Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 8,860 results for: BLI;

Showing: 1751 - 1800 of 8860 results

  1. mOrange-pBAD

    Plasmid
    #54751
    Purpose
    Localization: Protein Expression Vector , Excitation: 548, Emission: 562
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6xHis and mOrange
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    54751-plasmid-map-sequence-id-453673

    Enlarge

  2. RedTrackCMV

    Plasmid
    #50957
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP
    Depositor
    James Bamburg
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    50957-plasmid-map-sequence-id-76710

    Enlarge

  3. pcDNA3 NT3

    Plasmid
    #39981
    Depositor
    Yves-Alain Barde
    Insert
    Neurotrophin 3 (Ntf3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  5. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid
    #196724
    Purpose
    Sortable and selectable EnAsCas12a.
    Depositor
    Bernhard Schmierer
    Insert
    enASCas12a-HA-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS - HA and SV40 NLS
    Expression
    Mutation
    E174R, S542R, K548R
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196724-plasmid-map-sequence-id-388065

    Enlarge

  6. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

    Plasmid
    #69100
    Purpose
    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP
    Depositor
    David Botstein
    Inserts
    yZ3EV cassette
    Z3 promoter
    yEGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69100-plasmid-map-sequence-id-122356

    Enlarge

  7. pBAD-FimH-9xHis

    Plasmid
    #97305
    Purpose
    E.coli UTI89 FimH signal peptide and lectin domain
    Depositor
    Per Svenningsen
    Insert
    Type 1 fimbiral adhesin FimH
    Use
    Tags
    9xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    97305-plasmid-map-sequence-id-182575

    Enlarge

  8. pMSCVhyg-DEST

    Plasmid
    #119747
    Purpose
    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Hygromycin resistance)
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119747-plasmid-map-sequence-id-233679

    Enlarge

  9. pET22B_SA_PcrA

    Plasmid
    #102999
    Purpose
    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
    Depositor
    Martin Webb
    Insert
    SA_PcrA
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    PcrA 199-2423
    Promoter
    T7
    Available since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102999-plasmid-map-sequence-id-199646

    Enlarge

  10. FUGW-H2B-mCherry

    Plasmid
    #132333
    Purpose
    Third generation lentiviral vector for hUbC-driven H2B-mCherry expression
    Depositor
    Bjoern Schwer
    Insert
    H2B-mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hUbC
    Available since
    July 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    132333-plasmid-map-sequence-id-258771

    Enlarge

  11. pLM433

    Plasmid
    #217293
    Purpose
    For creating CyanoTag vectors by Golden Gate cloning (BspQI version)
    Depositor
    Luke Mackinder
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mNeonGreen - 3x FLAG
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217293-plasmid-map-sequence-id-435614

    Enlarge

  12. pGBScomp High

    Plasmid
    #223202
    Purpose
    Group B Streptococcus High Expression Modular Complementation Vector
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    Complementation vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223202-plasmid-map-sequence-id-451880

    Enlarge

  13. pGBScomp Low

    Plasmid
    #223203
    Purpose
    Group B Streptococcus Low Expression Modular Complementation Vector
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    Complementation vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223203-plasmid-map-sequence-id-451879

    Enlarge

  14. ECFP-Peroxisomes-0

    Plasmid
    #54548
    Purpose
    Localization: Peroxisomes, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ECFP and Peroximal Targeting Signal 1 (PTS1)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pColdII-SIRPT1-Eptesicus fuscus

    Plasmid
    #78113
    Purpose
    Nt His-tag Eptesicus fuscus Sacsin 89-1368
    Depositor
    Kalle Gehring
    Insert
    Sacsin Eptesicus fuscus 89-1368
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78113-plasmid-map-sequence-id-145659

    Enlarge

  16. lentiGuide_puro-P2A-mNeonGreen

    Plasmid
    #221464
    Purpose
    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker
    Depositor
    James Eshleman
    Insert
    mNeonGreen
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6, EF-1a
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    221464-plasmid-map-sequence-id-438191

    Enlarge

  17. TLCV2-LoxP

    Plasmid
    #127098
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system.
    Depositor
    Adam Karpf
    Insert
    Cas9-2A-eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127098-plasmid-map-sequence-id-246495

    Enlarge

  18. pHAGE2-EF1aL-dsRed-W

    Plasmid
    #195863
    Purpose
    lentiviral expression of the red fluorescent protein driven by human EF1aL promoter
    Depositor
    Darrell Kotton
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    dsRed
    Expression
    Mutation
    Promoter
    EF1aL
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195863-plasmid-map-sequence-id-383935

    Enlarge

  19. p(+)MV-NSE-FlagP-F497A-3p

    Plasmid
    #58796
    Purpose
    Expresses recombinant measles virus with Flag-P-F497A gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag
    Expression
    Mutation
    MeV-P F497A
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58796-plasmid-map-sequence-id-91091

    Enlarge

  20. pExp-Sumo

    Plasmid
    #112574
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112574-plasmid-map-sequence-id-217243

    Enlarge

  21. pLys57-2

    Plasmid
    #211565
    Purpose
    Amber Suppressor tRNA for Lysine (Methionine-derived)
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211565-plasmid-map-sequence-id-421056

    Enlarge

  22. pLys57-3

    Plasmid
    #211566
    Purpose
    Amber Suppressor tRNA for Lysine (Isoleucine-derived)
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211566-plasmid-map-sequence-id-421057

    Enlarge

  23. pLys57-4

    Plasmid
    #211567
    Purpose
    Amber Suppressor tRNA for Lysine (Aspartate-derived)
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211567-plasmid-map-sequence-id-421059

