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p266

Plasmid

#186185

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 266

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLX401-INK4A

Plasmid

#121919

Purpose

Doxycycline-inducible expression of p16-INK4A product of CDKN2A

Depositor

William Hahn

Article

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

p16-INK4A (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE Tet ON

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSXneo 270(T2AG3)

Plasmid

#12403

Depositor

Titia de Lange

Article

Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

Insert

270(T2AG3)

Expression

Mammalian

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183523

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

I146M/G174S

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-ACSL3-mCherry

Plasmid

#87158

Purpose

Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ACSL3 (ACSL3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMSP12::mCherry

Plasmid

#30169

Depositor

Lalita Ramakrishnan

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

mCherry

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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Active Wnt5B-V5

Plasmid

#43814

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT5B (WNT5B Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-EB3-7

Plasmid

#55037

Purpose

Localization: MT End Binding Protein, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

EB3 (MAPRE3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pmVen-DHX9

Plasmid

#211409

Purpose

mammalian expression of human DHX9 N-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

DHX9 (DHX9 Human)

Tags

mVenus

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

Plasmid

#220735

Purpose

AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.8h.8TM.28.3z

Plasmid

#200670

Purpose

Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

Plasmid

#247160

Purpose

Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP 150TAG

Plasmid

#197574

Purpose

Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
M. alvus Pyl-tRNA (6)

Tags

His6

Expression

Bacterial

Mutation

N150TAG

Promoter

araC and lpp

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCX459

Plasmid

#229214

Purpose

CRISPR array plasmid for MAP1B (Spacers 1-24)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 1-24)

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pKOV

Plasmid

#25769

Depositor

George Church

Article

Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

Plasmid

#100848

Purpose

AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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eGFP-SUN1

Plasmid

#213515

Purpose

Expresses human eGFP-SUN1 in mammalian cells

Depositor

Karim Mekhail

Article

Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319

Insert

SUN1 (SUN1 Human)

Expression

Mammalian

Mutation

SUN1 has a silent mutation on its gene (nucleotid…

Promoter

CMV

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-SPI1

Plasmid

#162824

Purpose

Doxycycline-inducible overexpression of human SPI1

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

SPI1 (SPI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet ON

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-CybSEP2-WPRE

Plasmid

#207661

Purpose

Monitoring neuropeptide release

Depositor

Sung Han

Article

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

Cytochrome b-561 (Cyb561 Mouse)

Use

AAV

Mutation

Histidine 86 and 159 to alanines, inserted 2 X SE…

Promoter

hSynapsin

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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