161,152 results

1. pET151-GyrB

   Plasmid

   #216233

   Purpose

   S. aureus USA300 GyrB Codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-Pol-IIΔ

   Plasmid

   #35176

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of the entire C-terminal domain (repeats…

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-85b

   Plasmid

   #53282

   Purpose

   Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   crtE, crtY, crtI
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19/mChe-C

   Plasmid

   #183160

   Purpose

   Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAdEasy-2

   Plasmid

   #16401

   Purpose

   Adenoviral vector for use in AdEasy System, for use with large inserts.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. p16sheLL

   Plasmid

   #37320

   Depositor

   John Schiller

   Article

   Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

   Insert

   HPV16L1+L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CMV.ffLuciferase.SV40

   Plasmid

   #105532

   Purpose

   AAV expression of firefly luciferase from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   Luciferase
   

   Use

   AAV and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGDP1 NDM-1

   Plasmid

   #112883

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   NDM-1 (blaNDM-1 Klebsiella pneumoniae)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TREtight-mTagBFP2-B19G

   Plasmid

   #100799

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-ymTq2-URA3

    Plasmid

    #168057

    Purpose

    Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    ymTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1-Cdc42BPB

    Plasmid

    #50759

    Purpose

    expression of EGFP tagged Cdc42BPB

    Depositor

    Naoki Mochizuki

    Article

    Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

    Insert

    Cdc42BPB (CDC42BPB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBIS

    Plasmid

    #17817

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF3-microID

    Plasmid

    #172880

    Purpose

    expresses microID in mammalian cells with the tet-on or tet-off system

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    microID
    

    Use

    Luciferase and Retroviral; To be used in a tet-on…

    Tags

    myc

    Expression

    Mammalian

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    TRE-cmv

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-mCh-FYVE

    Plasmid

    #192289

    Purpose

    Expresses GST- and mCherry-tagged HRS FYVE in e Coli

    Depositor

    James Hurley

    Article

    Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

    Insert

    HRS FYVE domain
    

    Use

    Tags

    GST-TEV-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-tagged Apollo-NADP+

    Plasmid

    #71797

    Purpose

    Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

    Depositor

    Jonathan Rocheleau

    Article

    Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

    Insert

    Apollo-NADP+
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. psiCheck-ApoECDS

    Plasmid

    #41848

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    ApoE CDS (APOE Human)
    

    Use

    Luciferase

    Tags

    renilla luciferase

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pLNC Wnt-5bHA

    Plasmid

    #18034

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5b (Wnt5b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-EWB-DIO-cyan pre-eGRASP(p32)

    Plasmid

    #111589

    Purpose

    An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    cyan pre-eGRASP(p32)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pH6HTC_BRD2-Halo

    Plasmid

    #175336

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    BRD2 (BRD2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CMV-mCherry-NLS

    Plasmid

    #165442

    Purpose

    Expresses nuclear mCherry from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    mCherry-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-EGFP-Rac1(wt)

    Plasmid

    #13719

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    EGFP-Rac1(wt) (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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24. R713-M91-303: CMV51p> Halotag-Hs.NRAS

    Plasmid

    #159682

    Purpose

    Mammalian protein expression of NRAS-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.NRAS (NRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Rb-379-928-pGex2TK

    Plasmid

    #21749

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928 of wt Rb …

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. Str-KDEL_CD63-SBP-mCherry

    Plasmid

    #222326

    Purpose

    Synchronize the trafficking of CD63 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Flag-HA-USP15

    Plasmid

    #22570

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP15 (USP15 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCDNA3.1-pCMV-PfV-GS-Sapphire

    Plasmid

    #116933

    Purpose

    Mammalian vector for expression of the 40nm GEM

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Tags

    Sapphire

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1

    Plasmid

    #118169

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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