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Plasmid#173842PurposeA knockout vector for the dog Tgfa.DepositorInsertA gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-hyg2-dEreg
Plasmid#173843PurposeA knockout vector for the dog Ereg.DepositorInsertA gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-puro-dEgfr
Plasmid#173844PurposeA knockout vector for the dog Egfr.DepositorInsertA gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8116 LentiCRISPR v2 sgPTPN14-4
Plasmid#163316PurposeExpresses Cas9 and a human PTPN14-targeting control guide RNADepositorInsertsgPTPN14-4
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8115 LentiCRISPR v2 sgPTPN14-3
Plasmid#163314PurposeExpresses Cas9 and a human PTPN14-targeting control guide RNADepositorInsertsgPTPN14-3
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPRi-CENPB-g10
Plasmid#120218PurposeCENPB CRISPRi guide RNA 10DepositorInsertCENPB CRISPRi guide RNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SCAF4_CRISPR resistant
Plasmid#122465PurposepDONR for Gateway cloningDepositorInsertSCAF4 CRISPR resistant (SCAF4 Human)
UseGateway cloningTagsExpressionMutationSynonymous mutations conferring resistance to gRN…PromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-compact
Plasmid#99369PurposeExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_0387_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2
Plasmid#116999PurposeExpresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertRivularia sp. PCC 7116 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0388_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2_RC
Plasmid#117000PurposeExpresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2 RC, compatible with SENECA acquisition readoutDepositorInsertRivularia sp. PCC 7116 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0386_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1_RC
Plasmid#116998PurposeExpresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertRivularia sp. PCC 7116 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0347_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1
Plasmid#116959PurposeExpresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertCampylobacter fetus subsp. Fetus RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0348_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1_RC
Plasmid#116960PurposeExpresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertCampylobacter fetus subsp. Fetus RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR V2 sgMTHFD1L_2
Plasmid#106317PurposeExpress Cas9 and sgRNA targeting MTHFD1LDepositorInsertsgRNA targeting MTHFD1L
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 1
Plasmid#70651PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 2
Plasmid#70650PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 2
Plasmid#70653PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen1)
Plasmid#73497PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry markerDepositorInsertsdCas9-KRAB-P2A-mCherry
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen2)
Plasmid#73498PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertsdCas9-KRAB
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
OpenCRISPR-1 sgRNA-16nt stem
Plasmid#221566PurposeExpress OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.DepositorInsertOpenCRISPR-1 sgRNA 16nt stem loop
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits