We narrowed to 67,891 results for: TOR

1. pcDNA3.1-Flag-gp130dimer

   Plasmid

   #174394

   Purpose

   Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   IL6ST cytosolic domain with leucine zipper & C-C linker
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGal4x5-E4T-G210

   Plasmid

   #171077

   Purpose

   The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4-E4-Gless-cassette
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-JEDI-1P-Kv-WPRE

   Plasmid

   #202612

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-Kv
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrichoGate-16

   Plasmid

   #160716

   Purpose

   T-nos Terminator bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   nopaline synthase terminator (T-nos) bordered by BsaI restriction sites
   

   Use

   Synthetic Biology

   Mutation

   Incorporation of BsaI restriction sites

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PL-SIN-PGK-EiP

   Plasmid

   #21312

   Purpose

   Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_PGK_SNIPR_CD8alpha variant 4

   Plasmid

   #188364

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_SNIPR_CD8alpha variant 2, G318A

   Plasmid

   #188365

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK_SNIPR_hsNotch1 LNR

   Plasmid

   #188336

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_PGK_SNIPR_CD8alpha variant 2, V319A

   Plasmid

   #188366

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_V319A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_V319A_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGoldenGreenGate-M (pGGG-M)

    Plasmid

    #165422

    Purpose

    A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Level 2 golden gate (moclo) du…

    Promoter

    Contain GG cassette and MCS with LacZ for blue/wh…

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330_DRS-1 sgRNA / hSpCas9

    Plasmid

    #172841

    Purpose

    Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-DRD2

    Plasmid

    #24099

    Depositor

    Jean-Michel Arrang

    Article

    Jeanneteau et al Mol Biol Cell. 2004 Feb . 15(2):696-705.

    Insert

    DRD2 (DRD2 Human)
    

    Tags

    alpha7 nicotinic signal peptide and eGFP

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-metap2

    Plasmid

    #100512

    Purpose

    Expresses HA tagged metap2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. dRT-pMDLg/pRRE

    Plasmid

    #60488

    Purpose

    gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)

    Depositor

    Boris Fehse

    Article

    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

    Insert

    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
    

    Mutation

    changed Asp at position 249 + 250 to Val

    Available Since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. hGip-R

    Plasmid

    #14942

    Depositor

    Bernard Thorens

    Article

    Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

    Insert

    Gip-R (GIPR Human)
    

    Expression

    Mammalian

    Mutation

    Short form of the receptor

    Available Since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pRevTRE MKL1

    Plasmid

    #19846

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pFloat.T7_v1

    Plasmid

    #133070

    Purpose

    Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3xFLAG-MKL1

    Plasmid

    #11978

    Purpose

    For mammalian expression of human MKL1 with a 3x FLAG tag

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N579S T716A

    Promoter

    CMV

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pES005 (pTet-qacR wt/pQacA-deGFP)

    Plasmid

    #69034

    Purpose

    reporter with wild-type qacR on single plasmid

    Depositor

    Richard Murray

    Article

    de Los Santos et al ACS Synth Biol. 2015 Aug 19.

    Inserts

    qacR (qacR Synthetic)
    deGFP
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    pQacA and pTet

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFA6a-GFP(S65T)-His3MX6

    Plasmid

    #41598

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEGFP.N1-HDAC6.DC (catalytic deficient)

    Plasmid

    #36189

    Depositor

    Tso-Pang Yao

    Article

    Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

    Insert

    HDAC6.DC (HDAC6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    H216/611A

    Promoter

    CMV

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3 GATA1

    Plasmid

    #85693

    Purpose

    Mammalian expression of mouse GATA1

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pME-nls-sfGFP

    Plasmid

    #132973

    Purpose

    full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    nuclear localized super folder GFP
    

    Use

    Gateway vector

    Tags

    6x myc and SV40 NLS

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-nef-CIAO2-TVA-mCherry

    Plasmid

    #149297

    Purpose

    Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    TVA:mCherry
    

    Use

    AAV

    Mutation

    Start codon removed.

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET41a iGluu

    Plasmid

    #119834

    Purpose

    Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluu
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S72T

    Promoter

    T7

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBK1289-AAV-dCjCas9

    Plasmid

    #223138

    Purpose

    AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3 Barr2 flag

    Plasmid

    #14685

    Depositor

    Robert Lefkowitz

    Article

    Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

    Insert

    beta-arrestin 2 (Arrb2 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pHR_PGK_SNIPR_CD28 SNIPR

    Plasmid

    #188358

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-JEDI-1P-WPRE

    Plasmid

    #202613

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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