-
Plasmid#112262PurposeExpress g-AAVS1DepositorInsertg-AAVS1
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDY1513 AAVS1 sgRNA
Plasmid#234832PurposesgRNA for AAVS1 STITCHR insertionDepositorInsertAAVS1 sgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 T2 CRIPR in pX330
Plasmid#72833PurposeCRISPR/Cas to target the AAVS1 locus in human cellsDepositorInsertCas9 and gRNA for targeting the AAVS1 locus in human cells
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-CAG-hrGFP
Plasmid#52344PurposeAAVS1 donor vector for constitutive gene expressionDepositorInserthrGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-DEST
Plasmid#80490PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 TALEN Left
Plasmid#52341PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Left
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 TALEN Right
Plasmid#52342PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Right
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Nst-MCS
Plasmid#80487Purposedonor vector for AAVS1 targeting (neomycin selection) with multiple cloning siteDepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PDi-CRISPRn
Plasmid#73500PurposeDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locusDepositorInsertsCas9
rtTA
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-EGFP
Plasmid#52343PurposeAAVS1 donor vector for inducible gene expressionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-sgAAVS1-HepmTurquoise2
Plasmid#192828PurposeExpresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoterDepositorInsertmTurquoise2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 for sgRNA and hepatocyte-specific promoter (HS…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-iPE-Plus
Plasmid#214062Purposeinducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locusDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA donor
Plasmid#22075PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertSA-Puro
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-GFP
Plasmid#80491Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expressionDepositorInsertCAG-GFP
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_AAVS1_T2
Plasmid#79888PurposeExpresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.DepositorTypeEmpty backboneUseCRISPRTagsUntagged eSpCas9(1.1)ExpressionMammalianMutationPromoterCBhAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-M2rtTA
Plasmid#60843PurposeAAVS1 donor vector for genomic targetingDepositorInsertsM2rtTA
Neo
UseTargeting donorTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ZFN-AAVS1
Plasmid#89707PurposeMammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locusDepositorInsertZFN-AAVS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-mCh
Plasmid#80492Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expressionDepositorInsertmCherry
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NC-CRISPRi (Gen3)
Plasmid#73499PurposeConstitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertdCas9-KRAB
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-GFPluc2
Plasmid#80493Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expressionDepositorInsertGFPluc2
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZFN-AAVS1-KKR
Plasmid#159298PurposeZFN (right) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertZFN targeting the AAVS1 locus
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-vpr
Plasmid#89985Purposeinducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locusDepositorInsertdCas9-VPR
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-AAVS1_B3903c_attP
Plasmid#182144PurposeTo insert attP attachment site at AAVS1 in human cells via twinPEDepositorInsertAAVS1_B3903c_attP pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-AAVS1_A3786c_attP
Plasmid#182143PurposeTo insert attP attachment site at AAVS1 in human cells via twinPEDepositorInsertA3786c-attP pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-sgAAVS1-HepmCherry
Plasmid#192827PurposeExpresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mCherry from hepatocyte-specific promoterDepositorInsertmCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 for sgRNA and hepatocyte-specific promoter (HS…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-35:AAVS1-mEGFP
Plasmid#91565PurposeHomology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-AAVS1-TALEN-L
Plasmid#159299PurposeTALEN (left) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertTALEN targeting the AAVS1
UseTALENTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAVS1-TALEN-R
Plasmid#159300PurposeTALEN (right) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertTALEN targeting the AAVS1
UseTALENTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only