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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Publication

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Promoter

T7

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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CRE recombinase

Plasmid

#62730

Purpose

Expresses Cre recombinase protein in bacterial cells

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Cre recombinase

Use

Cre/Lox

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCR4-24XPP7SL

Plasmid

#31864

Depositor

Robert Singer

Publication

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

24xPP7 Stem Loop Cassette

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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Flag-HA-USP10

Plasmid

#22543

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP10 (USP10 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pminiTol2

Plasmid

#31829

Purpose

Vector with minimal Tol2 transposition sites, with capacity for delivering larger gene cargo than standard Tol2 vectors

Depositor

Stephen Ekker

Publication

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Type

Empty backbone

Use

Cloning vector

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pLenti CMV/TO Hygro DEST (693-2)

Plasmid

#17291

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pTight-9-124-BclxL

Plasmid

#60857

Purpose

2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

pTight

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pFLPo

Plasmid

#13792

Purpose

Expression of mouse codon-optimized FLP recombinase

Depositor

Philippe Soriano

Publication

Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

Insert

FLPo

Use

Cloning vector

Expression

Bacterial

Available since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCMV-HA-JMJD2A

Plasmid

#24180

Purpose

Mammalian expression of human JMJD2A with HA tag

Depositor

Kristian Helin

Publication

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2A (KDM4A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBOB-CAG-SARS-CoV2-Spike-HA

Plasmid

#141347

Purpose

3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

Depositor

Gerald Pao

Publication

Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

Insert

SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))

Use

Lentiviral

Tags

HA

Mutation

resynthesized with human codon optimization nucle…

Promoter

CAG

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Publication

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSRK2706

Plasmid

#78571

Purpose

Expresses untagged human rhinovirus 3C protease in E. coli

Depositor

David Waugh

Publication

Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

Insert

human rhinovirus 3C protease (HRVBgp1 Human)

Expression

Bacterial

Promoter

T7

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-C1 F-tractin-EGFP

Plasmid

#58473

Purpose

Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

Depositor

Dyche Mullins

Publication

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

F-tractin (Itpka Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Publication

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLenti6-DEST PINK1-V5 WT

Plasmid

#13320

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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c-myc-PT3EF1a

Plasmid

#92046

Purpose

Expresses c-Myc in mammalian cells

Depositor

Xin Chen

Publication

Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

Insert

C-MYC (MYC Human)

Expression

Mammalian

Promoter

EF-1a

Available since

July 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176269

Purpose

Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Publication

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCasMINI-V4-VPR-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSLIK-IDH1-R132H-FLAG

Plasmid

#66803

Purpose

Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

Depositor

Christian Metallo

Publication

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

R132H

Promoter

CMV

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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