19,755 results

1. pYES2-Ub-R-GFP

   Plasmid

   #11953

   Depositor

   Nico Dantuma

   Article

   Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

   Insert

   Ub-R-GFP
   

   Expression

   Yeast

   Mutation

   Ubiquitin fused to N-terminus of GFP. Arginine at…

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPER.PKCepsilon.RNAi

   Plasmid

   #10798

   Depositor

   Alex Toker

   Article

   Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

   Insert

   PKC epsilon RNAi (PRKCE Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   April 7, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pGP-CMV-GCaMP6s-CAAX

   Plasmid

   #52228

   Purpose

   Plasma membrane targeted GCaMP6s, calcium sensor

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   GCaMP6s-CAAX
   

   Tags

   6xHis and CAAX

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-Hylight

   Plasmid

   #193447

   Purpose

   A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

   Depositor

   Richard Goodman

   Article

   Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

   Insert

   Hylight Biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. STING-miRFP680

   Plasmid

   #200940

   Purpose

   STING fused with miRFP680 for live cell imaging

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   STING (STING1 Human)
   miRFP680
   

   Use

   Lentiviral

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #102796

   Purpose

   Retrovirus for delivery of one sgRNA (empty, bbsI)

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   U6 sgRNA
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-TRE-dCas9-KRAB-MeCP2-BSD

   Plasmid

   #140690

   Purpose

   Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   Cas9m4-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiMPH v2

   Plasmid

   #89308

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Insert

   MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB-cLUC

   Plasmid

   #174051

   Purpose

   Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

   Depositor

   Alberto Macho

   Article

   Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Plant

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mNeonGreen

    Plasmid

    #189976

    Purpose

    Part 3-4-5 plasmid to introduce mNeonGreen into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Cloning

    Promoter

    N/A

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral Gateway destination vector. Blasticidin selection, V5 tag.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. scAAV-hIBA1a-GFP

    Plasmid

    #214146

    Purpose

    Self-complementary AAV vector packaging GFP under the human Iba1 promoter

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Insert

    GFP
    

    Use

    AAV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mito-gTEMP_pcDNA3

    Plasmid

    #109117

    Purpose

    Mitochondria targetting gTEMP

    Depositor

    Takeharu Nagai

    Article

    Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

    Insert

    mito-gTEMP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZT-C13-R1

    Plasmid

    #62197

    Purpose

    Chr.13/CLYBL TALEN expression vector

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Right CLYBL TALEN
    

    Use

    TALEN

    Promoter

    CMV

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcw107

    Plasmid

    #62511

    Purpose

    Gateway Expression Vector

    Depositor

    John Doench, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TO-NFIA.1-SOX9

    Plasmid

    #182310

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP scFv [N86/38]

    Plasmid

    #182095

    Purpose

    Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting GFP
    

    Tags

    HA, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-U6-crRNA-empty

    Plasmid

    #134921

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    crRNA for CRISPR-Cas3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJMP1039

    Plasmid

    #119239

    Purpose

    expresses Tn7 transposase

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    tnsABCD
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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