165,343 results

1. p2R3a-GUS-OcsT

   Plasmid

   #71267

   Purpose

   Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Beta-glucuronidase
   

   Use

   Gateway

   Tags

   2xGly linker and octaline synthase terminator

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE-Tn5-161

   Plasmid

   #118541

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEM047 [T7-assisted CROPseq sgRNA expression vector]

   Plasmid

   #224899

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

   Depositor

   Thomas Norman

   Article

   Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-GR

   Plasmid

   #215094

   Purpose

   Expresses HA-tagged Glucocorticoid Receptor in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   NR3C1 (NR3C1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-flag YAP2 5SA

   Plasmid

   #27371

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP2 (YAP1 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Serines at positions 61, 109, 127, 128, 131, 163,…

   Available Since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. hsDCX-EGFP-pHAT

   Plasmid

   #83918

   Purpose

   human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

   Depositor

   Gary Brouhard

   Article

   Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

   Insert

   Doublecortin isoform b (DCX NM_178152.2, Human)
   

   Tags

   6-His and EGFP+Strep-tag II

   Expression

   Bacterial

   Available Since

   Dec. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 myc-RNF114

   Plasmid

   #58295

   Purpose

   mammalian expression of myc tagged RNF114

   Depositor

   Francesca Capon

   Article

   Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

   Insert

   RNF114 (RNF114 Human)
   

   Tags

   his and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-eGFP-pA

   Plasmid

   #107281

   Purpose

   donor vector backbone with constitutive eGFP

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   GFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPI356

   Plasmid

   #207449

   Purpose

   Extrachromosomal expression vector PkgE-PH-GFP and PTEN-mCherry

   Depositor

   Rob Kay

   Article

   Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

   Inserts

   PkgE
   PTEN
   

   Use

   Unspecified

   Tags

   GFP and mCherry

   Mutation

   PH domain only

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDM1383

    Plasmid

    #207423

    Purpose

    Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

    Depositor

    Rob Kay

    Article

    Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

    Inserts

    HSPC300
    Raf1 (RAF1 Human)
    

    Use

    Unspecified

    Tags

    GFP and mCherry

    Mutation

    aa 1–134 (RBD) only

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. XX01CAPN1A-c001

    Plasmid

    #175491

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    CAPN1 / CAPNS1 (CAPN1 Human)
    

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-ADRBK1

    Plasmid

    #23835

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ADRBK1 (GRK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJFRC150-20XUAS-IVS-Flp1::PEST

    Plasmid

    #32132

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    Flippase1::PEST
    

    Tags

    PEST

    Expression

    Insect

    Mutation

    Aspartic Acid at Amino Acid 5

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTHSSe_46

    Plasmid

    #109244

    Purpose

    pSC101 var4 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF1-Hs Cdk1/cyclin B1

    Plasmid

    #177330

    Purpose

    Co-expression of the human active Cdk1/cyclin B1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-ApplePy1Highest

    Plasmid

    #239064

    Purpose

    Mammalian expression of highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    ApplePy1Highest
    

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-X1-Neo-GFP-ATL2

    Plasmid

    #109020

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2 (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    EIF-1a promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSF-G1mCNPRS

    Plasmid

    #174718

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn-eGFP-2A-mCherry_D4H

    Plasmid

    #194875

    Purpose

    Ratiometric D4H sensor for in vivo neuron-specific cholesterol visualization

    Depositor

    Peter Scheiffele

    Article

    Czernecki et al bioRxiv 2022.11.16.516849

    Insert

    GFP-T2A-mCherry-D4H
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAG/HA-hBIG2

    Plasmid

    #226254

    Purpose

    Expresses N-terminally HA-tagged hBIG2 in mammalian cells

    Depositor

    Hye-Won Shin

    Insert

    BIG2 (ARFGEF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLenti PGK GFP Puro (w509-5)

    Plasmid

    #19070

    Purpose

    3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCI-neo beta catenin delta45

    Plasmid

    #16520

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    beta catenin (CTNNB1 Human)
    

    Expression

    Mammalian

    Mutation

    Delta 45 (HCT116 mutation)

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

    Plasmid

    #204579

    Purpose

    lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    mCherry
    

    Use

    Lentiviral

    Promoter

    TRE3G

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pMRE-Tn5-160

    Plasmid

    #118540

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSL3718 (pQCascade_Pse)

    Plasmid

    #200899

    Purpose

    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCas7, PseCas8, PseCas6, PseTniQ
    

    Tags

    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. p20StAD

    Plasmid

    #190268

    Purpose

    Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

    Depositor

    Assaf Mosquna

    Article

    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast genome-integrating vector

    Tags

    GAL4-AD

    Promoter

    TDH3

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBbE8k-RFP

    Plasmid

    #35270

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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