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Showing: 1751 - 1800 of 9150 results

  1. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  2. pExp-PpiB

    Plasmid
    #112572
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-PpiB and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112572-plasmid-map-sequence-id-217222

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  4. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  5. pET mRuby2 LIC cloning vector (H6-mRuby2)

    Plasmid
    #86934
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mR…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86934-plasmid-map-sequence-id-174750

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  7. pExp-DsbA

    Plasmid
    #112562
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbA(AA20-208) and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112562-plasmid-map-sequence-id-217223

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  8. pExp-DsbA(SS)

    Plasmid
    #112563
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbA(SS) and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112563-plasmid-map-sequence-id-217221

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  9. pCas9CR4-VQR

    Plasmid
    #120224
    Purpose
    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
    Depositor
    Christopher Reisch
    Insert
    SpCas9-VQR
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations D1135V/R1335V/T1337R
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120224-plasmid-map-sequence-id-233296

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  10. pET mClover LIC cloning vector (H6-mClover)

    Plasmid
    #86931
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mC…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86931-plasmid-map-sequence-id-174751

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  11. pExp-IF2

    Plasmid
    #112567
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-IF2 and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112567-plasmid-map-sequence-id-217215

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  12. pLS1/-11

    Plasmid
    #31492
    Depositor
    Kathleen Matthews
    Insert
    Lactose repressor with 11 amino acid C-terminal deletion (lacI E. coli variant)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  13. pet28a+_Yjjg

    Plasmid
    #220228
    Purpose
    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220228-plasmid-map-sequence-id-439539

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  14. pet15b _yein

    Plasmid
    #220226
    Purpose
    Bacterial expression of YeiN in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    psug (psuG E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220226-plasmid-map-sequence-id-436829

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  15. FtsZ-MEEVF

    Plasmid
    #74126
    Purpose
    Expresses FtsZ fused to the MEEVF peptide tag via a flexible linker
    Depositor
    Lynne Regan
    Insert
    FtsZ-MEEVF
    Use
    Tags
    C-terminal linker sequence with the MEEVF tag (se…
    Expression
    Bacterial
    Mutation
    Promoter
    T5-lac
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Use
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  22. pET MBP mClover LIC cloning vector (MBP-mClover)

    Plasmid
    #86930
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86930-plasmid-map-sequence-id-174752

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  23. pHL 636

    Plasmid
    #53015
    Purpose
    AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Inserts
    fimE
    fimB
    Use
    Synthetic Biology
    Tags
    Asp terminator, RBS(st7), and T1 terminator
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    53015-plasmid-map-sequence-id-116679

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  26. pET MBP tdTomato LIC cloning vector (MBP-tdTomato)

    Plasmid
    #86927
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86927-plasmid-map-sequence-id-181702

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  29. pVP16-tev-PRT1

    Plasmid
    #83405
    Purpose
    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83405-plasmid-map-sequence-id-175081

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  39. pKP2032

    Plasmid
    #167651
    Purpose
    Express His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
    Depositor
    Kathleen Postle
    Insert
    his6-exbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S1…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167651-plasmid-map-sequence-id-337296

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  44. pKP372

    Plasmid
    #167593
    Purpose
    Express TonB frameshift of amphipathic helix
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB fs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with frameshift of amphipathic helix
    Promoter
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    167593-plasmid-map-sequence-id-333696

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  45. PatoL-OG241

    Plasmid
    #72841
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoL
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoL
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72841-plasmid-map-sequence-id-133605

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  47. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  50. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive native promoters
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65006-plasmid-map-sequence-id-109903

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Showing: 1751 - 1800 of 9150 results