166,041 results

1. pSBbi G418R mbIL21

   Plasmid

   #194329

   Purpose

   Consitiutive mammalian expression of membrane bound human IL-21

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   mbIL-21 (IL21 Human)
   

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Z-lock cofilin

   Plasmid

   #141137

   Purpose

   controlling the actin-severing ability of cofilin with light

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Expression

   Mammalian

   Mutation

   mCherry, Zdk2(132F), Cofilin S3A, LOV WT

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

   Plasmid

   #175529

   Purpose

   Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag7
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xAP1pGL3 (3xAP-1 in pGL3-basic)

   Plasmid

   #40342

   Depositor

   Alexander Dent

   Article

   Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

   Insert

   3xAP-1
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains three canonical AP-1 binding sites (TGAC…

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2/1

   Plasmid

   #112862

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap1
   

   Use

   AAV

   Promoter

   Truncated P5

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-HA-UCHL1

   Plasmid

   #22563

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   UCHL1 (UCHL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 Flag beta-1-adrenergic-receptor

   Plasmid

   #14698

   Depositor

   Robert Lefkowitz

   Article

   Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

   Insert

   B1AR (ADRB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAX APP C99

   Plasmid

   #30146

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   APP C99 (APP Human)
   

   Expression

   Mammalian

   Mutation

   C-terminal from BACE site of APP

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYES2-Ub-M-GFP

    Plasmid

    #11952

    Depositor

    Nico Dantuma

    Article

    Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

    Insert

    Ub-M-GFP
    

    Expression

    Yeast

    Mutation

    Ubiquitin fused to N-terminus of GFP. Methionine …

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. VPS13A^Halo

    Plasmid

    #118759

    Purpose

    VPS13A internally tagged with Halo at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Tags

    Halo tag inserted at position 1372

    Expression

    Mammalian

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHcRed-NPM1wt-C1

    Plasmid

    #131818

    Purpose

    Expresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB405

    Plasmid

    #74799

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJQ200SK

    Plasmid

    #78497

    Purpose

    Suicide vector for gene replacement

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide plasmid

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-hERG1a

    Plasmid

    #53052

    Purpose

    Expresses alpha subunit of hERG1a K channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAY5 (mAID-EGFP-NLS piggyBac)

    Plasmid

    #140532

    Purpose

    mAID-EGFP-NLS piggyBac

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC18-mini-Tn7T-Gm-lux

    Plasmid

    #64963

    Purpose

    mini-Tn7 luxCDABE transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    lux operon
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GST-HA-GFP11-N1

    Plasmid

    #91953

    Purpose

    Expresses GST-HA-GFP11 in mammalian cells

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    GST-HA-GFP11
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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