162,981 results

1. pVQ CMV NanoV1-2a-EGFP ferritin

   Plasmid

   #79649

   Purpose

   Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

   Depositor

   Jeffrey Friedman

   Article

   Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

   Inserts

   transient receptor potential vanillin 1 (Trpv1 Rat)
   camelid anti-GFP nanobody
   Enhanced green fluorescent protein
   mouse ferritin light chain (Ftl1 Mouse)
   mouse ferritin heavy chain (Fth1 Mouse)
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18T-mini-Tn7T-Gm-eyfp

   Plasmid

   #65031

   Purpose

   GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   eyfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL4-ERSE1-luc2P-Hygro

   Plasmid

   #101789

   Purpose

   Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

   Depositor

   Seiichi Oyadomari

   Article

   Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

   Insert

   3xERSE-I
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   minimal TATA-box promoter with low basal activity

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2058

   Plasmid

   #141898

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-2 (NKX2-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-Tp-gfpmut3

   Plasmid

   #65033

   Purpose

   TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTA-Mob 2.0

   Plasmid

   #149662

   Purpose

   Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

   Depositor

   Bogumil Karas

   Article

   Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

   Type

   Empty backbone

   Use

   Synthetic Biology; Conjugation helper plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL-c2X_MBP-CLmN-H6

   Plasmid

   #234487

   Purpose

   Maltose binding protein N-terminally fused to His-tagged CLmN split intein

   Depositor

   Henning Mootz

   Article

   Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

   Insert

   N-terminal Fragment of the CLm Split Intein
   

   Use

   Tags

   Hexahistidine Tag and Maltose Binding Protein (MB…

   Expression

   Bacterial

   Mutation

   T69K, F75H, M118N within the N-terminal fragment …

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-FLAG-Rheb

   Plasmid

   #19996

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   Ras homolog enriched in brain 1 (RHEB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAJM.773

   Plasmid

   #108527

   Purpose

   VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   VanRAM + PVanCC-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV5-ActRIIB

    Plasmid

    #13501

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    Activin receptor IIB (Acvr2b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. GRB2-V5

    Plasmid

    #139631

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-G-Flamp1-mut

    Plasmid

    #188568

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-Rheb-N153T

    Plasmid

    #19997

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 - N153T (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Asparagine 153 to Threonine

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pOpen-Tthlig

    Plasmid

    #165569

    Purpose

    Taq DNA Ligase

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    Tth DNA Ligase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCasKP-hph

    Plasmid

    #117232

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1a-CCO-IRES-Puromycin

    Plasmid

    #134861

    Purpose

    Expresses EGFP-tagged inner mitochondrial membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    cytochrome c oxidase subunit VIII (COX8A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-GFP-NMHC II-B

    Plasmid

    #11348

    Depositor

    Robert Adelstein

    Article

    Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

    Insert

    NMHC II-B (MYH10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Dm-snRNA:U6:96Ab

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRE YIC

    Plasmid

    #18784

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Insert

    H-ferritin iron response element
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPAP004

    Plasmid

    #160911

    Purpose

    E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

    Insert

    lacZ selection cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge