We narrowed to 4,233 results for: Gca

1. pTX198

   Plasmid

   #89262

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsPDS-gRNA01
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

   Plasmid

   #188704

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   NFATC2 sgRNAs
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CROP_C9_Puro_GART

   Plasmid

   #183296

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets GART gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   GART
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBSU6-shTim23/CMV-eGFP

   Plasmid

   #89795

   Purpose

   Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

   Depositor

   Robert Friedlander

   Article

   Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

   Insert

   shRNA Tim23 (Timm23 Mouse)
   

   Use

   Tags

   eGFP under separate promoter

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOC5 Tubb3-GFP KI

   Plasmid

   #183440

   Purpose

   FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pColE1_sgRNA_M7NS_AmpR

   Plasmid

   #119157

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pABM-D4-miR30-CypA

   Plasmid

   #132934

   Purpose

   Lentivector for CypA knockdown and blasticidin resistance

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   CypA shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Flag-Tpr-Si

   Plasmid

   #60918

   Purpose

   Expresses Flag tagged nucleoporin Tpr resistant to siRNA GCACAACAGGATAAGGTTA

   Depositor

   Vinay Nandicoori

   Article

   Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

   Insert

   TPR (TPR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations in siRNA target region; E1…

   Promoter

   CMV

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTorPE-G-GECO1.1

   Plasmid

   #32467

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.1
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

   Expression

   Bacterial

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. double_sgRNA targeting e3 and e7

    Plasmid

    #190689

    Purpose

    sgRNAs targeting enhancer 3 and 7 of MYC separately

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNAs targeting enhancer 3 and 7 of MYC separately
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CROP_C9_Puro_FGFR1

    Plasmid

    #183289

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets FGFR1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    FGFR1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSR9.1347

    Plasmid

    #39540

    Depositor

    Katharina Strub

    Article

    Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

    Insert

    SRP9 (SRP9 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pORANGE Tubb3-4xGFP KI

    Plasmid

    #183445

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 4xGFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Geph UTR shRNA

    Plasmid

    #121070

    Purpose

    shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CROP_C9_Puro_ESR1

    Plasmid

    #183288

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets ESR1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    ESR1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSR-puro-Sec5

    Plasmid

    #31117

    Depositor

    Christopher Counter

    Article

    Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

    Insert

    Sec5 shRNA (EXOC2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

17. MS2_adRNA

    Plasmid

    #124624

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MS2_adRNA_RAB7A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSuper.Retro.Puro-PKN1 shRNA 4

    Plasmid

    #59978

    Purpose

    Knockdown of PKN1

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    PKN1 shRNA
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pBbdCas9S_Psyn-sgRNAmreB

    Plasmid

    #149653

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX458_RXRB_2

    Plasmid

    #104051

    Purpose

    Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RXRB gRNA (RXRB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PX458_ZGPAT_1

    Plasmid

    #86327

    Purpose

    Encodes gRNA for 3' target of human ZGPAT

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ZGPAT (ZGPAT Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSMP-SMYD2_2

    Plasmid

    #36350

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SMYD2 (SMYD2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pLL3.7 Mor2 shRNA

    Plasmid

    #59298

    Purpose

    3rd generation lentiviral vector expressing shRNA to mouse Opmr1

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

    Insert

    Mor2 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

24. GWF303

    Plasmid

    #133611

    Purpose

    CD95-1-2xPP7, NLS-PCP-DNCR2-P2a-BFP

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    CD95-1-2xPP7, PCP-DNCR2, BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GWF314

    Plasmid

    #133610

    Purpose

    CXCR4-com, NLS-com-GNCR1-P2a-BFP

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    CXCR4-6-com, com-GNCR1, BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTX201

    Plasmid

    #89265

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsYSA-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SEPT11 sh2 shRNA

    Plasmid

    #122023

    Purpose

    Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 shRNA (Sept11 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LEPG_shSik3.308

    Plasmid

    #125878

    Purpose

    Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    shRNA targeting Sik3
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gRNA[bTub].1026C

    Plasmid

    #112691

    Purpose

    express gRNA targeting bTub under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    dU6-3

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX-APRT-sg2

    Plasmid

    #107274

    Purpose

    APRT sgRNA-2 and Cas9 expression vector

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    APRT sgRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. lentiCRISPR - C9orf114 sgRNA 1

    Plasmid

    #70655

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against C9orf114
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pCRRNA-G0-R20

    Plasmid

    #101818

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCRRNA-G20-R0

    Plasmid

    #101807

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA-H1-sgRNA-hTZAP

    Plasmid

    #87186

    Purpose

    guideRNA targeting exon1 of human TZAP

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (ZBTB48 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EcPhnD90.L1∆∆AD.L297RL301R.pRSET

    Plasmid

    #33376

    Purpose

    a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

    Depositor

    Loren Looger

    Article

    Alicea et al J Mol Biol. 2011 Oct 12.

    Insert

    phnD based Phosphonate Biosensor (phnD E. coli)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted in place of…

    Promoter

    T7

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PX458_SOX5_1

    Plasmid

    #86357

    Purpose

    Encodes gRNA for 3' target of human SOX5

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against SOX5 (SOX5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX330-sgRNA_Dgcr8_2

    Plasmid

    #73526

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

38. shCDK6(2)

    Plasmid

    #73553

    Purpose

    shRNA(2) against CDK6

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pRCA360 - pBA904 Puro-T2A-GFP

    Plasmid

    #238164

    Purpose

    Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    Non-targeting sgRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-IARS2-tb

    Plasmid

    #232340

    Purpose

    Expresses human IARS2 with synonymous mutations (to prevent recognition by siRNA) in mammalian cells

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 synonymous mutations in the siRNA (ACUUGCAGUCAU…

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. esgRNA_NbmiR164e/h

    Plasmid

    #231157

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbmiR164e/h

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbmiR164e/h
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. esgRNA_SlMIR164b

    Plasmid

    #231153

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting SlMIR164b

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting SlMIR164b
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pYAMTr2GCsgSeLEU2

    Plasmid

    #224870

    Purpose

    Disruption of S. eubayanus type LEU2 genes

    Depositor

    Katsunori Suzuki

    Article

    Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

    Insert

    pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. gRNA_SRR124.5_tagBFP

    Plasmid

    #163753

    Purpose

    gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    gRNA_SRR124.5
    

    Use

    CRISPR

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid

    #221844

    Purpose

    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GACG and U6.3 chick

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LV-sgRNA-mCherry-Puro sgPIDD

    Plasmid

    #211528

    Purpose

    Deletes PIDD

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgPIDD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210744

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 4
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid

    #208979

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210742

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 2
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge