Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 11,283 results for: Vars;

Showing: 10201 - 10230 of 11283 results

  3. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40

    Plasmid
    #100838
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1 and AAV9
    Insert
    GCaMP6m
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …
    Promoter
    Synapsin
    Available Since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100838-plasmid-map-sequence-id-198300

    Enlarge

  4. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172316
    Purpose
    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172316-plasmid-map-sequence-id-343650

    Enlarge

  8. pJR021

    Plasmid
    #198814
    Purpose
    Variant of yellow fluorescent protein for transient gene expression
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::destabilizedYFP::let-838 3'UTR
    Expression
    Worm
    Mutation
    YFP with destabilized signal from mouse ornithine…
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198814-plasmid-map-sequence-id-391694

    Enlarge

  10. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid
    #114111
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100
    Insert
    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114111-plasmid-map-sequence-id-252509

    Enlarge

  12. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available Since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

    Enlarge

  14. FKBP*-PTEN

    Plasmid
    #86781
    Purpose
    Fusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
    Depositor
    Britta Eickholt
    Article
    An et al PLoS One. 2015 Dec 30;10(12):e0145783. d
    Insert
    PTEN (PTEN Human)
    Tags
    FKBP* (F36V and L106P) and linker
    Expression
    Mammalian
    Available Since
    Feb. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    86781-plasmid-map-sequence-id-169199

    Enlarge

  21. pCX-SponGee-Kras

    Plasmid
    #134777
    Purpose
    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Article
    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e
    Insert
    SponGee-Kras
    Tags
    FLAG and mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    134777-plasmid-map-sequence-id-266270

    Enlarge

  22. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

    Enlarge

  26. pNSSCPG

    Plasmid
    #183187
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Expression
    Bacterial
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183187-plasmid-map-sequence-id-366657

    Enlarge

  27. cfmTurquoise-N

    Plasmid
    #139284
    Purpose
    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.
    Depositor
    Ikuo Wada
    Article
    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d
    Insert
    cfmTurquoise
    Tags
    Multi-cloning sites
    Expression
    Mammalian
    Mutation
    Cys48Ser and Cys70/Met
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    139284-plasmid-map-sequence-id-291944

    Enlarge

Showing: 10201 - 10230 of 11283 results