We narrowed to 3,043 results for: beta-actin
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Plasmid#196678PurposeAAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.DepositorInsertPKIαmut-P2A-mRuby3
UseAAV and Cre/LoxExpressionMammalianAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-NCreIntN-T2A-mTagBFP2
Plasmid#160500PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-VARNAM2-Kv2.1PR
Plasmid#195536PurposeFlp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM SI linkerPromoterCAGAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA
Plasmid#194247PurposeExpresses 3 miRNAs targeting human alpha-SynucleinDepositorAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-mGreenLantern-KASH
Plasmid#191094PurposeTo express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertmGreenLantern-KASH
UseAAV and Synthetic BiologyTagsmGreenLantern fused to the KASH domain of Nesprin…ExpressionMammalianMutationOptimizzation was done using the Human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-AausFP1
Plasmid#191096PurposeTo express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-AausFP1
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
UPB-pancreas (UPB7)
Plasmid#180787PurposeUnique Projection Barcode (UPB7)-Pancreas for Projection-seqDepositorInserthM4D(Gi) (partial )
UseAAVExpressionMammalianPromoterCAGAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-MTN
Plasmid#184607PurposeFor expression of a fluorescent sensor for melatonin in mammalian cellsDepositorInsertMTRIA-MTN
ExpressionMammalianPromoterCAG promoterAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Electra1-P2A-EGFP
Plasmid#184935PurposeExpresses blue fluorescent protein Electra1 and EGFP in mammalian cellsDepositorInsertElectra1-P2A-EGFP
UseAAVExpressionMammalianPromoterCAGAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
UPB-esophagus (UPB5)
Plasmid#180783PurposeUnique Projection Barcode (UPB5)-Esophagus for Projection-seqDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iGECInano
Plasmid#186188PurposeAAV vector packaging small FRET-based near-infrared genetically-encoded calcium indicatorDepositorInsertiGECInano
UseAAVPromoterCAGAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-NPY
Plasmid#184613PurposeFor expression of a fluorescent sensor for neuropeptide Y in mammalian cellsDepositorInsertMTRIA-NPY
ExpressionMammalianPromoterCAG promoterAvailable SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10871
Plasmid#183962PurposeU6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPRDepositorInsertsdCas12a(wildtype)
poly-crRNA array targeting Sox2-Klf4-Oct4
UseCRISPRTagsHAExpressionMammalianMutationD832APromoterCAG and U6Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10333
Plasmid#183955PurposeCAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertNuclease-dead Lb Cas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832APromoterCAGAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-mKate2-IRES-Hph
Plasmid#183616PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.DepositorInsertmKate2
TagsHuman EMD transmembrane domain and NLSExpressionMammalianPromoterCAGAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GLI1_pLENTI-CAG-IRES-GFP
Plasmid#176992PurposeMammalian lentiviral expression vector encoding GLI1DepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD2529-CAG-HA-mouse GARP
Plasmid#180258PurposeMouse full length GARP expressionDepositorAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PD2529-CAG-Flag-mouse TGFb2
Plasmid#180261PurposeMouse full length TGFβ2 expressionDepositorAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-NCreIntN-EF1a-mTagBFP2
Plasmid#160507PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianPromoterCAGAvailable SinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA
Plasmid#175177PurposeCre-dependent GCaMP6s and mBeRFP expressionDepositorInsertGCaMP6s
UseAAVTagsP2A-mBeRFPPromoterCAGAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE
Plasmid#168106PurposePiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloningDepositorInsertmCherry-KASH
UseCRISPR and Cre/LoxPromoterCAGAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST FNIP2
Plasmid#164982PurposeFNIP2 expressing vector for mammalian cellsDepositorAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph15-SNAPf-CCR5TC
Plasmid#135536PurposeEncodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
TagsSNAPfExpressionMammalianPromoterCAGAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP1-Myc
Plasmid#168503PurposeExpression of full length SRGAP1 with a C-terminal Myc fusionDepositorAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG GST DEPDC5
