We narrowed to 33,749 results for: IND

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Flag-MCPIP1(150-453)

Plasmid

#90109

Purpose

Mammalian expression of Flag-MCPIP1(150-453)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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MEIS1B-pSP65

Plasmid

#21019

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1B (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pEPQD1CB0119

Plasmid

#177051

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein+H34like (NmMLPL) from Nepeta mussinii driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

major latex protein-like (NmMLPL) from Nepeta mussinii

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGex-3x Flag MKK3

Plasmid

#14814

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK3 (MAP2K3 Human)

Tags

Flag

Expression

Bacterial

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pME-hygro-hPGAP3-3HA

Plasmid

#50373

Purpose

Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)

Tags

3x HA

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
BII-ChBtW-eSpCas9

Plasmid

#133370

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

Flag

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMP472

Plasmid

#134997

Purpose

Interspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

Depositor

Ahmad Khalil

Article

Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

Insert

Inters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

Use

AAV and Synthetic Biology

Expression

Mammalian

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMP498

Plasmid

#134998

Purpose

Clustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

Depositor

Ahmad Khalil

Article

Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

Insert

Clust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

Use

AAV and Synthetic Biology

Expression

Mammalian

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorT(sp)

Plasmid

#111255

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorT signal peptide

Expression

Bacterial

Promoter

Pc

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-N

Plasmid

#128308

Purpose

Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 N terminal fragment (OB fold) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (1–138 aa)

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hB50

Plasmid

#18967

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

Insert

Brevican N-terminus (BCAN Human)

Expression

Mammalian

Mutation

Human brevican N-terminus aa 1-400

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83,88,104A

Plasmid

#58405

Purpose

Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysines 83, 88, and 104 to Alanine

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83A

Plasmid

#58401

Purpose

Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24A

Plasmid

#58400

Purpose

Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 24 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pTK157_mCherry-4.1G-CTD

Plasmid

#46359

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G C-terminal domain (EPB41L2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

C-terminal domain from 886-1005 amino acids

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm4_A

Plasmid

#145883

Purpose

Insect Expression of DmLSm4

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm4 (LSm-4 Fly)

Tags

V5-6xHis

Expression

Insect

Mutation

two silent mutations compared to the sequence of …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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GST-TEVcs-GFP-ATG101

Plasmid

#171415

Purpose

For the purifcation of GFP-ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ATG101 (ATG101 Synthetic)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Ago3m

Plasmid

#21531

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago3 (AGO3 Human)

Tags

GFP

Expression

Mammalian

Mutation

missing aa757-823, the C-termianl 66aa of PIWI do…

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only
pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

Plasmid

#83286

Purpose

untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

mTurquoise2-FHA2-AURKB substrate-YPet

Use

Retroviral

Expression

Mammalian

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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PCBP4_3xKH_pGEX

Plasmid

#135156

Purpose

Recombinant expression of PCBP4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PCBP4 (PCBP4 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_C

Plasmid

#146086

Purpose

Mammalian Expression of HsTNRC6A

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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GST-GFP-ATG14

Plasmid

#171426

Purpose

For the purifcation of GFP- PI3KC3 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ATG14 (ATG14 Human)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-myc-Uba1

Plasmid

#29508

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Uba1 (UBA1 Budding Yeast)

Tags

His and myc

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-mTAZ-WW (86)

Plasmid

#40915

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains mTAZ 14-3-3 binding site and WW domain (…

Promoter

Tac

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

Plasmid

#69226

Purpose

Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJK526

Plasmid

#155387

Purpose

PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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lenti SYN-SVI-dCas9-VPR

Plasmid

#164575

Purpose

Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

Insert

FLAG-dCas9-VPR containing an SV40 intron

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pEnt R3L2 TetO(sh) mCh-Rs1-2

Plasmid

#24417

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Rs1

Use

Entry vector

Tags

FLAG and mCherry

Mutation

Human 5HT4B receptor with D100A mutation, generat…

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-PDZ(HtrA2)

Plasmid

#16207

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

Insert

HtrA2 (HTRA2 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 350–459

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only