163,461 results

1. pTXTL-T7max-FLuc

   Plasmid

   #188311

   Purpose

   Expression of Firefly luciferase via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   Firefly luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7Max

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHM066

   Plasmid

   #225524

   Purpose

   Encodes Wnt-responsive destablized iRFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Insert

   iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GCH1_pLX307

   Plasmid

   #98336

   Purpose

   Lentiviral expression of GCH1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   GCH1 (GCH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

   Plasmid

   #202689

   Purpose

   CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

   Depositor

   Stanley Qi

   Article

   Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

   Inserts

   CopGFP
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ8079 - IL2RG GFP-P2A CLIP Donor

   Plasmid

   #202690

   Purpose

   CLIP donor template for integrating GFP into IL2RG locus.

   Depositor

   Stanley Qi

   Article

   Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

   Insert

   CopGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcw107

   Plasmid

   #62511

   Purpose

   Gateway Expression Vector

   Depositor

   John Doench, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. KW57_HART_RAC1

   Plasmid

   #207399

   Purpose

   Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

   Depositor

   Stephen Floor

   Article

   Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE2

   Plasmid

   #132775

   Purpose

   Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+HyPer7

   Plasmid

   #136466

   Purpose

   Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCBASceI

    Plasmid

    #26477

    Purpose

    I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

    Depositor

    Maria Jasin

    Article

    Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

    Insert

    pCBASceI
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-GCaMP6f

    Plasmid

    #182548

    Purpose

    Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

    Depositor

    Chris Richards

    Article

    Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

    Insert

    ER localized P450
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 - TRC control

    Plasmid

    #10879

    Purpose

    Negative control shRNA vector containing non-hairpin insert.

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV40-EF1a-hBCL10-SO

    Plasmid

    #75256

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs, EF1a

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInducer20

    Plasmid

    #44012

    Purpose

    Tet-inducible lentiviral vector for ORF expression

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Bxb1

    Plasmid

    #182142

    Purpose

    Expression of human codon-optimized Bxb1

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    human codon optimized Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MBP-Benzonase

    Plasmid

    #218192

    Purpose

    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

    Depositor

    Cheryl Arrowsmith

    Insert

    nucA
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. T444T

    Plasmid

    #113081

    Purpose

    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. paavCAG-iCre

    Plasmid

    #51904

    Purpose

    For AAV expression of iCre recombinase

    Depositor

    Jinhyun Kim

    Article

    Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

    Insert

    improved Cre recombinase
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

    Plasmid

    #44361

    Purpose

    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV; Adeno associated viral vector

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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