19,753 results

1. OpenCRISPR-1 sgRNA-16nt stem

   Plasmid

   #221566

   Purpose

   Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 16nt stem loop
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3-CFP

   Plasmid

   #13030

   Purpose

   Mammalian expression vector for cloning your gene fused to CFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-eGFP-C1-VPS35

   Plasmid

   #163622

   Purpose

   human VPS35 cDNA C-terminally tagged with eGFP

   Depositor

   Josef Kittler

   Article

   Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

   Insert

   human VPS35 (VPS35 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. scAAV-hIBA1a-GFP

   Plasmid

   #214146

   Purpose

   Self-complementary AAV vector packaging GFP under the human Iba1 promoter

   Depositor

   Chun-Li Zhang

   Article

   Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

   Insert

   GFP
   

   Use

   AAV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB-cLUC

   Plasmid

   #174051

   Purpose

   Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

   Depositor

   Alberto Macho

   Article

   Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Plant

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

   Plasmid

   #86674

   Purpose

   Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   GFP-FLAG-cGAS E225A/D227A
   

   Use

   Lentiviral

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcw107

   Plasmid

   #62511

   Purpose

   Gateway Expression Vector

   Depositor

   John Doench, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304

   Plasmid

   #25890

   Purpose

   3rd generation lentiviral Gateway destination vector. Blasticidin selection, V5 tag.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-U6-crRNA-empty

   Plasmid

   #134921

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   crRNA for CRISPR-Cas3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TO-NFIA.1-SOX9

    Plasmid

    #182310

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. beta-catenin (S33Y)-pcw107

    Plasmid

    #64615

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Mutation

    S33Y

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKL2299A

    Plasmid

    #222105

    Purpose

    A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

    Depositor

    Kan Wang

    Article

    Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

    Insert

    virA
    

    Expression

    Bacterial and Plant

    Promoter

    virA promoter

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pinducer 20 DN-KASH

    Plasmid

    #125554

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    tetracycline responsive element

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Expression

    Bacterial

    Promoter

    Rhamnose promoter

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-BirA

    Plasmid

    #20857

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    BirA
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pinducer 20 DN-KASHΔPPPL

    Plasmid

    #129280

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

    Depositor

    Daniel Conway

    Article

    Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Mutation

    the last 4 amino acids (PPPL) of the KASH domain …

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1X-Nup93

    Plasmid

    #87336

    Purpose

    To express human Nup93 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP93 (NUP93 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-All-in-one-hCascade

    Plasmid

    #134919

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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