19,598 results

1. M51 Super 8x FOPFlash (TOPFlash mutant)

   Plasmid

   #12457

   Purpose

   Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   mutant TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190034

   Purpose

   vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Expression

   Mammalian

   Mutation

   A134V,A140V,A141V,A143V

   Promoter

   CBh, SV40, hU6

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyTag003-mKate2

   Plasmid

   #133452

   Purpose

   Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mKate2
   

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEVOL-pBpF

   Plasmid

   #31190

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

   Depositor

   Peter Schultz

   Article

   Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

   Insert

   M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
   

   Expression

   Bacterial

   Mutation

   Y32G, E107P, D158T, I159S

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-JAK1

   Plasmid

   #174572

   Purpose

   Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   JAK1 (JAK1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

   Plasmid

   #126705

   Purpose

   donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   GFP
   

   Use

   CRISPR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS117 His14-Avi-45xGS-anti GFP nanobody

   Plasmid

   #199370

   Purpose

   Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-GFP nanobody
   

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmScarlet3_C1

   Plasmid

   #189753

   Purpose

   C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GW1-PercevalHR

   Plasmid

   #49082

   Purpose

   Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTCF156 HLA-A*02:01

    Plasmid

    #180456

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*02:01
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRIS-PITChv2-Puro-dTAG (BRD4)

    Plasmid

    #91793

    Purpose

    PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    Puro-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TEVSH

    Plasmid

    #125194

    Purpose

    His-tagged TEV protease with solubility increasing mutations

    Depositor

    Helena Berglund

    Article

    van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

    Insert

    TEV_SH
    

    Tags

    V5 His-tag

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLHKII-pGFPN3

    Plasmid

    #21920

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the full length human HKII cDNA sequence …

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRUPATH Triple Gaq

    Plasmid

    #196058

    Purpose

    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. MCP-APEX2

    Plasmid

    #154936

    Purpose

    NLS-V5-MCP-APEX2

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    NLS-V5-MCP-APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAXY0001

    Plasmid

    #179834

    Purpose

    binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

    Insert

    eYGFPuv
    

    Use

    Binary vector for plant transformation

    Expression

    Plant

    Promoter

    35S

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDMyc-TNPO1

    Plasmid

    #86081

    Purpose

    A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-mNeonGreen

    Plasmid

    #99134

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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