165,255 results

1. pcDNA3-PhoDer

   Plasmid

   #188473

   Purpose

   Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

   Depositor

   Gary Mo

   Article

   Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

   Insert

   PhoDer (GSDMD Human, Synthetic)
   

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1521 (pSPIN, pSC101* backbone)

   Plasmid

   #160729

   Purpose

   Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-10His-mStayGold (E138D)

   Plasmid

   #211363

   Purpose

   Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   mStayGold
   

   Tags

   10xHis-tag

   Expression

   Mammalian

   Mutation

   E138D

   Promoter

   CMV

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-Blast-PIP-FUCCI

   Plasmid

   #138715

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. v3em-Nterm-PE2max

   Plasmid

   #198734

   Purpose

   AAV genome encoding N-terminal PE2max

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   Nterm PE2max-NpuN
   

   Use

   AAV

   Promoter

   Cbh

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. v3em-Cterm-PE2max-∆RNaseH-dualU6

   Plasmid

   #198735

   Purpose

   AAV genome encoding C-terminal PE2max and U6 expression cassettes

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   NpuC-CtermPE2max∆RNaseH
   

   Use

   AAV

   Promoter

   Cbh

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBMN(CMV-copGFP-Luc2-Puro)

   Plasmid

   #80389

   Purpose

   To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Luc2-F2A-Puromycin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA-Ubiquitin-K6

   Plasmid

   #22900

   Depositor

   Sandra Weller

   Article

   Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K6 only, all other lysines mutated to arginines

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-HA-USP1

    Plasmid

    #22596

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP1 (USP1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNCA

    Plasmid

    #17363

    Depositor

    Stephen Goff

    Article

    Colicelli et al J Mol Biol. 1988 Jan 5. 199(1):47-59.

    Insert

    MMLV proviral DNA
    

    Available Since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-PE7-P2A-BSD

    Plasmid

    #224122

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPLICS Mt-ER- PM Short P2A

    Plasmid

    #164110

    Purpose

    Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM- Mt-ER Short P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNEU-hMbPylRS-4xU6M15

    Plasmid

    #105830

    Purpose

    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

    Depositor

    Irene Coin

    Article

    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

    Inserts

    Humanized MbPylRS
    tRNAM15
    

    Expression

    Mammalian

    Mutation

    (Y271A, Y349F)MbPylRS

    Promoter

    CMV and U6

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247262

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
    

    Expression

    Mammalian

    Mutation

    C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid

    #183532

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Mutation

    None

    Promoter

    nEF

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti.PGK.LifeAct-Ruby.W

    Plasmid

    #51009

    Purpose

    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    LifeAct-mRFPruby
    

    Use

    Lentiviral

    Tags

    LifeAct

    Expression

    Mammalian

    Promoter

    human PGK

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29a-YS14

    Plasmid

    #66890

    Purpose

    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

    Depositor

    Jung-Hyun Min

    Article

    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

    Inserts

    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    

    Tags

    H2A, H2B, H3, H4, His6 tag, and Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Str-Ii_VSVG-SBP-mCherry

    Plasmid

    #65301

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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