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Showing: 1701 - 1750 of 13473 results

  3. p415-GalL-Cas9-CYC1t

    Plasmid
    #43804
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GalL
    Available since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43804-plasmid-map-sequence-id-59154

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  11. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Promoter
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100936-plasmid-map-sequence-id-206124

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  12. pScRad52-SpCas9 (pXK-1646)

    Plasmid
    #208823
    Purpose
    CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Use
    CRISPR; Cas9
    Tags
    Rad52
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    208823-plasmid-map-sequence-id-417236

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  17. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid
    #192663
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192663-plasmid-map-sequence-id-379915

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  18. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  20. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47948-plasmid-map-sequence-id-68812

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  25. PX380 Treponema denticola Cas9

    Plasmid
    #68317
    Purpose
    Treponema denticola Cas9
    Depositor
    Feng Zhang
    Insert
    TdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68317-plasmid-map-sequence-id-119529

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  26. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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  31. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

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  32. A3Ai-Cas9n-UGI-NLS

    Plasmid
    #109425
    Purpose
    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109425-plasmid-map-sequence-id-212947

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  33. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    51240-plasmid-map-sequence-id-410075

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  36. PX404 Campylobacter jejuni Cas9

    Plasmid
    #68338
    Purpose
    Campylobacter jejuni Cas9
    Depositor
    Feng Zhang
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    HA-NLS and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68338-plasmid-map-sequence-id-119561

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  37. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  44. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Tags
    Expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

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  50. pS34.EF1α<GFP-2A-Cas9-RC

    Plasmid
    #207404
    Purpose
    Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]
    Depositor
    Alexandros Poulopoulos
    Article
    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10
    Insert
    Cas9-RC
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP-2A
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    207404-plasmid-map-sequence-id-413204

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Showing: 1701 - 1750 of 13473 results