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Showing: 871 - 900 of 5867 results

  3. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid
    #203653
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203653-plasmid-map-sequence-id-402484

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  9. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Article
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Use
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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  17. pAAV2-CAG-3xNLS-mScarlet

    Plasmid
    #191098
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191098-plasmid-map-sequence-id-380124

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  20. A3Ai-Cas9n-UGI-NLS

    Plasmid
    #109425
    Purpose
    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109425-plasmid-map-sequence-id-212947

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Showing: 871 - 900 of 5867 results