Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,586 results for: Coli

Clear keywords
Showing: 1751 - 1800 of 9586 results

  1. pSpec5'

    Plasmid
    #228549
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228549-plasmid-map-sequence-id-454858

    Enlarge

  2. pSpec3'

    Plasmid
    #228548
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228548-plasmid-map-sequence-id-454857

    Enlarge

  3. INT_Cargo_T7_gutB

    Plasmid
    #212139
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    xylitol dehydrogenase
    Expression
    Bacterial
    Promoter
    PT7
    Available Since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212139-plasmid-map-sequence-id-424777

    Enlarge

  4. pCT03

    Plasmid
    #212150
    Purpose
    Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.
    Depositor
    Kang Zhou
    Insert
    alanine dehydrogenase
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    PT7
    Available Since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212150-plasmid-map-sequence-id-420600

    Enlarge

  5. pExp-TFSumo

    Plasmid
    #112576
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TFSumo and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    112576-plasmid-map-sequence-id-255698

    Enlarge

  6. pExp-Tsf

    Plasmid
    #112578
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-Tsf and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112578-plasmid-map-sequence-id-217230

    Enlarge

  9. pTW5126

    Plasmid
    #129732
    Purpose
    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA-DUF::His8
    Tags
    9His tag
    Expression
    Bacterial
    Mutation
    replaces the domain of unknown function correspon…
    Promoter
    araBAD
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    129732-plasmid-map-sequence-id-257612

    Enlarge

  13. pTrc-trGPPS(CO)-LS

    Plasmid
    #50603
    Purpose
    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase
    Depositor
    Jay Keasling
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Inserts
    Limonene Synthase
    ApR
    LacIq
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    truncated codon optimized geranylpyrophosphate sy…
    Promoter
    Trc
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    50603-plasmid-map-sequence-id-77114

    Enlarge

  22. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

    Enlarge

  24. pHL 1158

    Plasmid
    #53007
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon and …
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pJBEI-6411

    Plasmid
    #47050
    Purpose
    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Promoter
    Pbad
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47050-plasmid-map-sequence-id-67072

    Enlarge

  33. pCRISPReporter

    Plasmid
    #65007
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    none
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65007-plasmid-map-sequence-id-109898

    Enlarge

  34. pCDF-NtXNtS1R1AtR2NtB2

    Plasmid
    #160881
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Expression
    Bacterial
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160881-plasmid-map-sequence-id-318841

    Enlarge

  38. pCDF-NtXNtS1R1AtR2B2

    Plasmid
    #160880
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Expression
    Bacterial
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160880-plasmid-map-sequence-id-318839

    Enlarge

  40. pCDF-NtXR1AtR2B2NtS1

    Plasmid
    #160879
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Expression
    Bacterial
    Promoter
    rbcS-S1: pBAD, chaperones: T7
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160879-plasmid-map-sequence-id-318813

    Enlarge

  41. pTE1059

    Plasmid
    #186628
    Purpose
    Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.
    Depositor
    Eriko Takano
    Article
    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10
    Inserts
    scbA
    scbB
    scbC
    Use
    Synthetic Biology
    Tags
    6xHis and 6xHis-thrombin
    Expression
    Bacterial
    Promoter
    Synthetic promoter B10, Synthetic promoter B19, a…
    Available Since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    186628-plasmid-map-sequence-id-371218

    Enlarge

Showing: 1751 - 1800 of 9586 results