159,557 results
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Plasmid#216109PurposeaTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT3630 signal peptideExpressionBacterialMutationPromoterP1TDP-A21Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG HRP-TM
Plasmid#44441DepositorArticleInsertHorseradish peroxidase fused to the transmembrane domain of PDGFR
UseTagsHA tag and myc tagExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
UseTagsExpressionBacterialMutationAraC: L133F E165L E169G C280LPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-NLS-I-PpoI
Plasmid#46963PurposeFor constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.DepositorInsertNLS-I-PpoI
UseTagsHAExpressionMammalianMutationPromoterCMV-TETAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5 c-Fyn
Plasmid#16032DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEMS1995
Plasmid#49125PurposeAAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.DepositorInsertssAAV-Ple94-iCre
UseAAVTagsExpressionMammalianMutationPromoterGPR88Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-NBRE3
Plasmid#208254PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X NBRE-LUC
UseTagsExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-HIS3MX6
Plasmid#227981PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1
Plasmid#27156PurposeFor mammalian expression of human Elk-1 with an HA tagDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-E988K
Plasmid#173945PurposeBacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)DepositorInsertPARP1-E988K (PARP1 Human)
UseTags6xHisExpressionBacterialMutationV762A, E988KPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
RAR-TRE-mStrawberry reporter
Plasmid#158683PurposeThis plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4-AARE-luc2P-Hygro
Plasmid#101787PurposeLuferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)DepositorInsert3xAARE
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC12-10XUAS-IVS-myr::GFP
Plasmid#26222DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Hph
Plasmid#188741PurposepUC19 plasmid (MCS intact) with Hygromycin selection markerDepositorInsertHygromycin
UseFilamentous fungal expressionTagsExpressionMutationPromotergpdAAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-pur-CAG-Bi-DREADD
Plasmid#159457PurposeAAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection geneDepositorInsertBi-DREADD
UseTagsExpressionMammalianMutationfusion protein of hM3Dq-mCherry and HA-KORD seper…PromoterCAGAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHSB04X
Plasmid#117258PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertCas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
eMagAF-EGFP-PM
Plasmid#162247PurposeBlue-light dependent heterodimerization systemDepositorInserteMagAF-EGFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-QF2
Plasmid#83307Purposecodes for QF2 (truncated QF) in a gateway middle entry backbone.DepositorInsertQF
UseTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
hMLKL-Venus
Plasmid#106078Purposeexpression in mammalian cellsDepositorInserthuman MLKL-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianMutationPromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-EGFP-alpha Tubulin
Plasmid#12298DepositorInsertEGFP-alpha Tubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
miR155 in pcDNA3.1
Plasmid#78126PurposeMammalian expression of miR155DepositorInsertmiR155 (MIR155 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5068 pGEX-6P-1 Brd4 full-length
Plasmid#14447DepositorInsertBrd4 (BRD4 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
yo-msGFP2
Plasmid#160471PurposeEncodes a yeast codon optimized version of msGFP2DepositorInsertyo-msGFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMT-flp
Plasmid#67274PurposeConjugally transferable Flp recombinase plasmid for unmarked mutagenesisDepositorInserttetA, flp, repA101, oriR101
UseFlp recombinase plasmid, temperature sensitiveTagsHis-SUMO (flp)ExpressionMutationPromotertetA promoter of plasmid pACYC184 originAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1
Plasmid#92120PurposeExpression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.DepositorInsertMS2-P65-HSF1 (HSF1 Synthetic, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme WT
Plasmid#18110DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKH116_keima-YIPF3
Plasmid#214970PurposeGolgiphagy flux reporter keima-YIPF3DepositorInsertkeima-YIPF3 (YIPF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-Lepr_FL-IRES-eGFP
Plasmid#196635PurposeDoxycycline inducible expression of full length mouse Lepr with eGFP markerDepositorInsertLepr (Lepr Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi
Plasmid#27320DepositorInsertzebrafish ubiquitin promoter (ubb Zebrafish)
UseMultisite gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBbS2c-RFP
Plasmid#35329DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTetAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEZYflag
Plasmid#18700PurposepFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cellsDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSEZ-1
Plasmid#51868PurposeExpression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegansDepositorInsertpsdhb-1::mtLS-cpYFP
UseTagsExpressionWormMutationPromotersdhb-1Available sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWXC077
Plasmid#222960PurposeC. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backboneDepositorInsertmScarlet3
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC2-Gephyrin P1
Plasmid#68820PurposemCherry tagged Geph for mammalian expressionDepositorInsertGephyrin (Gphn Rat)
UseTagsmCherryExpressionMammalianMutationsilent mutations on internal EcoRI site. Gephyrin…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-MCL1
Plasmid#202663PurposeTet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassetteDepositorInsertMCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-ASCL1-T2A-PuroR
Plasmid#162345PurposeLentiviral expression of ASCL1 under the control of the TetON promoterDepositorInsertASCL1 (ASCL1 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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PH-PLCd1-mNGx1
Plasmid#202751Purposeexpresses fluorescent protein-tagged lipid biosensorDepositorInsertPLCD1v2(1-170):mNG2 (PLCD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only