221

    Purpose

    Level 0 zCas9i vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    SpCas9 with introns and 2 NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROP_C9_Puro_AAVS1A

    Plasmid

    #183337

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    AAVS1A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tet-O-FUW-EGFP

    Plasmid

    #30130

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    Enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Frankenbody-FLAG-Halo

    Plasmid

    #175296

    Purpose

    Track mature and nascent FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with HaloTag
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pet-15b-ultraID

    Plasmid

    #172879

    Purpose

    expresses ultraID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209652

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    CAG

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p1478 HPV-6 E6

    Plasmid

    #10872

    Depositor

    Peter Howley

    Article

    Ronco et al Genes Dev. 1998 Jul 1. 12(13):2061-72.

    Insert

    HPV-6b E6 (E6 Human papillomavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

21. pBabe K-Ras 12V

    Plasmid

    #12544

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    K-Ras 12V (KRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    12V constituitively activated

    Promoter

    Available since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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22. His-GFP-Syntaxin1A

    Plasmid

    #170871

    Purpose

    Mammalian expression of His-GFP-Syntaxin1A.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Syntaxin 1A (Stx1a Mouse)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCherry-Centrin2-N-10

    Plasmid

    #55018

    Purpose

    Localization: Centrosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Centrin2 (CETN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PLK3 1-334-pEGFP

    Plasmid

    #23266

    Depositor

    Wei Dai

    Article

    Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

    Insert

    PLK3 (PLK3 Human)
    

    Use

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    aa 1-334

    Promoter

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pTRIP-CMV-mCherry-53BP1

    Plasmid

    #127658

    Purpose

    Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. lenti-CDDR

    Plasmid

    #149355

    Purpose

    CRISPR/Cas9-based dual-fluorescent DSB repair reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    CDDR Reporter
    Blasticidin resistance
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hPGK

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAV-U6+27-Tornado-biSiRA

    Plasmid

    #196345

    Purpose

    Overexpression of circular biSiRA

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    Tornado-biSiRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid

    #114085

    Purpose

    CAG promoter expression plasmid for human codon optimized LbBE1.1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLv-HSA-uDys/eGFP

    Plasmid

    #26810

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    dystrophin (DMD Human)
    

    Use

    Lentiviral; Lentiviral plasmid backbone from l. n…

    Tags

    eGFP

    Expression

    Mutation

    deleted R4-R23 deleted exons 71-78

    Promoter

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73037

    Purpose

    Express Nluc and copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge