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170,823 results

Showing: 11961 - 12000 of 170823 results

  3. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171632-plasmid-map-sequence-id-336487

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  4. pS3

    Plasmid
    #50596
    Purpose
    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5
    Depositor
    Jay Keasling
    Article
    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8
    Inserts
    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    50596-plasmid-map-sequence-id-77162

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  9. mCherry-Calreticulin-N-16

    Plasmid
    #55006
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calreticulin (CALR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55006-plasmid-map-sequence-id-470324

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  12. pREP-NFAT2

    Plasmid
    #11788
    Depositor
    Anjana Rao
    Insert
    NFAT2 (NFATC1 Human)
    Expression
    Mammalian
    Available Since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11788-plasmid-map-sequence-id-323906

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  13. TFORF2239

    Plasmid
    #143579
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GATA5 (GATA5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143579-plasmid-map-sequence-id-400353

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  15. pET28a hMDH2

    Plasmid
    #204230
    Purpose
    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH2 (MDH2 Human)
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204230-plasmid-map-sequence-id-413333

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  23. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Article
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Human, Codon optimised)
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169815-plasmid-map-sequence-id-334563

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  28. TFORF1069

    Plasmid
    #143463
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFATC2 (NFATC2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143463-plasmid-map-sequence-id-397913

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  34. pvPE-V4-Puro

    Plasmid
    #240290
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V4-Puro
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240290-plasmid-map-sequence-id-488804

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  36. pTripZ EV

    Plasmid
    #209071
    Purpose
    DOX-ON inducible expression vector that has a stuffer sequence after an LR reaction and used as empty expression vector control
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Insert
    stuffer DNA sequence after LR recombination with pTripZ NEO DEST to be used as an empty vector control
    Use
    Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    209071-plasmid-map-sequence-id-412293

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Showing: 11961 - 12000 of 170823 results