20,193 results
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Plasmid#124110PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only
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MP6
Plasmid#69669PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1
ExpressionBacterialPromoterE. coli PBADAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TATA-GFP
Plasmid#110833Purposenegative controlDepositorInsertGFP
UseLentiviralExpressionMammalianAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianPromoterSFFVAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSL1142 (pSPIN, pBBR1 backbone)
Plasmid#160730PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.Luc.IRES.EGFP.SV40
Plasmid#105533PurposeAAV expression of Luciferase and EGFP from CMV promoterDepositorInsertLuciferase
UseAAV and LuciferaseTagsIRES-GFPExpressionMammalianPromoterCMVAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-EEA1 wt
Plasmid#42307DepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
ER-GA
Plasmid#209871PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddGFP A
TagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#126217PurposeLabeling of inhibitory synapses (green fluorescence) in cre-expressing neuronsDepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAV and Cre/LoxExpressionMammalianPromoterEF1AAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mTagBFP2
Plasmid#99267PurposeContains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmTagBFP2-3XHA
TagsH2BExpressionMammalianPromoterSP6Available SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti U6-sgRNA/EF1a-mCherry
Plasmid#114199PurposeLentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoterDepositorInsertsgRNA scaffold
UseLentiviralTagsmCherryExpressionMammalianPromoterU6Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-ultraID
Plasmid#172879Purposeexpresses ultraID in E. coliDepositorInsertultraID
TagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMG36E
Plasmid#133853PurposeExpression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterP32Available SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro
Plasmid#188768PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#209723PurposeExpresses mitochondrially targeted low affinity ratiometric ATP sensorDepositorInsert(mito).iATPSnFR2.A95A.A119L.miRFP670nano3
UseAAVExpressionMammalianPromoterCAGAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HsPeg10rc3
Plasmid#174859PurposeExpresses recoded human Peg10 in mammalian cellsDepositorAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-DEST
Plasmid#80490PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)ExpressionMammalianAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-hTERT
Plasmid#1771PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorAvailable SinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
4xNFkB Luc
Plasmid#111216Purposereporter geneDepositorInsertfirefly Luciferase
ExpressionMammalianAvailable SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-cOVA-IRES-BFP
Plasmid#135074PurposeExpresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.DepositorAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-MCS
Plasmid#80488Purposedonor vector for AAVS1 targeting (puromycin selection) with multiple cloning siteDepositorTypeEmpty backboneUseDonor vector (human)ExpressionMammalianAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3basic luciferase and renilla_polyA
Plasmid#128046PurposepGL3 basic luciferase reporter with co-expression of renilla luciferaseDepositorTypeEmpty backboneExpressionMammalianAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_TurboID_FLAG_Nt
Plasmid#194072Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-TurboID-FLAGExpressionMammalianAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bARGSer-AxeTxe
Plasmid#192473PurposeSecond-generation bacterial acoustic reporter gene derived from SerratiaDepositorInsertsbARGSer
AxeTxe
ExpressionBacterialMutationthe gene Ser39006_001280 was deleted from the clu…PromoterNative promoter from Enterococcus faecium and pBADAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScaf
Plasmid#111401PurposePhagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18DepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-GFP
Plasmid#51463PurposeIs a biosensor for PIP3DepositorAvailable SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRKH3-ermGFP
Plasmid#27169DepositorInserterythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
ExpressionBacterialMutationpresence of an additional 183-bp region at N term…Available SinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMA122 - peel-1 negative selection
Plasmid#34873PurposeNegative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegansDepositorInsertpeel-1 (peel-1 Nematode)
UseWorm targetingAvailable SinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
UBC-4xmNG-dMCP
Plasmid#231093PurposeEncodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).DepositorInsert4xmNG-dMCP
ExpressionMammalianAvailable SinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-F-del Casp9.IRES.GFP
Plasmid#15567DepositorInsertFKBP12(V36)-p30Caspase9 (FKBP1A Human)
UseRetroviralTagsFKBP12 and HA epitopeExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lac…Available SinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAV SYN flex PSAM4 GlyR IRES EGFP
Plasmid#119741PurposeCre-dependent chemogenetic inhibitor expressionDepositorHas ServiceAAV5 and AAV9InsertPSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
UseAAVExpressionMammalianMutationL131G, Q139L, Y217FPromotersynapsinAvailable SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-TFEB
Plasmid#38119PurposeMammalian expression of Transcription factor EB fused to EGFPDepositorAvailable SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit HC
Plasmid#221355PurposeMammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit HC; to be used with Anti-Strep [IPI-C23.21] light chain (Plasmid 221356) to make the antibody.DepositorInsertAnti-Strep [IPI-C23.21] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
Plasmid#221356PurposeMammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa; to be used with Anti-Strep [IPI-C23.21] heavy chain (Plasmid 221355) to make the antibody.DepositorInsertAnti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMV306DIhsp+LuxG13
Plasmid#49999PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertBacterial luciferase operon + G13 promoter
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterG13 from M. marinum G13 promoter driving luxCAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mito-B
Plasmid#209864PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP B
TagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3m
Plasmid#208698PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neuronsDepositorHas ServiceAAV1InsertGPCR activation based dopamine (DA) sensor GRAB_gDA3m
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD
Plasmid#204359PurposeAAV vector for coexpression of miniDq and KORD under the control of human synapsin promoterDepositorInsertminiDq-mCherry-P2A-HA-KORD
UseAAVTagsHA (for KORD) and mCherry (for miniDq)MutationFor miniDq, the third intracellular loop (ICL3) o…PromoterhSynAvailable SinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only