163,606 results

1. pX330S-2

   Plasmid

   #58778

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSSRB069_pAC8-hsDDB1dB

   Plasmid

   #218790

   Purpose

   Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Insect

   Mutation

   Deleted amino acids 396-705 and replaced with GNG…

   Promoter

   polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM464 (deletions)

   Plasmid

   #108322

   Purpose

   Orbit integrating plasmid to delete target gene.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   Bxb1 attB site
   

   Use

   Orbit integration plasmid for creating knockouts …

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYD1

   Plasmid

   #73447

   Purpose

   expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

   Depositor

   Dane Wittrup

   Article

   Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

   Type

   Empty backbone

   Use

   Tags

   6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-IRES-Hygro

   Plasmid

   #85134

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

   Plasmid

   #210525

   Purpose

   AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mtagBFP-KASH
   U6-gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRIS-PITChv2-Puro-dTAG (BRD4)

   Plasmid

   #91793

   Purpose

   PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   Puro-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a-hMLH1dn

   Plasmid

   #174824

   Purpose

   Mammalian expression of human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (codon optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY093-EFS-BCMA-Blast-WPRE

   Plasmid

   #192194

   Purpose

   Lenti-BCMA-OE-Blast

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-SFFV-FLAG-SQSTM1

    Plasmid

    #223715

    Purpose

    Expresses Flag-tagged SQSTM1

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. SpyCatcher003 S49C

    Plasmid

    #133448

    Purpose

    Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Contains S49C mutation

    Promoter

    T7

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_Gal4UAS_tBFP_PGK_mCherry

    Plasmid

    #79130

    Purpose

    Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    tBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP VAMP7 (1-220)

    Plasmid

    #42316

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 (1-220) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMP1039

    Plasmid

    #119239

    Purpose

    expresses Tn7 transposase

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEcgRNA

    Plasmid

    #166581

    Purpose

    CRISPR-Cas9-assisted genome editing in Escherichia coli

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-CMV-3xFLAG-gateway-EGFP

    Plasmid

    #122845

    Purpose

    N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM512

    Plasmid

    #140192

    Purpose

    For curing of Orbit-integrated plasmids and associated drug marker

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cloned in gp47 directinality factor in place of R…

    Promoter

    EM7 and Ptet

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_BE4max_P2A_GFP

    Plasmid

    #112099

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKM461

    Plasmid

    #108320

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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