165,271 results

1. pWOL-GW

   Plasmid

   #209373

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by WOL promoter

   Depositor

   Lucia Strader

   Article

   Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+ Grx1-roCherry

   Plasmid

   #141068

   Purpose

   Fluorescent biosensor for 2GSH/GSSG ratio

   Depositor

   Dmitry Bilan

   Article

   Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

   Insert

   Grx1-roCherry
   

   Expression

   Mammalian

   Mutation

   Changed Alanine 150 to Cysteine, inserted Threon…

   Promoter

   CMV, SP6

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-FR-GECO1a

   Plasmid

   #163681

   Purpose

   Far-red fluorescent calcium indicator FR-GECO1a

   Depositor

   Robert Campbell

   Article

   Dalangin et al bioRxiv 2020.11.12.380089

   Insert

   FR-GECO1a
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFC333

   Plasmid

   #87844

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   ble (bleomycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC008 - pBR322 with tetR-inducible RFP

   Plasmid

   #79157

   Purpose

   inducible RFP expression vector

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   TetR + RFP
   

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_RBM10_WT

   Plasmid

   #81958

   Purpose

   Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   RBM10 (RBM10 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG::Gdnf

   Plasmid

   #78209

   Purpose

   piggybac vector for mouse Gdnf expression

   Depositor

   Ivo Lieberam

   Article

   Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

   Insert

   Gdnf (Gdnf Mouse)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

8. Ngn2

   Plasmid

   #34999

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Neurogenin-2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOpen-EcoRV

   Plasmid

   #165548

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   EcoRV
   

   Use

   Synthetic Biology

   Available Since

   Aug. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBR322-TIMER

    Plasmid

    #103056

    Purpose

    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEmerald-Beta-Catenin-20

    Plasmid

    #54017

    Purpose

    Localization: Tight Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Beta-Catenin (Ctnnb1 Mouse)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. SpCas9-NG-YE1-BE4max

    Plasmid

    #198535

    Purpose

    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1 p37

    Plasmid

    #169015

    Purpose

    Expresses GST-p37 (human, codon optimized for expression in bacteria)

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    p37 (UBXN2B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    sequence was codon optimized for expression in E.…

    Promoter

    tac

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-ALPK1

    Plasmid

    #23630

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. p2R3a-nosT

    Plasmid

    #71272

    Purpose

    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    nopaline synthase terminator
    

    Use

    Gateway

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

    Plasmid

    #29712

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ10530

    Plasmid

    #239266

    Purpose

    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    NES-dPspCas13b-EGFP-ABI
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag tag and V5 tag

    Expression

    Mammalian

    Mutation

    H133A and H1058A in PspCas13b

    Promoter

    EF1a

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

    Plasmid

    #207880

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mKeima-Red-C1

    Plasmid

    #54546

    Purpose

    Localization: C1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKeima-Red

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nsp6-mCherry

    Plasmid

    #165133

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp6 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMXS-GshF-blasticidin

    Plasmid

    #210313

    Purpose

    Expresses humanized Streptococcus thermophilus GshF in mammalian cells

    Depositor

    Kivanc Birsoy

    Article

    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

    Insert

    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    

    Use

    Retroviral

    Tags

    FLAG peptide

    Mutation

    Codon optimized for expression in human cells

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAVS1-P-CAG-GFP

    Plasmid

    #80491

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    CAG-GFP
    

    Use

    Donor vector (human)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. Hsp68-LacZ-Gateway

    Plasmid

    #37843

    Depositor

    Nadav Ahituv

    Article

    Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

    Type

    Empty backbone

    Use

    Mouse Targeting

    Promoter

    Mouse Hsp68 minimal promoter

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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24. pFG7m34GW

    Plasmid

    #133747

    Purpose

    Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

    Depositor

    Niko Geldner

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCBC-DT2T3

    Plasmid

    #50591

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    

    Use

    CRISPR; Pcr template

    Expression

    Plant

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBabe puro HA PIK3CA H1047R

    Plasmid

    #12524

    Purpose

    Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1047R. Tumor-derived allele.

    Available Since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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27. pIS1-Eef25UTR-TOPmut-renilla

    Plasmid

    #38236

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    The first nucleotides of the 5' UTR were cha…

    Promoter

    Endogenous Eef2

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

28. NanoBRET Assay Vector, HaloTag-APC

    Plasmid

    #237145

    Purpose

    Express HaloTag(R)-APC Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    APC (APC Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pDONR221-CTSL

    Plasmid

    #158444

    Purpose

    Gateway Cloning compatible entry vector for the human CTSL gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSL (CTSL Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pNCS-Antares

    Plasmid

    #74279

    Purpose

    Constitutive expression of Antares in bacteria

    Depositor

    Michael Lin

    Article

    Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Antares
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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