165,075 results

1. pDONR221-SLCO2B1_STOP

   Plasmid

   #161032

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLCO2B1 (SLCO2B1 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCold CL7-Cas9

   Plasmid

   #124890

   Purpose

   obtaining full Cas9 RNP

   Depositor

   Yi Liu

   Article

   Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti PGK Blast V5-LUC (w528-1)

   Plasmid

   #19166

   Purpose

   3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTMVi-M

   Plasmid

   #118757

   Purpose

   Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.

   Depositor

   Jose-Antonio Daros

   Article

   Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

   Insert

   Tobacco mosaic virus cDNA fragment with a cloning polylinker
   

   Use

   Intermediate to build tobacco mosaic virus recomb…

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15bHis6Tnp

   Plasmid

   #79807

   Purpose

   Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

   Depositor

   Keji Zhao

   Article

   Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

   Insert

   His6Tnp
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVT-eeBxb1

   Plasmid

   #232748

   Purpose

   IVT template for making modRNA encoding eeBxb1

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Mutation

   V74A, E229K, V375I

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORANGE GFP-Grin2b KI

   Plasmid

   #131487

   Purpose

   Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2b Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5-ActRIIB

   Plasmid

   #13501

   Depositor

   Jeff Wrana

   Article

   Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

   Insert

   Activin receptor IIB (Acvr2b Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pOSV00170

    Plasmid

    #222934

    Purpose

    Constitutive GFP expression for Bacillus subtilis

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

    Insert

    GFP(Sp)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Phyperspank

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEBB-SBP-HA

    Plasmid

    #36100

    Depositor

    Ezra Burstein

    Type

    Empty backbone

    Tags

    HA and SBP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-Neo-mtkeima

    Plasmid

    #210338

    Purpose

    Overexpression of mtkeima

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mtkeima
    

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CDK12 gRNA (BRDN0001147156)

    Plasmid

    #75915

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK12

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK12 (CDK12 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. #1.4 pre-R-SynapShot-TagBFP

    Plasmid

    #214719

    Purpose

    Expresses red #1.4 pre-SynapShot and TagBFP

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #1.4 pre-R-SynapShot
    

    Use

    AAV

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRISPR-StAR4B (Lenti)

    Plasmid

    #222691

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCMV Puro DEST ERKKTRClover

    Plasmid

    #59150

    Purpose

    Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.R_TRE_minPro

    Plasmid

    #211517

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    SV40 and minPro

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-p150Glued

    Plasmid

    #36154

    Depositor

    David Stephens

    Article

    Watson et al J Cell Sci. 2006 Jul 1;119(Pt 13):2758-6

    Insert

    p150Glued (DCTN1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    see documentation

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PMP34-mCherry-EGFP-Tet-On

    Plasmid

    #109017

    Purpose

    Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    PMP34 (SLC25A17 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRE-Tn7-151

    Plasmid

    #118565

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #160389

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection

    Depositor

    John McCusker

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Cen/ars

    Expression

    Yeast

    Promoter

    Endogenous ATG8 promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. tdTomato-C1

    Plasmid

    #54653

    Purpose

    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    tdTomato

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. Lyn-R-GECO1

    Plasmid

    #120410

    Purpose

    Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.

    Depositor

    Won Do Heo

    Article

    Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11

    Insert

    Lyn-R-GECO1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-EF1α-FLEX-rc[Chronos-GFP]

    Plasmid

    #62725

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pF1-Hs Cdk1/cyclin B1/Cks1

    Plasmid

    #177329

    Purpose

    Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
    

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Promoter

    p10 and polh

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pINTO-NFH::hSNRNP70

    Plasmid

    #65926

    Purpose

    Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Insert

    small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Two silent mutations

    Promoter

    CMV/TO and CMV/TetO2 (T-REx)

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. pXJ-Nanoluc (Nluc)

    Plasmid

    #232656

    Purpose

    Nanoluc luciferase expression driven CMV promoter

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    Nanoluc
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGEX-6p-2-EWSR1

    Plasmid

    #46384

    Depositor

    Heinz Gehring

    Article

    Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

    Insert

    EWSR1 (EWSR1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pBluescriptKS(-)-FRB

    Plasmid

    #104475

    Purpose

    Plasmid carrying FRB with C-terminal HA tag.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    FRB (MTOR Human)
    

    Use

    Phagemid

    Tags

    HA tag

    Mutation

    includes only the FRB domain

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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31. pdCas9-J23111

    Plasmid

    #113149

    Purpose

    Plasmid constitutively expressing dCas9 protein

    Depositor

    Chong Zhang

    Article

    Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

    Insert

    dCas9
    

    Expression

    Bacterial

    Mutation

    D10A & H840A

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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32. pBabe-Puro-BRAF-V600E

    Plasmid

    #15269

    Purpose

    Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    BRAF (BRAF Human)
    

    Use

    Retroviral

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Valine 600 to Glutamate (mutation has been found …

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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33. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mRFP-Ub

    Plasmid

    #11935

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin (RBX1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

    Plasmid

    #179002

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTXB1-pA-DddA11_NT

    Plasmid

    #232443

    Purpose

    ProteinA-DddA11_NT for D&Dseq (sc TFs molecular footprint)

    Depositor

    Ivan Raimondi

    Article

    Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

    Insert

    pA-DddA_NT
    

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CDK12 gRNA (BRDN0001147294)

    Plasmid

    #75916

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK12

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK12 (CDK12 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-YTB

    Plasmid

    #26721

    Depositor

    Edward Callaway

    Article

    Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

    Insert

    venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Rabies virus (strain SAD B19), Chicken)
    

    Expression

    Mammalian

    Available Since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKL2351

    Plasmid

    #199721

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Expression

    Plant

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV 4xSTAR-mNeonGreen-NLS-blast

    Plasmid

    #136258

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge