We narrowed to 7,452 results for: Lif;
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Plasmid#188944PurposeMiddle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherryDepositorInsertmito-APEX2_p2A_APEX2-H2B_p2A_mCherry
UseMiddle entry vector for gateway (l1-l2)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC4
Plasmid#183199PurposeEntry cloneDepositorInsertCas9-RFC4
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA)
Plasmid#123150PurposeMercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJH2827
Plasmid#179542PurposePnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPnmr-1Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2d10-364delta263-295-TEV-STREP
Plasmid#171830PurposeMammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutation10-364delta263-295PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K395A/K396A
Plasmid#162656PurposeExpresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K395 and K396 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJP72
Plasmid#176479PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoterDepositorInsertsactin > GenR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B L69E
Plasmid#175026PurposeExpresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (L69E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationLeucine 69 to Glutamate (CTA to GAA)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH3409
Plasmid#179574PurposePceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPceh-12Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'UAG)
Plasmid#170128PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin4a-Clover
Plasmid#163853PurposeZebrafish muscle specific expression of Cavin4a-Clover, Parton lab clone DPVDepositorInsertCavin4a (cavin4a Zebrafish)
UseZebrafish expressionTagsCloverExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS84(RS)
Plasmid#123155PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS84, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet
Plasmid#83286Purposeuntargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pairDepositorInsertmTurquoise2-FHA2-AURKB substrate-YPet
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SAC1-HLx8
Plasmid#108139PurposeSAC1 with extended linkerDepositorInsertmCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM01
Plasmid#135414PurposeExpression of Hygromycin resistance from CMV promoterDepositorInsertCMVpr hph ADH1t
UseTagsExpressionBacterial and YeastMutationPromoterCMVprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SMT205
Plasmid#134817PurposeSMT201 with mutated RBS for DHFR, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
Plasmid#130665PurposePlasmid to test the efficiency of T2A multiplexing in Aedes aegyptiDepositorInsertAaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
UseAe. aegypti expressionTagsExpressionInsectMutationPromoterAedes aegypti poly-ubiquitinAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-ArchT-GFP
Plasmid#86536PurposeRetrovirus expressing optogenetic silencer ArchT-GFPDepositorInsertArchT
UseRetroviralTagsGFPExpressionMammalianMutationPromoterSynapsin I (human)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-FLAG PLU-HIS
Plasmid#113173PurposeBaculovirus expression of PNG and PLUDepositorInsertPNG, PLU
UseBaculovirusTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH104
Plasmid#48262PurposeTemplate plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.DepositorInsertsTEF1p 5' fragment
URA3 5' fragment
TEF1p 3' fragment
UseRoutine cloning vectorTagsExpressionMutationFrom SK1 background. -343 to -1 relative to TEF1 …PromoterTEF1pAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330) S278C
Plasmid#87739PurposeBacterial expression of a VSL domain variant of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-277, mutated Ser 278 to CysPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-chimpTRIM5-FOS
Plasmid#79065PurposeExpress chimpanzee TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(chimpanzee)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(81-437)
Plasmid#87734PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-80PromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2_Rluc-Y0x-Fluc
Plasmid#233614PurposeExpression of Rluc-Fluc reporter gene containing no YDepositorInsertRluc-Y0x-Fluc
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-FlpTag-DsRed-HDR-ph2
Plasmid#231902PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-FlpTag-DsRed-HDR-ph1
Plasmid#231901PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-FlpTag-DsRed-HDR-ph0
Plasmid#231900PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEW107
Plasmid#232796PurposeCOMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2abDepositorUseTagsHis6-3xFLAG, TwinstrepExpressionInsectMutationySet1(762-1080)Promoterpolyhedrin promoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-1_Rluc-Y39x-Fluc
Plasmid#233615PurposeExpression of Rluc-Fluc reporter gene containing 39xYDepositorInsertRluc-Y39x-Fluc
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GEM
Plasmid#187648PurposeExpresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.DepositorInsertPfV
UseAAVTagsHaloTag and SNAP-tagExpressionMammalianMutationPromoterSynapsinAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Evectin-2
Plasmid#221754PurposeStable expression of Evectin-2 in mammalian cellsDepositorInsertPLEKHB2 (PLEKHB2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem PLEKHB2(PH domain) (amino acids 1-110)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PLCd1(PHD)
Plasmid#221753PurposeStable expression of GFP-PLCd1(PHD) in mammalian cellsDepositorInsertPLCd1 (PLCD1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–175PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-2xFYVE
Plasmid#221750PurposeStable expression of GFP-2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain
UseRetroviralTagsGFPExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-GAC site)
Plasmid#170149PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcriptDepositorInsertBoxB-RAB7A(GAC)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (RAB7A-GAC site)
Plasmid#170140PurposeAAV vector carrying a MS2 adRNA targeting the RAB7A transcriptDepositorInsertMS2-RAB7A(GAC)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1(W795R)-StayGold
Plasmid#210822PurposeMammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (W795R)DepositorInsertProminin 1 (PROM1 Human)
UseTagsStayGoldExpressionMammalianMutationChanged Trp-795 to ArgPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1-TwinStrep-His
Plasmid#210816PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)DepositorInsertProminin 1 (PROM1 Human)
UseTagsTwinStrep, 10xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1-StayGold
Plasmid#210821PurposeMammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)DepositorInsertProminin 1 (PROM1 Human)
UseTagsStayGoldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Halo-MDC1 PST deletion
Plasmid#207109PurposepHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the PST repeat region in human cells.DepositorInsertHaloTag-MDC1 PST deletion
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-MDC1 BRCT domain deletion
Plasmid#207110PurposepHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.DepositorInsertHaloTag-MDC1 BRCT domain deletion
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1161
Plasmid#217964PurposeFor RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFP, APEXExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1313
Plasmid#217955PurposeExpression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP
Plasmid#179717PurposeLentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFPDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
3H-α-synuclein
Plasmid#205714PurposeMammalian expression of 3H-alpha-synucleinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsthermostable three helix-bundle protein 3HExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
α-synuclein-3H
Plasmid#205715PurposeMammalian expression of alpha-synuclein-3HDepositorInsertalpha-synuclein (SNCA Human)
UseTagsthermostable three helix-bundle protein 3HExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pETM11-6xHis-TEV-hORF1p_StammerAAA
Plasmid#202587PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…PromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only