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Plasmid#217005Purposelentiviral plasmid for gRNA cloning with selectable mCherry expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term
Plasmid#182925PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneUseTagsAPEX2 and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVM
Plasmid#64086PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain matrix geneDepositorInsertVSV matrix protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAVS-Pex
Plasmid#44557PurposeRetroviral vector for expression of a chimeric MAVS allele targeted to peroxisomes in mammalian cellsDepositorInsertMAVS-Pex13 chimeric protein (MAVS Human)
UseRetroviralTagsExpressionMammalianMutationcontains aa1-500 of MAVS and aa136-233 of Pex13PromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
iOn-CAG∞MCS
Plasmid#154013PurposeExpression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoterDepositorInsertMCS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPble
Plasmid#63561PurposeBleomycin drug selection markerDepositorInsertBleomycin drug selection marker
UseLeishmania expressionTagsExpressionMutationPromoterAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mScarlet
Plasmid#131001PurposeAAV vector to drive the expression of mScarlet under the control of human Synapsin promoterDepositorHas ServiceAAV1 and AAV8InsertmScarlet
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Oct3/4-IRES-Neo
Plasmid#13461DepositorInsertOct3/4 (Pou5f1 Mouse)
UseTagsExpressionMammalianMutationP31SPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)
Plasmid#149722PurposeLentiviral expression of crRNA with GFP and a neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Lamp2b-HA
Plasmid#71292PurposeFlag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFlag, HA, and Lamp2 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP680-N1
Plasmid#136557PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)DepositorInsertmiRFP680
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mScarlet-I
Plasmid#98816PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorArticleInsert3xNLS
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET23b-15F11-HA-mEGFP-6xHis
Plasmid#129593PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin
Plasmid#211471Purposelentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistanceDepositorInsertCas9_T2A_mNeonGreen_P2A_BlasticidinR
UseCRISPR and LentiviralTags3xFlagExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4max-NRRH
Plasmid#136918PurposeExpresses BE4max-NRRH in mammalian cellsDepositorInsertBE4max-NRRH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-2 sfGFP
Plasmid#154464PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 N-term GFP tTA
Plasmid#107551PurposeAll-in-one dox-repressible expression of cDNA with optional N-term GFP tag.DepositorTypeEmpty backboneUseLentiviralTagsGFP (optional)ExpressionMammalianMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -