174,131 results

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pBR322-Pdp1_NTD-Cre

Plasmid

#198274

Purpose

For loading Cre into PVCs; also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-Giantin_C1

Plasmid

#189773

Purpose

Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-Giantin

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCW empty vector

Plasmid

#184708

Purpose

Lentiviral empty vector for doxycycline-inducible expression

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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Gi2-CASE

Plasmid

#168121

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)

Use

Luciferase

Tags

cpVenus on GNG2

Expression

Mammalian

Mutation

NLuc is inserted at C112/E115 within GNAI2

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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G13-CASE

Plasmid

#168127

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)

Use

Luciferase

Tags

cpVenus on GNG9

Expression

Mammalian

Mutation

NLuc is inserted at F125/D126 within GNA13

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCB2.0

Plasmid

#164604

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

hSyn

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMK265 (RAD21-mAC Hygro)

Plasmid

#140539

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-mito-Citron1

Plasmid

#134305

Purpose

mitochondria-targeted direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC(elavl3)-CaMPARI2

Plasmid

#137185

Purpose

pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA

Use

Tol2 plasmid for zebrafish

Tags

FLAG, HA, myc

Promoter

HuC(elavl3)

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-beta-HA-BRCA2

Plasmid

#133553

Purpose

Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

Depositor

David Livingston

Article

Chen et al Mol Cell. 1998 Sep;2(3):317-28.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGAS

Plasmid

#127161

Purpose

expression of human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0284 (pQCascade_entry)

Plasmid

#130635

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP670nano

Plasmid

#127438

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

H2B-miRFP670nano (H2BC11 Human, Synthetic)

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-BI

Plasmid

#126475

Purpose

High-copy number vector for the co-expression of two genes in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pB-rtTA

Plasmid

#126034

Purpose

PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

Depositor

Mauro Calabrese

Article

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Insert

rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735

Use

Piggybac

Expression

Mammalian

Promoter

hUbiC

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3

Plasmid

#116735

Purpose

Lentiviral expression of ERBB3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_12x-601_MA1

Plasmid

#114358

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA1

Use

Unspecified

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Bxb1o_nlsFlag

Plasmid

#119901

Purpose

Expresses Bxb1 integrase in mammalian cells

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

Mammalian codon optimized Bxb1 integrase

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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VN-alpha Synuclein

Plasmid

#89470

Purpose

expresses human alpha Synuclein fused to the N-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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