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We narrowed to 7,986 results for: 104

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Showing: 101 - 150 of 7986 results

  1. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available Since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104567-plasmid-map-sequence-id-201429

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  2. p-dCas9-TET1-Hygro

    Plasmid
    #104404
    Purpose
    transient expression of dCas9-TET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    TET1 (TET1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104404-plasmid-map-sequence-id-204454

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  5. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  6. pHH0103 YAP1 WW domain #2

    Plasmid
    #104228
    Purpose
    Bacterial expression of WW domain #2 from YAP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    YAP1 WW domain #2 (YAP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104228-plasmid-map-sequence-id-204296

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  7. pHH0103 YAP1 WW domain #1

    Plasmid
    #104227
    Purpose
    Bacterial expression of WW domain #1 from YAP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    YAP1 WW domain #1 (YAP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104227-plasmid-map-sequence-id-201508

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  12. pYZ205

    Plasmid
    #104586
    Purpose
    Express SNAP-6His-CBD
    Depositor
    New England Biolabs, Yinhua Zhang
    Insert
    SNAP-6His-CBD
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    104586-plasmid-map-sequence-id-266802

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  29. pBAF

    Plasmid
    #104152
    Purpose
    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.
    Depositor
    Robert Craigie
    Article
    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95
    Insert
    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    Tags
    N-terminal His tag with thrombin cleavage site
    Expression
    Bacterial
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    104152-plasmid-map-sequence-id-201395

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  30. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    104910-plasmid-map-sequence-id-206061

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  32. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104406-plasmid-map-sequence-id-204465

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  36. pQE80L-IL2 C125S

    Plasmid
    #104348
    Purpose
    Expresses human IL-2 (C125S)
    Depositor
    Alexander McLellan
    Insert
    Human IL-2 (C125S) (IL2 Human)
    Tags
    MRGSHHHHHGS-tag
    Expression
    Bacterial
    Mutation
    C125S mutation (TGT -> TCC)
    Promoter
    T5
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104348-plasmid-map-sequence-id-201216

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  41. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  46. pTP261

    Plasmid
    #104166
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling
    Depositor
    Dirk Görlich
    Article
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    1x Cysteine located at bps 493-495 in full sequen…
    Available Since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    104166-plasmid-map-sequence-id-200075

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  49. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

    Plasmid
    #104491
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP7s
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104491-plasmid-map-sequence-id-200288

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Showing: 101 - 150 of 7986 results