Plasmid#164983PurposeDEPDC5 expressing vector for mammalian cellsDepositorAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TWIN-STREP-FLAG-NPRL3
Plasmid#164984PurposeNPRL3 expressing vector for mammalian cellsDepositorAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP1076-Ai213 (TICL-EGFP-ICF-mOrange2-ICN-mKate2)
Plasmid#163491PurposeConditional expression of 3 different fluorophoresDepositorInsertEGFP, mOrange2, mKate2
UseMouse TargetingPromoterCAGAvailable SinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-G
Plasmid#164162PurposeExpresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminalDepositorInsertmCherry2
ExpressionMammalianPromoterCAGAvailable SinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-i53
Plasmid#119002PurposeMammalian expression of i53 (53BP1) inhibitor and BFPDepositorInserti53
ExpressionMammalianPromoterCAGAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBigTB-FlpERT2
Plasmid#149435Purposetamoxifen-inducible Flp recombinaseDepositorInsertFlp-ERT2 fusion protein
UseCre/Lox and Mouse TargetingTagsERT2ExpressionMammalianPromoterCAG promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hTAZ-FL
Plasmid#139979PurposeExpresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITRDepositorAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4580NES
Plasmid#138376PurposeBooster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.DepositorInsertBooster-JNK
ExpressionMammalianPromoterCAGAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1α-LNS2
Plasmid#128106PurposeExpress HA tagged Nrxn1α-LNS2DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW_2paRAF
Plasmid#129654PurposeFor generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1DepositorInsert2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
ExpressionMammalianPromoterCAGAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
Plasmid#114111Purposefusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
ExpressionMammalianPromoterCAGAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-dre
Plasmid#124832PurposeMonomeric TagBFP2 and dre recombinaseDepositorInsertmTagBFP2-T2A-dre
ExpressionMammalianPromoterCAGAvailable SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-V12
Plasmid#125136PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).DepositorInsertmNeon/mScarletP2A Rac1-V12
ExpressionMammalianMutationRemoved Not1 site in mScarlet. 12th AA in Rac1 ch…PromoterCAGAvailable SinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
BE3(E63Q)-P2A-EGFP (pRZ189)
Plasmid#123613PurposeCAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).DepositorInsertBE3(E63Q)-P2A-EGFP
ExpressionMammalianMutationE63Q in rAPOBEC1, D10A in SpCas9PromoterCAGAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector
Plasmid#114428PurposeTarget a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locusDepositorInsertGCaMP6f
UseCre/Lox and Mouse TargetingPromoterTRE2, CAGAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector
Plasmid#114429PurposeTarget a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette to the mouse TIGRE locusDepositorInsertGFP
UseCre/Lox and Mouse TargetingPromoterTRE2, CAGAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-Snapin
Plasmid#118743PurposeExpresses EGFP tagged SnapinDepositorAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Jaws-KGC-GFP-ER2
Plasmid#99233PurposeAAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter. Using bGH pA signal.DepositorInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterCAGAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)
Plasmid#104569PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domainsDepositorInsertdLbCpf1(D832A)-DmrA(x2)
Tags3x FLAG, 3x HA, and NLSExpressionMammalianMutationD832APromoterCAGAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)
Plasmid#104568PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domainDepositorInsertdLbCpf1(D832A)-DmrA(x1)
Tags3x FLAG, 3x HA, and NLSExpressionMammalianMutationD832APromoterCAGAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V
Plasmid#106190PurposeMedium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184V
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184VPromoterCAGAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA2
Plasmid#73982PurposeOtx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.DepositorInsertEGFP-Otx2-shRNA2
UseRNAiExpressionMammalianPromoterCAGAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAGhCBFA2T2
Plasmid#84363PurposeFLAG-HA tagged human CBFA2T2DepositorAvailable SinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Klf4-pA-pgk-hph
Plasmid#74906PurposeMammalian expression of mKlf4DepositorAvailable SinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only