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We narrowed to 25,128 results for: crispr

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Showing: 601 - 620 of 25128 results

  1. pC0052-REPAIR non-targeting guide clone into pC0043

    Plasmid
    #103868
    Purpose
    Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    non-targeting guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103868-plasmid-map-sequence-id-199182

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  2. pRT029

    Plasmid
    #127969
    Purpose
    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127969-plasmid-map-sequence-id-398131

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  6. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60231-plasmid-map-sequence-id-417804

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  8. pC035 - dLwCas13a-msfGFP

    Plasmid
    #91925
    Purpose
    Expresses catalytically inactive LwCas13a for mammalian RNA targeting
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLwCas13a
    Use
    CRISPR
    Tags
    NLS-3xHA and NLS-msfGFP
    Expression
    Mammalian
    Mutation
    R488A and R1060A
    Promoter
    EF-1a
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91925-plasmid-map-sequence-id-228361

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  11. pDF0159 pCMV - huDisCas7-11 mammalian expression

    Plasmid
    #172507
    Purpose
    Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Publication
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV - huDisCas7-11 mammalian expression
    Expression
    Mammalian
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172507-plasmid-map-sequence-id-344930

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  17. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89308-plasmid-map-sequence-id-425605

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  19. pAC93-pmax-dCas9VP160

    Plasmid
    #48225
    Purpose
    dCas9VP160 on pmax expression vector
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR
    Tags
    HA Tag and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48225-plasmid-map-sequence-id-201375

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Showing: 601 - 620 of 25128 results