    Enlarge

  24. PS-CFP2-Lifeact-7

    Plasmid
    #54695
    Purpose
    Localization: Actin, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    PS-CFP2-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pET-53-RtcA

    Plasmid
    #107876
    Purpose
    Expresses E. coli RtcA in bacterial expression cells
    Depositor
    Jay Hesselberth
    Insert
    E. coli RtcA (rtcA Escherichia coli)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    107876-plasmid-map-sequence-id-361816

    Enlarge

  26. pCDH-CMV-Nluc

    Plasmid
    #73038
    Purpose
    Express Nluc reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73038-plasmid-map-sequence-id-136036

    Enlarge

  27. pCAGG-NFIC2

    Plasmid
    #112701
    Purpose
    Expression of NFIC2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I C2 (Nfic Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112701-plasmid-map-sequence-id-212389

    Enlarge

  28. pTRIPZ (M)-HT-NLS

    Plasmid
    #82518
    Purpose
    Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Nuclear localizaition sequence
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82518-plasmid-map-sequence-id-156794

    Enlarge

  29. mCherry-KLC2-N-18

    Plasmid
    #62747
    Purpose
    Localization: Kinesin Light Chain , N1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    KLC2
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. pCMVlac-dirTopo-AU1-aGFP

    Plasmid
    #61971
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61971-plasmid-map-sequence-id-101286

    Enlarge

  31. pUC-dirTopo-P2A-aGFP

    Plasmid
    #61974
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61974-plasmid-map-sequence-id-101282

    Enlarge

  32. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61973-plasmid-map-sequence-id-101284

    Enlarge

  33. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

    Plasmid
    #191390
    Purpose
    Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(1-100)Cy-
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191390-plasmid-map-sequence-id-378098

    Enlarge

  34. pH6-TEV-mCherry

    Plasmid
    #132430
    Purpose
    Expresses His6-tagged mCherry in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    mCherry
    Use
    Tags
    N-terminal His6 tag with TEV protease site
    Expression
    Bacterial
    Mutation
    Codon optimized
    Promoter
    T7
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132430-plasmid-map-sequence-id-258769

    Enlarge

  35. pBHM2651

    Plasmid
    #229993
    Purpose
    Carries AID* tag, empty sgRNA, and NEOR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NEOR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229993-plasmid-map-sequence-id-461132

    Enlarge

  36. pCDH-CB-copGFP-T2A-iCre

    Plasmid
    #72256
    Purpose
    Express copGFP and iCre under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-iCre
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72256-plasmid-map-sequence-id-134470

    Enlarge

  37. pISV-DsRed1

    Plasmid
    #171024
    Purpose
    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus.
    Depositor
    Christian Staehelin
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2X EN 35S
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171024-plasmid-map-sequence-id-337720

    Enlarge

  38. mApple-Peroxisomes-2

    Plasmid
    #54627
    Purpose
    Localization: Peroxisomes, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Peroximal Targeting Signal 1 (PTS1) and mApple
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pY116 (pcDNA3.1-huMb2Cpf1)

    Plasmid
    #92292
    Purpose
    Expression of humanized Mb2Cpf1
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92292-plasmid-map-sequence-id-181491

    Enlarge

  40. pProk2.Cre.T2A.mCherry

    Plasmid
    #169013
    Purpose
    Expresses Cre under Prok2 minimal promoter in mammalian cells
    Depositor
    Michael Hastings
    Insert
    Cre Recombinase
    Use
    AAV and Cre/Lox
    Tags
    T2A and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Prokineticin 2
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169013-plasmid-map-sequence-id-337177

    Enlarge

  41. pET-His6-Sumo-CfaC-MBP

    Plasmid
    #126018
    Purpose
    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)
    Depositor
    Thomas Muir
    Insert
    His6-Sumo-CfaC-MBP
    Use
    Tags
    6xHis-SUMO and HA-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126018-plasmid-map-sequence-id-248773

    Enlarge

  42. dKeima-Red-N1

    Plasmid
    #54618
    Purpose
    Localization: N1 Cloning Vector, Excitation: 440, Emission: 616
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mKeima-Red
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. pCAGG-NFIB2

    Plasmid
    #112700
    Purpose
    Expression of NFIB2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112700-plasmid-map-sequence-id-212390

    Enlarge

  44. HA-IST1

    Plasmid
    #131619
    Purpose
    Expresses HA-tagged human IST1 in mammalian cells
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human IST1
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131619-plasmid-map-sequence-id-258201

    Enlarge

  46. pFBOH-MHL

    Plasmid
    #62304
    Purpose
    It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    6xHis and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62304-plasmid-map-sequence-id-102204

    Enlarge

  47. pBP-P_cymR-PCymRC

    Plasmid
    #204071
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    cymR repressor and PCymRC cumic acid-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204071-plasmid-map-sequence-id-413887

    Enlarge

  48. RHPMP138 (pLifeAct PA-mCH)

    Plasmid
    #88838
    Purpose
    Localization by Super-Resolution microscopy of lifeAct fused to PA-mCherry
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Use
    Tags
    PA-mCH
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88838-plasmid-map-sequence-id-177509

    Enlarge

  49. pCFP Paxillin

    Plasmid
    #50510
    Depositor
    Kenneth Yamada
    Insert
    Paxillin (PXN Human)
    Use
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. RHPMP139 (pLifeAct mEOS3.1)

    Plasmid
    #88839
    Purpose
    Localization by Super-Resolution microscopy of lifeAct fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Use
    Tags
    mEOS3.1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88839-plasmid-map-sequence-id-177489

    Enlarge

Showing: 1751 - 1800 of 8860 results