165,971 results

1. pcDNA3 HA-Sumo2 WT

   Plasmid

   #48967

   Purpose

   Expresses HA SUMO2 Wild Type in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO2 (SUMO2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS117 His14-Avi-45xGS-anti GFP nanobody

   Plasmid

   #199370

   Purpose

   Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-GFP nanobody
   

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-Luc-mCherry-puro

   Plasmid

   #183685

   Purpose

   Lentiviral vector expressing luciferase and mCherry.

   Depositor

   Andrew Kung

   Article

   Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

   Insert

   Luc-E2A-mCherry-T2A-PuroR
   

   Use

   Lentiviral

   Promoter

   Human Ubiquitin C (UbC) promoter

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUGW-Golgi-TurboID

   Plasmid

   #200964

   Purpose

   3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   Golgi-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. evoBxb1

   Plasmid

   #222338

   Purpose

   Plasmid for expressing evolved Bxb1 in the mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved Bxb1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. UBC-4xmNG-dMCP

   Plasmid

   #231093

   Purpose

   Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).

   Depositor

   John Ngo

   Article

   Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

   Insert

   4xmNG-dMCP
   

   Expression

   Mammalian

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX_DSB_Spectrum-V1

   Plasmid

   #192474

   Purpose

   Lentiviral DSB Spectrum V1 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG/MNase

   Plasmid

   #123461

   Purpose

   Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

   Depositor

   Steven Henikoff

   Article

   Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

   Insert

   Protein A-Protein G-Micrococcal Nuclease
   

   Tags

   6xHIS and HA

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-EGFP-CNA35

   Plasmid

   #61603

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with EGFP

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   EGFP and CNA35
   

   Tags

   His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-6xHis [N144/14R]

    Plasmid

    #177500

    Purpose

    Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-6xHis recombinant mouse monoclonal antibody
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-TO-dCas9

    Plasmid

    #75381

    Purpose

    dCas9

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-bacteria

    Plasmid

    #44249

    Purpose

    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 (bacteria)
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-N EBNA1

    Plasmid

    #37954

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    EBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-DJ8R

    Plasmid

    #213974

    Purpose

    AAV packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Neurosci Bull. 2023 Jul 11. doi: 10.1007

    Type

    Empty backbone

    Use

    AAV

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pATetO 6XHis-Phusion

    Plasmid

    #206210

    Purpose

    Inducible expression of DNA polymerase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

    Insert

    Phusion DNA polymerase
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LAMP1-mGFP

    Plasmid

    #34831

    Depositor

    Esteban Dell'Angelica

    Article

    Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

    Insert

    LAMP1 (LAMP1 Human)
    

    Tags

    GSTGSTGSTGA linker, Kozak, and mGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBad-LgBiT-PhoCl1-SmBiT-MBP

    Plasmid

    #164034

    Purpose

    luciferase-based PhoCl screening construct

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    LgBiT-PhoCl1-SmBiT-MBP
    

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Promoter

    araBad promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1 Lifeact-EGFP

    Plasmid

    #58470

    Purpose

    Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    Lifeact
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS598 EF1alpha-EGFPcre

    Plasmid

    #11923

    Purpose

    Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. EB1-tdTomato

    Plasmid

    #50825

    Purpose

    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Insert

    EB1 (Mapre1 Mouse)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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