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We narrowed to 1,640 results for: plasmid for e coli

Showing: 1601 - 1640 of 1640 results

  1. B8-pVTCB052

    Plasmid
    #231302
    Purpose
    Plasmid used for expression of AI-designed GFP protein B8 in E. coli
    Depositor
    Alexander Rives
    Insert
    B8
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231302-plasmid-map-sequence-id-463621

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  2. pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

    Plasmid
    #191651
    Purpose
    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4
    Depositor
    Emily Balskus
    Article
    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:
    Insert
    CymR
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    191651-plasmid-map-sequence-id-375980

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  3. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

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  4. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234454
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234454-plasmid-map-sequence-id-465691

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  5. esmGFP-C10-pVTCB052

    Plasmid
    #231303
    Purpose
    Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli
    Depositor
    Alexander Rives
    Insert
    esmGFP-C10
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231303-plasmid-map-sequence-id-463619

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  8. HIS_TEV_hsSYX3

    Plasmid
    #179290
    Purpose
    E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7lac
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179290-plasmid-map-sequence-id-354913

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  16. pHis-TEV-Nsp15-E266Q

    Plasmid
    #218803
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    E266Q (G to C)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218803-plasmid-map-sequence-id-433302

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  17. pHis-TEV-Nsp15-D183N

    Plasmid
    #219526
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    D183N (G to A)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219526-plasmid-map-sequence-id-433305

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  18. pHis-TEV-Nsp15-H234A

    Plasmid
    #219529
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    H234A (CAC to GCG)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219529-plasmid-map-sequence-id-433314

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  19. pHis-TEV-Nsp15-V66L

    Plasmid
    #219524
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    V66L (G to C)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219524-plasmid-map-sequence-id-433377

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  20. pHis-TEV-Nsp15-A81V

    Plasmid
    #219525
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    A81V (C to T)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219525-plasmid-map-sequence-id-433375

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  21. pGST-HsSyx-375-792

    Plasmid
    #179292
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + GST + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179292-plasmid-map-sequence-id-354918

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  22. pSUMO-HsSyx3

    Plasmid
    #179288
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179288-plasmid-map-sequence-id-354924

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  23. pET-hMICU1EF

    Plasmid
    #199180
    Purpose
    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
    Depositor
    Ming-Feng Tsai
    Article
    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12
    Insert
    MICU1 (MICU1 Human)
    Use
    Tags
    His6 and MBP, Thrombin site, TEV site
    Expression
    Mutation
    D421A, E432K, F433W, F453W
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    199180-plasmid-map-sequence-id-403144

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  24. pGFP-HsSyx3

    Plasmid
    #179289
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + GFP + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    179289-plasmid-map-sequence-id-354922

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  25. pHsSyx3-MBP

    Plasmid
    #179286
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    TEV protease cleavage site + mature maltose bindi…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179286-plasmid-map-sequence-id-354920

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  26. pET3a-SF-LMNC-TS

    Plasmid
    #169374
    Purpose
    E. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    LMNA residues 31-542 (LMNA Human)
    Use
    Tags
    Split-FlAsH Tag and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    deleted amino acid 1-30 (unstructured head domain…
    Promoter
    Native pET3a promotor
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169374-plasmid-map-sequence-id-333772

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  29. pNIC-ZB-SPRTN-Y117C-FL

    Plasmid
    #110219
    Purpose
    Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.
    Depositor
    Kristijan Ramadan
    Article
    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    His6 and ZB
    Expression
    Bacterial
    Mutation
    Y117C, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110219-plasmid-map-sequence-id-210947

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  30. 7AA-aSyn-pRK172

    Plasmid
    #236197
    Purpose
    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.
    Depositor
    Yang Yang, Sjors Scheres
    Article
    Yang et al Acta Neuropathol. 2023 May;145(5):561-57
    Insert
    SNCA (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion os MAAAEKT at residue 22
    Promoter
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236197-plasmid-map-sequence-id-469796

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  31. pMBP-HsSyx3

    Plasmid
    #179287
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179287-plasmid-map-sequence-id-354925

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  32. pMBP-HsSyx-375-792

    Plasmid
    #179291
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179291-plasmid-map-sequence-id-354915

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  33. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Use
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Promoter
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

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  37. pHis6-SUMO-g0s2

    Plasmid
    #179293
    Purpose
    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Use
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    T7
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179293-plasmid-map-sequence-id-354916

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  38. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Richard C. Willson
    Article
    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    122462-plasmid-map-sequence-id-243544

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  39. MBP-g0s2

    Plasmid
    #179294
    Purpose
    E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Use
    Tags
    mature maltose binding protein + enterokinase cle…
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    Ptac
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179294-plasmid-map-sequence-id-354917

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  40. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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Showing: 1601 - 1640 of 1640 results